Optimization of CEST MRI for detection of bacteria

用于细菌检测的 CEST MRI 优化

基本信息

  • 批准号:
    9303352
  • 负责人:
  • 金额:
    $ 8.08万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-07-01 至 2018-04-30
  • 项目状态:
    已结题

项目摘要

It is highly desired to develop new non-invasive imaging approach for detecting bacterial infection with improved specificity and spatial-temporal resolution. Although to date there are a number of molecular imaging approaches have been demonstrated for bacterial infection in the preclinical animal models, there is clearly a gap between the demand of clinically-compatible imaging methods and conventional tagging strategies, which often require the use of metallic or radioactive contrast agents. To address this gap, we are developing a “non-labeled” (i.e., not radioactive, and not paramagnetic- or super-paramagnetic-) approach for detecting bacteria. In this approach bacterial cells are directly detected through their endogenous molecules using an innovative MRI technology called Chemical Exchange Saturation Transfer (CEST). The long-term goal of our research is to exploit this endogenous bacteria-specific CEST MRI signal to detect pathogenic bacteria and monitor the progress of bacterial infection. As initially demonstrated in solid tumors, we showed that CEST MRI could detect a therapeutic bacteria C. novyi-NT, a genetically modified bacteria strain currently in the Phase I clinical trial for treating solid tumors. Based on the preliminary data, we hypothesize that CEST MRI that detects the germination and proliferation of C. novyi-NT can be used as a non-invasive imaging biomarker for predicting the success of bacteriolytic therapy on glioma, a highly lethal brain tumor type. We plan to test our hypothesis through completing the following two specific aims: 1) To improve the specificity of bacterial CEST contrast using newly developed CEST technologies, and 2) To test and validate the optimized bacterial CEST MRI detection in the C. novyi-NT cancer therapy. We anticipate that accomplishing these aims will result in a highly translatable MRI technology specifically for bacterial detection, which could be used immediately as an imaging biomarker in the clinical trials of C.novyi-NT bacteriolytic therapy. More importantly, the knowledge gained from this project will enable a further expansion of using the proposed CEST MRI methods to the detection of many other types of bacteria, which will be enormously useful for the diagnosis and treatment monitoring of bacterial infection in deep organs, such as brain, which are currently difficult to detect.
非常需要开发新的非侵入性成像方法来检测细菌感染 提高特异性和时空分辨率。尽管迄今为止已经有许多分子 成像方法已被证明可以用于临床前动物模型中的细菌感染, 临床兼容的成像方法与传统标记的需求之间显然存在差距 策略,通常需要使用金属或放射性造影剂。为了解决这一差距,我们 正在开发一种“非标记”(即无放射性、非顺磁或超顺磁) 检测细菌的方法。在这种方法中,细菌细胞通过其 使用称为化学交换饱和的创新 MRI 技术来检测内源性分子 转机(欧洲中部夏令时间)。我们研究的长期目标是利用这种内源性细菌特异性 CEST MRI信号可检测病原菌并监测细菌感染的进展。和最初一样 在实体瘤中得到证实,我们表明 CEST MRI 可以检测治疗性细菌 C. novyi-NT, 一种转基因细菌菌株,目前正处于治疗实体瘤的一期临床试验中。基于 根据初步数据,我们假设 CEST MRI 可以检测萌发和增殖 C. novyi-NT 可用作预测溶菌成功的非侵入性成像生物标志物 神经胶质瘤的治疗,一种高度致命的脑肿瘤类型。我们计划通过完成以下任务来检验我们的假设 以下两个具体目标: 1)使用新方法提高细菌 CEST 对比的特异性 开发了 CEST 技术,以及 2) 测试和验证优化的细菌 CEST MRI 检测 C. novyi-NT 癌症疗法。我们预计,实现这些目标将带来高度 专门用于细菌检测的可翻译 MRI 技术,可立即用作 C.novyi-NT 溶菌疗法临床试验中的影像生物标志物。更重要的是, 从该项目中获得的知识将能够进一步扩展拟议的 CEST MRI 的使用 检测许多其他类型细菌的方法,这对于 脑等深部器官细菌感染的诊断和治疗监测 目前很难检测到。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Detecting acid phosphatase enzymatic activity with phenol as a chemical exchange saturation transfer magnetic resonance imaging contrast agent (PhenolCEST MRI).
  • DOI:
    10.1016/j.bios.2019.111442
  • 发表时间:
    2019-09
  • 期刊:
  • 影响因子:
    12.6
  • 作者:
    Jia Zhang;Yue Yuan;Zheng Han;Yuguo Li;P. V. van Zijl;Xing Yang;J. Bulte;Guanshu Liu
  • 通讯作者:
    Jia Zhang;Yue Yuan;Zheng Han;Yuguo Li;P. V. van Zijl;Xing Yang;J. Bulte;Guanshu Liu
Detection and Quantification of Hydrogen Peroxide in Aqueous Solutions Using Chemical Exchange Saturation Transfer.
使用化学交换饱和转移在水溶液中检测和定量过氧化氢。
  • DOI:
    10.1021/acs.analchem.7b01763
  • 发表时间:
    2017-07-18
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Ryoo D;Xu X;Li Y;Tang JA;Zhang J;van Zijl PCM;Liu G
  • 通讯作者:
    Liu G
MRI detection of bacterial brain abscesses and monitoring of antibiotic treatment using bacCEST.
  • DOI:
    10.1002/mrm.27180
  • 发表时间:
    2018-08
  • 期刊:
  • 影响因子:
    3.3
  • 作者:
    Liu J;Bai R;Li Y;Staedtke V;Zhang S;van Zijl PCM;Liu G
  • 通讯作者:
    Liu G
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Guanshu Liu其他文献

Guanshu Liu的其他文献

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{{ truncateString('Guanshu Liu', 18)}}的其他基金

MPI/MRI bimodal imaging for non-invasive tracking of extracellular vesicles targeted to infarcted myocardium
MPI/MRI 双模成像,用于无创追踪梗塞心肌细胞外囊泡
  • 批准号:
    10557225
  • 财政年份:
    2022
  • 资助金额:
    $ 8.08万
  • 项目类别:
MPI/MRI bimodal imaging for non-invasive tracking of extracellular vesicles targeted to infarcted myocardium
MPI/MRI 双模成像,用于无创追踪梗塞心肌细胞外囊泡
  • 批准号:
    10366590
  • 财政年份:
    2022
  • 资助金额:
    $ 8.08万
  • 项目类别:
CEST MRI assessment of tumor vascular permeability using non-labeled dextrans
使用非标记葡聚糖评估肿瘤血管通透性的 CEST MRI
  • 批准号:
    9297917
  • 财政年份:
    2017
  • 资助金额:
    $ 8.08万
  • 项目类别:
Noninvasive prediction of tumor response to gemcitabine using MRI
使用 MRI 无创预测肿瘤对吉西他滨的反应
  • 批准号:
    9328948
  • 财政年份:
    2017
  • 资助金额:
    $ 8.08万
  • 项目类别:
Monitoring Prodrug Delivery in Suicide Gene Therapy Using CEST MRI
使用 CEST MRI 监测自杀基因治疗中的前药递送
  • 批准号:
    8510646
  • 财政年份:
    2012
  • 资助金额:
    $ 8.08万
  • 项目类别:
Monitoring Prodrug Delivery in Suicide Gene Therapy Using CEST MRI
使用 CEST MRI 监测自杀基因治疗中的前药递送
  • 批准号:
    8356569
  • 财政年份:
    2012
  • 资助金额:
    $ 8.08万
  • 项目类别:

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    2140205
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受阻酰胺的天体选择性合成-合成肽催化剂的探索-
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    WBP Fellowship
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镍催化仲酰胺的α-芳基化
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Catalytic Synthesis of Pharmaceutical Amides in Water
水中催化合成药用酰胺
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