Regulation of Bacterial Aggregation During Host Colonization

宿主定植过程中细菌聚集的调节

基本信息

  • 批准号:
    9541588
  • 负责人:
  • 金额:
    $ 16.67万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-03-10 至 2019-02-28
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): Microorganisms are able to switch from a planktonic, freeliving lifestyle in the environment to an aggregated, biofilm-associated state upon animal colonization. Across many organisms, this transition is associated with increased levels of cyclic dimeric GMP (c-di-GMP), an intracellular second messenger that coordinates the downregulation of motility genes and the upregulation of biofilm genes during the transition from the motile to the sessile lifestyle. Sessile, biofilm embedded bacterial colonies underlie chronic bacterial pathogenesis and bacteria within biofilms exhibit increased resistance to antibacterial treatments. Mechanisms regulating c-di- GMP turnover have been described, and the compound influences output phenotypes in various ways, including direct binding to proteins and to regulatory RNA. Previous work has relied on sophisticated systems in bacterial culture, eukaryotic cell culture, and animal models. However, little is known about the processes by which interaction with an intact animal stimulates a change in bacterial signal transduction. To understand the processes by which host-microbe interaction directs bacteria to adopt a sessile lifestyle, it is useful to study a model system in which individual stages can be dissected through genetics and imaging approaches. The light organ of the bobtail squid, Euprymna scolopes, is colonized exclusively by one bacterial species, Vibrio fischeri. Colonization is robust: it occurs within four hours via the native route and in the presence of host immune factors. Biofilm formation is required for initiating a successful colonization, and bacteria do not progress past this initial phase if they fail to aggregate in host mucus. We identified a bacterial mutant that dramatically increases aggregation behavior. The genetic lesion mapped to a hybrid histidine kinase, VF_A0360, and in addition to conferring increased aggregation the mutant also exhibited reduced flagellar motility and higher levels of c-di-GMP. Together, these data support the hypothesis that signal transduction through VF_A0360 coordinates the transition from the environmental state to the host-associated colonized state, and here we examine the following specific aims: (1) Identify genes regulated by the VF_A0360 histidine kinase and examine their dynamics during entry to and exit from squid colonization, and (2) Characterize the phosphorelay through which VF_A0360 regulates biofilm formation. By interrogating this signaling pathway we will gain insight into how bacterial signal transduction adjusts to the host environment during beneficial and pathogenic colonization.


项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Optimization of a minimal sample preparation protocol for imaging mass spectrometry of unsectioned juvenile invertebrates.
用于未切片幼年无脊椎动物成像质谱分析的最小样品制备方案的优化。
  • DOI:
    10.1002/jms.4458
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Zink,KatherineE;Tarnowski,DeniseA;Mandel,MarkJ;Sanchez,LauraM
  • 通讯作者:
    Sanchez,LauraM
D-fining DarR: a LysR-type transcriptional regulator that responds to D-aspartate.
D-fining DarR:一种对 D-天冬氨酸有反应的 LysR 型转录调节因子。
  • DOI:
    10.1128/jb.00121-18
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    3.2
  • 作者:
    Mandel,MarkJ
  • 通讯作者:
    Mandel,MarkJ
Impact and Influence of the Natural Vibrio-Squid Symbiosis in Understanding Bacterial-Animal Interactions.
  • DOI:
    10.3389/fmicb.2016.01982
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    5.2
  • 作者:
    Mandel MJ;Dunn AK
  • 通讯作者:
    Dunn AK
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Mark J Mandel其他文献

Mark J Mandel的其他文献

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{{ truncateString('Mark J Mandel', 18)}}的其他基金

Genetic analysis of beneficial bacterial colonization
有益细菌定植的遗传分析
  • 批准号:
    10555034
  • 财政年份:
    2023
  • 资助金额:
    $ 16.67万
  • 项目类别:
28th Annual Midwest Microbial Pathogenesis Conference (MMPC)
第 28 届年度中西部微生物发病机制会议 (MMPC)
  • 批准号:
    10540505
  • 财政年份:
    2022
  • 资助金额:
    $ 16.67万
  • 项目类别:
Genetic Analysis of Beneficial Bacterial Colonization
有益细菌定植的遗传分析
  • 批准号:
    10384521
  • 财政年份:
    2016
  • 资助金额:
    $ 16.67万
  • 项目类别:
Genetic Analysis of Beneficial Bacterial Colonization
有益细菌定植的遗传分析
  • 批准号:
    9562759
  • 财政年份:
    2016
  • 资助金额:
    $ 16.67万
  • 项目类别:
Genetic Analysis of Beneficial Bacterial Colonization
有益细菌定植的遗传分析
  • 批准号:
    10217519
  • 财政年份:
    2016
  • 资助金额:
    $ 16.67万
  • 项目类别:
Symbiotic colonization of host mucus by Vibrio fischeri
费氏弧菌在宿主粘液中的共生定殖
  • 批准号:
    7455967
  • 财政年份:
    2006
  • 资助金额:
    $ 16.67万
  • 项目类别:
Symbiotic colonization of host mucus by Vibrio fischeri
费氏弧菌在宿主粘液中的共生定殖
  • 批准号:
    7258844
  • 财政年份:
    2006
  • 资助金额:
    $ 16.67万
  • 项目类别:
Symbiotic colonization of host mucus by Vibrio fischeri
费氏弧菌在宿主粘液中的共生定殖
  • 批准号:
    7154414
  • 财政年份:
    2006
  • 资助金额:
    $ 16.67万
  • 项目类别:

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