Sensory Encoding in Hair Cells of the Zebrafish Lateral Line

斑马鱼侧线毛细胞的感觉编码

• 批准号: 9171195
• 负责人: Josef George Trapani
• 金额: $ 43.94万
• 依托单位: AMHERST COLLEGE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9171195
• 关键词: Action Potentials; Afferent Neurons; Auditory; Auditory Perception; Auditory system; Brain; Bypass; Cochlear Implants; Communication impairment; Data; Dependence; Development; Devices; Dose; Electrophysiology (science); Frequencies; Future; Goals; Hair Cells; Hearing problem; Human; Ion Channel; Knowledge; Laboratories; Larva; Lead; Light; Location; Mammals; Mechanics; Medical Device; Modality; Music; Neurons; Optics; Outcome; Output; Pattern; Perception; Phase; Process; Proteins; Reflex action; Research; Research Personnel; Role; Sensory; Sensory Hair; Sensory Receptors; Speech; Stimulus; Study models; Synaptic Vesicles; System; Testing; Time; Training; Transgenic Organisms; Validation; Vertebrates; Voice; Work; Zebrafish; college; deafness; equilibration disorder; hearing impairment; in vivo; innovation; insight; lateral line; light gated; neurophysiology; optogenetics; programs; receptor; research study; response; ribbon synapse; sound; sound frequency; stimulus interval; transmission process; undergraduate student

The process by which the auditory system encodes low-frequency sounds such as those produced by the human voice and a grand piano is not entirely understood. Our long-term goal is to determine how hair cell sensory receptors encode stimulus features into representative sequences of electrical activity in sensory afferent neurons. Understanding this process relies on understanding how intrinsic hair-cell mechanisms contribute to encoded activity. The overall objective of this proposal is to examine sensory encoding in the lateral line system of larval zebrafish. Specifically, the central hypothesis of this proposal is that lateral-line hair cells encode stimulus intensity and duration within defined temporal patterns of afferent activity. The hypothesis was formulated through preliminary experiments on both wild type and transgenic larvae with hair-cell expression of the optogenetic protein, channelrhodhopsin-2 (ChR2), which allows for optical stimulation of hair cells instead of via activation of MET channels with mechanical stimuli. The central hypothesis will be tested with the following two specific aims: 1) Determine the relationship between stimulus features and activity parameters, which include the onset of activity, total amount of activity and the temporal patterns of the activity in wild type larvae; and 2) Compare neuron activity parameters between mechanical and optical stimulation of hair cells in transgenic larvae. Aim 1 will examine the dependence of activity parameters during manipulation of stimulus intensity, duration, and repetition. Experiments in Aim 2 will compare mechanically and optically evoked activity in two different transgenic zebrafish lines that combine optogenetics with wild type data to provide insight into the contribution of intrinsic hair-cell mechanisms on the parameters of afferent neuron activity. The rationale for this proposal is that examination of the relationship between input stimulus features and output activity parameters will further our understanding of auditory perception. The approach is innovative as it utilizes an in vivo approach with electrophysiology on single afferent neurons together with optogenetics to investigate low-frequency encoding in an intact larval zebrafish. Given the importance of low frequency auditory information, and that hair cells are remarkably conserved across vertebrates, our findings will be significant for understanding the function of the mammalian auditory system and will have a positive impact on the treatment of deafness and communication disorders, including the development of cochlear implants that can reliably encode low-frequency information.

听觉系统对低频声音进行编码的过程

项目成果

Mathematical Modeling and Analyses of Interspike-Intervals of Spontaneous Activity in Afferent Neurons of the Zebrafish Lateral Line.

• DOI: 10.1038/s41598-018-33064-z
• 发表时间: 2018-10-05
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Song S;Lee JA;Kiselev I;Iyengar V;Trapani JG;Tania N
• 通讯作者: Tania N

Teaching Dose-response Relationships Through Aminoglycoside Block of Mechanotransduction Channels in Lateral Line Hair Cells of Larval Zebrafish.

通过氨基糖苷阻断斑马鱼幼虫侧线毛细胞的机械传导通道来教学剂量-反应关系。

• 发表时间: 2018
• 期刊: Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience
• 作者: Peterson,HannahPayette;Troconis,EileenL;Ordoobadi,AlexanderJ;Thibodeau-Beganny,Stacey;Trapani,JosefG
• 通讯作者: Trapani,JosefG

A sensation for inflation: initial swim bladder inflation in larval zebrafish is mediated by the mechanosensory lateral line.

膨胀的感觉：斑马鱼幼体的初始鱼鳔膨胀是由机械感觉侧线介导的。

• DOI: 10.1101/2023.01.12.523756
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Venuto,Alexandra;Thibodeau-Beganny,Stacey;Trapani,JosefG;Erickson,Timothy
• 通讯作者: Erickson,Timothy

Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish.

• DOI: 10.1007/978-1-0716-0830-2_13
• 发表时间: 2021
• 期刊: Methods in molecular biology
• 作者: Y. Ozdemir;C. A. Hansen;Mohamed Ramy;Eileen L. Troconis;Lauren D McNeil;Josef G. Trapani