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The role of Dzip1L in ciliogenesis and Hedgehog signaling

Dzip1L 在纤毛发生和 Hedgehog 信号传导中的作用

基本信息

批准号：
9244039
负责人：
BAOLIN WANG
金额：
$ 35.6万
依托单位：
WEILL MEDICAL COLL OF CORNELL UNIV
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-04-01 至 2019-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9244039
关键词：
Activator Appliances Affect Alleles Apical Biological Birth Brain C-terminal Cell membrane Cell surface Cells Centrioles Centrosome Cilia Defect Disease Docking Embryo Environment Epithelial Cells Erinaceidae Exhibits Fibroblasts Functional disorder Gene Mutation Genes Genetic Genetic study Golgi Apparatus Growth Human Iguanas Immunofluorescence Immunologic Knockout Mice Length Light Limb Bud Limb structure Mesenchymal Microtubules Midbrain structure Molecular Morphology Mothers Mus Mutant Strains Mice Mutate Mutation Neural tube Neuroepithelial Neuroepithelial Cells Organelles Pathway interactions Pattern Phenotype Polydactyly Process Prosencephalon Proteins Regulation Role Sensory Signal Transduction Signaling Molecule Smooth Muscle Myocytes Stretching Structure Time Tissues Transmission Electron Microscopy Vesicle Western Blotting Zebrafish base cilium biogenesis drug development extracellular human disease insight kinetosome mutant progenitor public health relevance smoothened signaling pathway therapeutic target transcription factor

项目摘要

DESCRIPTION (provided by applicant): The primary cilium is a solitary microtubule-based organelle that protrudes from the cell surface and is found on most vertebrate cells. Primary cilia originate in the mother centriole of the centrosome and are elongated and maintained by intraflagellar transport (IFT). Previous transmission electron microscopy (TEM) studies defined two different ciliogenesis pathways for primary cilia. In epithelial cells, the mother centriole appears to dock directly at the apical plasma membrane; from there, the axoneme grows out toward the extracellular environment. This is called the extracellular pathway. In contrast, in fibroblasts and smooth muscle cells, the cilium appears to first grow within the cell body, upon docking of the mother centriole to a Golgi-derived primary ciliary vesicle. The growth of the axoneme takes place within this vesicle. Fusion of this vesicle with the plasma membrane finally allows the cilium to access the extracellular environment. This is called the intracellular pathway. Thus far, nothing is known about how these two pathways are differentially regulated at the molecular levels. Ciliary dysfunction disrupts Hedgehog (Hh) signaling by affecting the activity of the Gli2 and Gli3 transcription factors, two primary regulators of Hh signaling. Most known ciliary gene mutations identified thus far result in failed Gli2 activation and decreased Gli3 repressor levels, but the underlying molecular mechanisms remain poorly understood. We recently mutated the mouse Dzip1L gene, which encodes a centriolar protein. Dzip1L mutant mice exhibit polydactyly and an enlarged forebrain and midbrain. Loss of Dzip1L results in reduced Gli3 repressor activity but does not affect Gli2 activation. Surprisingly, although cilia ae normal in neuroepithelial cells of the Dzip1L mutant brain, there is a pronounced paucity of cilia in mutant limb bud mesenchymal cells and embryonic fibroblasts. Thus, Dzip1L is the first known cilia-related protein that specifically regulates ciliogenesis in fibroblasts, but not epitheial cells, and affects only Gli3 processing but not Gli2 activation. Our objective is to understand how Dzip1L mutation affects Gli3 processing but not Gli2 activation and how Dzip1L differentially regulates the two ciliogenesis pathways using genetic, molecular, and cell biological approaches.

描述(申请人提供)：初级纤毛是一种从细胞表面伸出的以微管为基础的细胞器，存在于大多数脊椎动物细胞中。初生纤毛起源于中心体的母中心粒，由鞭毛内运输(IFT)延长和维持。以前的透射电子显微镜研究为初生纤毛定义了两种不同的纤毛发生途径。在上皮细胞中，母中心粒似乎直接停靠在顶端质膜上；轴丝从那里向细胞外环境生长。这就是所谓的细胞外途径。相反，在成纤维细胞和平滑肌细胞中，纤毛似乎首先在细胞体内生长，当母中心粒与高尔基体衍生的初级纤毛小泡对接时。轴丝的生长发生在这个小泡内。这种囊泡与质膜的融合最终允许纤毛进入细胞外环境。这被称为细胞内途径。到目前为止，对于这两条通路是如何在分子水平上进行差异调控的，人们还一无所知。睫状体功能障碍通过影响Gli2和Gli3转录因子的活性来干扰Hedgehog(HH)信号，Gli2和Gli3是HH信号的两个主要调节因子。到目前为止，大多数已知的纤毛基因突变导致Gli2激活失败和Gli3抑制物水平降低，但其潜在的分子机制仍然知之甚少。我们最近突变了小鼠的Dzip1L基因，它编码一种中心粒蛋白。Dzip1L突变小鼠表现为多指，前脑和中脑增大。Dzip1L的缺失会导致Gli3抑制物活性降低，但不会影响Gli2的激活。令人惊讶的是，虽然Dzip1L突变脑的神经上皮细胞中纤毛正常，但突变的肢芽间充质细胞和胚胎成纤维细胞中纤毛明显稀少。因此，Dzip1L是第一个已知的纤毛相关蛋白，它特异性地调节成纤维细胞的纤毛发生，而不是附壁细胞，并且只影响Gli3的加工，而不影响Gli2的激活。我们的目标是了解Dzip1L突变是如何影响Gli3加工而不是Gli2激活的，以及Dzip1L如何利用遗传、分子和细胞生物学方法对两条纤毛发生途径进行差异调节。