CACHET: Biomarkers Core

CACHET：生物标志物核心

• 批准号: 9271016
• 负责人: Jack Anthony Gilbert
• 金额: $ 4.28万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9271016
• 关键词: Anaerobic Bacteria; Androgens; Animals; Biological Assay; Biological Markers; Carbazoles; Chicago; Collaborations; Core Facility; Coupled; DNA Adducts; DNA Markers; Detection; Development; Dinoprostone; Endocrine Disruptors; Environment; Environmental Impact; Estrogens; Exposure to; Flame Retardants; Focus Groups; Food; Gas Chromatography; Health; Heavy Metals; High Pressure Liquid Chromatography; Human; Human Microbiome; Illinois; Individual; Inductively Coupled Plasma Mass Spectrometry; Isoprostanes; Laboratories; Lipid Peroxidation; Lipid Peroxides; Liquid Chromatography; Liquid substance; Mass Fragmentography; Measurement; Measures; Metagenomics; Metals; Methods; Needs Assessment; Nose; Oral cavity; Organic Chemicals; Oxidative Stress; Performance; Pesticides; Physiological; Polychlorinated Biphenyls; Procedures; Prostaglandin D2; Prostaglandins; Proteomics; Research; Research Personnel; Resources; Role; Sampling; Serum; Services; Skin; Soil; Source; Specimen; Stimulus; Technology; Tissues; Toxic Environmental Substances; Toxin; Universities; Urine; Validation; Water; analytical method; base; bisphenol A; design; dibenzofuran; discount; environmental chemical; experience; inflammatory marker; liquid chromatography mass spectrometry; member; metabolomics; microbial; microbiome; next generation sequencing; organochlorine pesticide; oxidation; oxidized lipid; pollutant; pressure; response; tandem mass spectrometry; toxicant; transcriptomics; urinary

1. BIOMARKERS CORE: ABSTRACT The Biomarkers Core provides essential analytical measurements in support of all focus groups of the ChicAgo Center for Health and EnvironmenT (CACHET). The overarching objectives of this Core are to provide CACHET members access to the following: a) routine analytical support enabling determination of exposure to environmental toxins as well as biomarkers indicating the physiological consequences of this exposure; b) new analytical methods and access to resources not previously available to CACHET members; and c) expertise for the design and interpretation of analytical measurements. Core access will be available equally and at discounted rates to all CACHET members, regardless of their institutional affiliation. Types of analyses and services will include 1) quantitative analysis of toxicants (such as pesticides and endocrine disruptors like bisphenol A) in biomedical specimens, and related biomarkers of exposure (including markers of inflammation such as the prostaglandins PGE2 and PGD2 and markers of oxidative stress such as 8-oxo-dG and 8-isoprostane PGF2α) using ultrahigh-pressure liquid chromatography combined with high performance tandem mass spectrometry (UHPLC-MS/MS) in the laboratory of Core Leader Dr. Richard van Breemen at UIC; 2) trace quantitative analysis of semi-volatile organic toxins such as PAHs, organochlorine pesticides, polyhalogenated carbazoles, and halogenated flame retardants in human and environmental samples using gas chromatography-tandem mass spectrometry (GC-MS/MS) in the laboratory of Dr. An Li at UIC; 3) quantitative analysis of metals (including Al, Mn, Fe, Ni, Cu, Zn, As, Se, Mo, Cd, Sn, Sb, Hg, and Pb) in environmental and biomedical specimens by using inductively coupled plasma mass spectrometry (ICP-MS) in the laboratory of Dr. C. Jin Qin at UofC; and 4) characterizing effects of environmental toxicants on the human microbiome in the laboratory of microbial ecologist Dr. Jack Gilbert at UofC. Some of these core resources, such as GC-MS/MS analysis of semi-volatile environmental toxins and effects of environmental toxins on the human microbiome have not been accessible previously to researchers at either UIC or UofC. Due to institutional contributions and CACHET support, these resources will be readily available to CACHET members at substantial discounts. Each laboratory providing services within this core is directed by an expert, and all of these resources will be coordinated by Dr. van Breemen, who has over 30 years of experience in the management of core facilities including MS-based resources.

1.生物标志物核心：摘要 生物标志物核心提供了必要的分析测量，以支持芝加哥会议的所有焦点小组。 健康与环境中心（CACHET）。该核心的总体目标是提供CACHET a）例行分析支助，以便确定 环境毒素以及指示这种暴露的生理后果的生物标志物; B）新的 分析方法和获得CACHET成员以前无法获得的资源的机会;以及 分析测量的设计和解释。核心访问将平等提供， 所有CACHET成员，无论其机构隶属关系，折扣率。分析类型和 服务将包括1）有毒物质的定量分析（例如农药和内分泌干扰物， 生物医学样本中的双酚A）和相关的暴露生物标志物（包括炎症标志物 例如前列腺素PGE 2和PGD 2以及氧化应激的标记物例如8-氧代-dG和8-异前列烷 超高压液相色谱-高效串联质谱法测定前列腺素F2 α UIC核心负责人Richard货车Breemen博士实验室中的超高效液相色谱-串联质谱法（UHPLC-MS/MS）; 2）痕量 半挥发性有机毒素如多环芳烃、有机氯农药、多卤代 咔唑和卤代阻燃剂在人体和环境样品中使用气体 色谱-串联质谱（GC-MS/MS）在UIC的An Li博士实验室进行; 3）定量 环境中的金属（包括Al、Mn、Fe、Ni、Cu、Zn、As、Se、Mo、Cd、Sn、Sb、Hg和Pb）分析， 生物医学标本通过使用电感耦合等离子体质谱法（ICP-MS）在博士的实验室。 C.金琴在UofC;和4）表征环境毒物对人类微生物组的影响， 微生物生态学家杰克吉尔伯特博士实验室。其中一些核心资源，如GC-MS/MS 分析半挥发性环境毒素和环境毒素对人体微生物组的影响， 以前UIC或UofC的研究人员都无法获得。由于机构捐款和 CACHET支持，这些资源将随时提供给CACHET成员在大幅折扣。每个 在此核心内提供服务的实验室由专家指导，所有这些资源将 由拥有30多年核心设施管理经验的货车Breemen博士协调 包括基于MS的资源。