CACHET: Biomarkers Core

CACHET：生物标志物核心

• 批准号: 9572579
• 负责人: Jack Anthony Gilbert
• 金额: $ 5.26万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9572579
• 关键词: Anaerobic Bacteria; Androgens; Animals; Biological Assay; Biological Markers; Carbazoles; Chicago; Collaborations; Core Facility; Coupled; DNA Adducts; DNA Markers; Detection; Development; Dinoprostone; Endocrine Disruptors; Environment; Environmental Impact; Estrogens; Exposure to; Flame Retardants; Focus Groups; Food; Gas Chromatography; Health; Heavy Metals; High Pressure Liquid Chromatography; Human; Human Microbiome; Illinois; Individual; Inductively Coupled Plasma Mass Spectrometry; Isoprostanes; Laboratories; Lipid Peroxidation; Liquid Chromatography; Liquid substance; Mass Fragmentography; Measurement; Measures; Metagenomics; Metals; Methods; Needs Assessment; Nose; Oral cavity; Organic Chemicals; Oxidative Stress; Performance; Pesticides; Physiological; Polychlorinated Biphenyls; Procedures; Prostaglandin D2; Prostaglandins; Proteomics; Research; Research Personnel; Resources; Role; Sampling; Serum; Services; Skin; Soil; Source; Specimen; Stimulus; Technology; Tissues; Toxic Environmental Substances; Toxin; Universities; Urine; Validation; Water; analytical method; base; bisphenol A; design; dibenzofuran; discount; environmental chemical; experience; inflammatory marker; liquid chromatography mass spectrometry; member; metabolomics; metatranscriptomics; microbial; microbiome; next generation sequencing; organochlorine pesticide; oxidation; oxidized lipid; pollutant; pressure; response; tandem mass spectrometry; toxicant; transcriptomics; urinary

1. BIOMARKERS CORE: ABSTRACT The Biomarkers Core provides essential analytical measurements in support of all focus groups of the ChicAgo Center for Health and EnvironmenT (CACHET). The overarching objectives of this Core are to provide CACHET members access to the following: a) routine analytical support enabling determination of exposure to environmental toxins as well as biomarkers indicating the physiological consequences of this exposure; b) new analytical methods and access to resources not previously available to CACHET members; and c) expertise for the design and interpretation of analytical measurements. Core access will be available equally and at discounted rates to all CACHET members, regardless of their institutional affiliation. Types of analyses and services will include 1) quantitative analysis of toxicants (such as pesticides and endocrine disruptors like bisphenol A) in biomedical specimens, and related biomarkers of exposure (including markers of inflammation such as the prostaglandins PGE2 and PGD2 and markers of oxidative stress such as 8-oxo-dG and 8-isoprostane PGF2α) using ultrahigh-pressure liquid chromatography combined with high performance tandem mass spectrometry (UHPLC-MS/MS) in the laboratory of Core Leader Dr. Richard van Breemen at UIC; 2) trace quantitative analysis of semi-volatile organic toxins such as PAHs, organochlorine pesticides, polyhalogenated carbazoles, and halogenated flame retardants in human and environmental samples using gas chromatography-tandem mass spectrometry (GC-MS/MS) in the laboratory of Dr. An Li at UIC; 3) quantitative analysis of metals (including Al, Mn, Fe, Ni, Cu, Zn, As, Se, Mo, Cd, Sn, Sb, Hg, and Pb) in environmental and biomedical specimens by using inductively coupled plasma mass spectrometry (ICP-MS) in the laboratory of Dr. C. Jin Qin at UofC; and 4) characterizing effects of environmental toxicants on the human microbiome in the laboratory of microbial ecologist Dr. Jack Gilbert at UofC. Some of these core resources, such as GC-MS/MS analysis of semi-volatile environmental toxins and effects of environmental toxins on the human microbiome have not been accessible previously to researchers at either UIC or UofC. Due to institutional contributions and CACHET support, these resources will be readily available to CACHET members at substantial discounts. Each laboratory providing services within this core is directed by an expert, and all of these resources will be coordinated by Dr. van Breemen, who has over 30 years of experience in the management of core facilities including MS-based resources.

1.生物标志物核心：摘要 Biomarkers核心提供基本的分析测量，以支持芝加哥的所有重点小组 健康与环境中心(公章)。这个核心的首要目标是提供声望 成员可获得以下信息：a)例行分析支持，以便能够确定暴露于 表明这种接触的生理后果的环境毒素和生物标志物；b)新的 分析方法和获得以前没有的资源；以及c)专门知识 分析测量的设计和解释。核心接入将以同样的方式提供 向所有尊贵会员提供折扣率，无论其机构归属如何。分析的类型和 服务将包括1)对毒物(如杀虫剂和内分泌干扰物，如 生物医学标本中的双酚A)和相关的暴露生物标志物(包括炎症标志物 如前列腺素PGE2和PGD2，以及氧化应激的标志物，如8-氧-DG和8-异前列腺素 PGF_2α)的超高压液相色谱-高效串联质谱法 UIC核心领导理查德·范·布里曼博士的实验室中的光谱分析(UHPLC-MS/MS)；2)跟踪 多卤化多环芳烃、有机氯农药等半挥发性有机毒素的定量分析 人体和环境样品中的咔唑和卤化阻燃剂 气相色谱-串联质谱仪(GC-MS/MS)在中国医科大学安莉博士实验室的应用；3)定量 环境和环境中金属(包括铝、锰、铁、镍、铜、锌、砷、硒、钼、镉、锡、锑、汞和铅)的分析 生物医学标本的电感耦合等离子体质谱(电感耦合等离子体质谱)在博士的实验室。 以及4)表征环境毒物对人体微生物群的影响。 加州大学微生物生态学家杰克·吉尔伯特博士的实验室。其中一些核心资源，如GC-MS/MS 半挥发性环境毒素的分析及其对人体微生物群的影响 UIC或UofC的研究人员以前都无法访问。由于机构捐款和 纪念碑支持，这些资源将随时提供给纪念碑会员大幅折扣。每个人 在这个核心内提供服务的实验室由一名专家指导，所有这些资源都将 由范·布里曼博士协调，他在核心设施的管理方面有30多年的经验 包括基于MS的资源。