CACHET: Biomarkers Core

CACHET：生物标志物核心

• 批准号: 9572579
• 负责人: Jack Anthony Gilbert
• 金额: $ 5.26万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9572579
• 关键词: Anaerobic Bacteria; Androgens; Animals; Biological Assay; Biological Markers; Carbazoles; Chicago; Collaborations; Core Facility; Coupled; DNA Adducts; DNA Markers; Detection; Development; Dinoprostone; Endocrine Disruptors; Environment; Environmental Impact; Estrogens; Exposure to; Flame Retardants; Focus Groups; Food; Gas Chromatography; Health; Heavy Metals; High Pressure Liquid Chromatography; Human; Human Microbiome; Illinois; Individual; Inductively Coupled Plasma Mass Spectrometry; Isoprostanes; Laboratories; Lipid Peroxidation; Liquid Chromatography; Liquid substance; Mass Fragmentography; Measurement; Measures; Metagenomics; Metals; Methods; Needs Assessment; Nose; Oral cavity; Organic Chemicals; Oxidative Stress; Performance; Pesticides; Physiological; Polychlorinated Biphenyls; Procedures; Prostaglandin D2; Prostaglandins; Proteomics; Research; Research Personnel; Resources; Role; Sampling; Serum; Services; Skin; Soil; Source; Specimen; Stimulus; Technology; Tissues; Toxic Environmental Substances; Toxin; Universities; Urine; Validation; Water; analytical method; base; bisphenol A; design; dibenzofuran; discount; environmental chemical; experience; inflammatory marker; liquid chromatography mass spectrometry; member; metabolomics; metatranscriptomics; microbial; microbiome; next generation sequencing; organochlorine pesticide; oxidation; oxidized lipid; pollutant; pressure; response; tandem mass spectrometry; toxicant; transcriptomics; urinary

1. BIOMARKERS CORE: ABSTRACT The Biomarkers Core provides essential analytical measurements in support of all focus groups of the ChicAgo Center for Health and EnvironmenT (CACHET). The overarching objectives of this Core are to provide CACHET members access to the following: a) routine analytical support enabling determination of exposure to environmental toxins as well as biomarkers indicating the physiological consequences of this exposure; b) new analytical methods and access to resources not previously available to CACHET members; and c) expertise for the design and interpretation of analytical measurements. Core access will be available equally and at discounted rates to all CACHET members, regardless of their institutional affiliation. Types of analyses and services will include 1) quantitative analysis of toxicants (such as pesticides and endocrine disruptors like bisphenol A) in biomedical specimens, and related biomarkers of exposure (including markers of inflammation such as the prostaglandins PGE2 and PGD2 and markers of oxidative stress such as 8-oxo-dG and 8-isoprostane PGF2α) using ultrahigh-pressure liquid chromatography combined with high performance tandem mass spectrometry (UHPLC-MS/MS) in the laboratory of Core Leader Dr. Richard van Breemen at UIC; 2) trace quantitative analysis of semi-volatile organic toxins such as PAHs, organochlorine pesticides, polyhalogenated carbazoles, and halogenated flame retardants in human and environmental samples using gas chromatography-tandem mass spectrometry (GC-MS/MS) in the laboratory of Dr. An Li at UIC; 3) quantitative analysis of metals (including Al, Mn, Fe, Ni, Cu, Zn, As, Se, Mo, Cd, Sn, Sb, Hg, and Pb) in environmental and biomedical specimens by using inductively coupled plasma mass spectrometry (ICP-MS) in the laboratory of Dr. C. Jin Qin at UofC; and 4) characterizing effects of environmental toxicants on the human microbiome in the laboratory of microbial ecologist Dr. Jack Gilbert at UofC. Some of these core resources, such as GC-MS/MS analysis of semi-volatile environmental toxins and effects of environmental toxins on the human microbiome have not been accessible previously to researchers at either UIC or UofC. Due to institutional contributions and CACHET support, these resources will be readily available to CACHET members at substantial discounts. Each laboratory providing services within this core is directed by an expert, and all of these resources will be coordinated by Dr. van Breemen, who has over 30 years of experience in the management of core facilities including MS-based resources.

1。生物标志物核心：摘要 生物标志物核心提供基本的分析测量，以支持芝加哥的所有焦点小组 健康与环境中心（CACHET）。该核心的总体对象是提供Cachet 成员访问以下内容：a）常规分析支持以确定暴露于 环境毒素以及生物标志物表明这种暴露的物理后果； b）新 分析方法和对Cachet成员以前无法使用的资源的访问； c）专业知识 分析测量的设计和解释。核心访问将平等可用，在 不论其机构会员如何，所有Cachet成员的折扣利率。分析类型和 服务将包括1）对有毒物质的定量分析（例如农药和内分泌破坏者，例如 双苯酚A）在生物医学标本中和相关的暴露生物标志物（包括炎症标志物 例如前列腺素PGE2和PGD2以及氧化应激的标记，例如8-氧和8-异前列腺素 PGF2α）使用超压力液色谱法与高性能串联质量结合 核心领导者Richard Van Breemen博士在UIC的实验室中的光谱法（UHPLC-MS/MS）； 2）跟踪 半挥发性有机毒素（例如PAH，有机氯农药，多增生的）的定量分析 使用气体中的甲状化和环境样品中的卤素阻燃剂 在UIC的A. An Li博士实验室中的色谱串数据质谱（GC-MS/MS）； 3）定量 在环境和 生物医学标本通过在博士实验室中使用电感耦合等离子体质谱（ICP-MS） UOFC的C. Jin Qin; 4）表征环境毒物对人类微生物组的影响 UOFC的微生物生态学家Jack Gilbert博士实验室。其中一些核心资源，例如GC-MS/MS 半挥发性环境毒素的分析以及环境毒素对人类微生物组的影响 以前在UIC或UOFC的研究人员都无法访问。由于机构的贡献和 CACHET支持，这些资源将很容易以大量的折扣提供给Cachet成员。每个 在此核心内提供服务的实验室由专家指导，所有这些资源都将是 由Van Breemen博士协调，他在核心设施管理方面拥有30多年的经验 包括基于MS的资源。