Plasticity in the CNS of blind fish after eye regeneration

眼睛再生后盲鱼中枢神经系统的可塑性

• 批准号: 9305344
• 负责人: DAPHNE SOARES
• 金额: $ 43.07万
• 依托单位: NEW JERSEY INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9305344
• 关键词: Address; Adult; Affect; Anatomy; Animal Model; Apoptosis; Behavior; Behavioral; Biological Models; Brain; Calcium; Characteristics; Code; Communities; Complex; Development; Disease; Doctor of Philosophy; Elements; Embryo; Embryonic Development; Eye; Eye Development; Face; Fishes; Functional disorder; Future; Homeostasis; Image; Injury; Mammals; Measures; Modeling; Modification; Morphology; Natural regeneration; Nature; Neuroanatomy; Neurons; Ocular orbit; Outcome; Property; Recovery of Function; Research; Research Personnel; Research Training; Rest; Retina; Rivers; Schools; Scientist; Shapes; Signal Transduction; Structure; Students; Surface; System; Techniques; Tectum Mesencephali; Testing; Thinking; Training; Training Programs; Transplantation; Visual; Visual system structure; Zebrafish; base; behavioral study; blind; experience; extrastriate visual cortex; eye regeneration; high school; insight; lens; member; neuronal circuitry; novel; novel therapeutic intervention; relating to nervous system; response; superior colliculus Corpora quadrigemina; transmission process; undergraduate student; visual coding; visual information; volunteer

Summary Neuronal reorganization is a critical step in the recovery of function, whether after injury or as in response to disease. In our research training program, we will use a new animal model to address neural reorganization in the visual circuit. The fish Astyanax mexicanus provides a particularly novel window into network remodeling because it is extant in two readily available forms: a visual river-dwelling (surface fish) form and a cave-dwelling form (cavefish). It is closely related to zebrafish, and all techniques use in this zebrafish can be used in Astyanax. Adult cavefish lack eyes and the corresponding visual neural structures are severely reduced. Eye development is nevertheless initiated during embryogenesis, but later arrests with the lens undergoing programmed cell death and the rest of the eye degenerating and sinking into the orbit. However, it is possible to rescue the eye, and the corresponding visual circuits, by transplanting an embryonic surface fish lens into a degenerating cavefish eye. We will use neuroanatomy, behavior and calcium imaging to measure the changes that occur in the brains of blind cavefishes after developmental manipulation. Our central hypothesis is that the developing optic tectum circuitry in the cavefish is robust and receptive to coherent visual information, so that homeostatic mechanisms will allow for the emergence of visual codes. Our specific objective is to test the malleability of the optic tectum circuitry in response to visual input during development. To accomplish this, we will train two undergraduate students, one master's student and a PhD student as well as high school volunteers from a charter school in Newark, NJ.

总结

项目成果

Sensorimotor Transformations in the Zebrafish Auditory System.

斑马鱼听觉系统的感觉运动转变。

• DOI: 10.1016/j.cub.2019.10.020
• 发表时间: 2019
• 期刊: Current biology : CB
• 作者: Privat,Martin;Romano,SebastiánA;Pietri,Thomas;Jouary,Adrien;Boulanger-Weill,Jonathan;Elbaz,Nicolas;Duchemin,Auriane;Soares,Daphne;Sumbre,Germán
• 通讯作者: Sumbre,Germán

Evolutionary and homeostatic changes in morphology of visual dendrites of Mauthner cells in Astyanax blind cavefish.

• DOI: 10.1002/cne.25056
• 发表时间: 2021-06
• 期刊: The Journal of comparative neurology
• 作者: Tanvir Z;Rivera D;Severi KE;Haspel G;Soares D
• 通讯作者: Soares D

Morphological malleability of the lateral line allows for surface fish (Astyanax mexicanus) adaptation to cave environments.

侧线的形态可塑性使表层鱼类（Astyanax mexicanus）能够适应洞穴环境。

• DOI: 10.1002/jez.b.22953
• 发表时间: 2020
• 期刊: Journal of experimental zoology. Part B, Molecular and developmental evolution
• 作者: Yoffe,Marina;Patel,Kush;Palia,Eric;Kolawole,Samuel;Streets,Amy;Haspel,Gal;Soares,Daphne
• 通讯作者: Soares,Daphne