Discovery and Mechanism of Antimalarial Natural Products

抗疟天然产物的发现及其作用机制

• 批准号: 9272837
• 负责人: Maria Belen Cassera
• 金额: $ 39.41万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9272837
• 关键词: Africa South of the Sahara; Antimalarials; Artemisinins; Biochemical; Biological; Biological Assay; Chemicals; Chemistry; Chloroquine resistance; Collection; Combined Modality Therapy; Complementary and alternative medicine; Data; Development; Disabled Persons; Disease; Dose; Drug Combinations; Drug resistance; Evaluation; Falciparum Malaria; Fractionation; Future; Goals; Health; Human Cell Line; Institutes; Investigation; Lead; Libraries; Literature; Liver; Malaria; Mammalian Cell; Medical; Medical History; Metabolic; Molecular; Molecular Target; Natural Products; Natural Resources; Parasitic Diseases; Pharmaceutical Chemistry; Pharmaceutical Preparations; Pharmacologic Substance; Phenotype; Plant Extracts; Plant Taxonomy; Plants; Plasmodium falciparum; Proteomics; Quinine; Recording of previous events; Research; Resistance; Sampling; Selection Criteria; Series; Source; Specimen; Structure; Structure-Activity Relationship; Synthesis Chemistry; Therapeutic Intervention; Toxic effect; Virginia; Work; analog; asexual; base; biological systems; cytotoxicity; drug candidate; drug development; drug testing; improved; indexing; inhibitor/antagonist; metabolomics; novel; novel therapeutics; programs; public health relevance; repository; resistant strain; response; screening; transcriptomics

DESCRIPTION (provided by applicant): Malaria and other parasitic diseases are the greatest health problem currently facing the developing world, and P. falciparum malaria is a particularly severe problem in sub-Saharan Africa. Drug development is a necessary approach to reducing the worldwide impact of malaria, because it is the only approach that will benefit the millions of people currently afflicted with this disease. Natural products are a known, excellent source of antimalarial compounds. Two of the most effective antimalarial drugs (quinine and artemisinin) are natural products, and many synthetic antimalarial drugs are analogs of these two natural products. In addition to the use of isolated natural products as antimalarial agents, many plants are used ethno medically for the treatment of malaria. The development of new antimalarial natural products is however handicapped by a lack of understanding of their mechanism of action. This research will combine the antimalarial expertise of two research groups at Virginia Tech (VT) with the natural product resources of the Natural Products Discovery Institute (NPDI) in a collaborative program to tap into the enormous potential of natural products to serve as antimalarial agents. The NPDI maintains a repository of over 22,000 samples prepared from a total of approximately 7500 plant specimens. The antimalarial activity of a set of extracts from twelve plants in this collection with an ethno medical history of use as antimalarial agents has been validated at VT, and these extracts will be supplied by NPDI for isolation of novel antimalarial compounds from plants used in complementary and alternative medicine (CAM). In addition, all 22,000 extracts from the NPDI will be assayed for antimalarial activity by Dr. Belen Cassera at VT using a standard antimalarial bioassay to identify active extracts. Extracts which pass rigorous selection criteria will then be fractionated by Dr. David Kingston at VT, who will isolate and determine the structures of the active compounds from both the ethno medical extracts and active extracts found by screening the entire NPDI collection. Isolated compounds will be evaluated for stage specific activity (asexual intraerythrocytic stages, gametocytocidal, and liver stages), as well as cytocidal and anti-apicoplast activity to identify lead inhibitors wih different modes of action. The three most promising leads will then be selected to elucidate their mode of action and potential molecular target(s) using proteomics, metabolomics and transcriptomics approaches, with the ultimate goal of finding a novel antimalarial agent with a new mechanism of action. Synthetic chemistry will provide analogs of these lead compounds for future drug development.

描述（由申请人提供）：疟疾和其他寄生虫病是目前发展中国家面临的最大健康问题，恶性疟原虫疟疾是撒哈拉以南非洲地区特别严重的问题。药物开发是减少疟疾在全世界的影响的一个必要途径，因为这是使目前患有这种疾病的数百万人受益的唯一途径。天然产品是已知的抗疟化合物的优良来源。两种最有效的抗疟药物（奎宁和青蒿素）是天然产物，许多合成抗疟药物是这两种天然产物的类似物。除了使用分离的天然产品作为抗疟剂外，许多植物在民族医学上用于治疗疟疾。然而，由于对新的抗疟天然产品的作用机制缺乏了解，这些产品的开发受到阻碍。这项研究将联合收割机的抗疟专业知识的两个研究小组在弗吉尼亚理工大学（VT）与天然产品发现研究所（NPDI）的天然产品资源的合作计划，以挖掘天然产品作为抗疟剂的巨大潜力。NPDI保存了从总共约7500个植物标本中提取的22，000多个样本。在VT已经验证了来自该集合中的12种植物的一组提取物的抗疟活性，这些植物具有用作抗疟剂的民族医学史，这些提取物将由NPDI提供，用于从补充和替代医学（CAM）中使用的植物中分离新型抗疟化合物。此外，来自NPDI的所有22，000种提取物将由VT的Belen Cassera博士使用标准抗疟生物测定法进行抗疟活性测定，以鉴定活性提取物。通过严格选择标准的提取物将由VT的大卫金斯顿博士进行分级，他将从民族医学提取物和通过筛选整个NPDI收集物发现的活性提取物中分离并确定活性化合物的结构。将评价分离的化合物的阶段特异性活性（无性红细胞内阶段、杀配子和肝阶段）以及杀细胞和抗顶质体活性，以鉴定具有不同作用模式的先导抑制剂。然后将选择三个最有希望的线索，使用蛋白质组学，代谢组学和转录组学方法阐明其作用模式和潜在的分子靶点，最终目标是找到一种具有新作用机制的新型抗疟药。合成化学将为未来的药物开发提供这些先导化合物的类似物。