Ethanolamine Signaling in Virulence Gene Regulation and Host/Microbial Interactions

毒力基因调控和宿主/微生物相互作用中的乙醇胺信号传导

基本信息

  • 批准号:
    9262867
  • 负责人:
  • 金额:
    $ 38.89万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-05-11 至 2020-04-30
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): Escherichia coli O157:H7 (EHEC) is a food-borne pathogen that causes bloody diarrhea and hemolytic uremic syndrome (HUS), a complication of EHEC disease that can lead to kidney failure, throughout the world. EHEC has a very low infectious dose, making it difficult to control epidemiologically. EHEC colonizes the large intestine where it attaches to colonic epithelial cells and causes effacement of microvilli. EHEC also produces Shiga toxin that causes the major symptoms of HUS. In order to successfully colonize the host and establish infection, EHEC must be able to compete with the indigenous microbiota for nutrients. Ethanolamine (EA) is present in the gastrointestinal tract. EHEC uses EA as a nitrogen source, thereby gaining a competitive advantage over the indigenous micriobiota. Importantly, EHEC has co-opted EA as a signal to promote expression of genes involved in host colonization and infection as well as in Shiga toxin production. The transcriptional regulator EutR activates expression of genes that code for EA metabolism, and we determined that EutR promotes expression of EHEC virulence genes. Although EutR is important for EHEC pathogenesis, very little is known concerning the extent of EutR gene regulation in EHEC or how EutR functions to regulate virulence genes. In Specific Aim 1, we will perform a detailed characterization of the role of EutR in EHEC pathogenesis as well as examine the mechanisms of EutR virulence gene regulation. Interestingly, EHEC responds to EA and modulates virulence gene expression in the eutR mutant strain suggesting that EHEC encodes a second EA sensor. In Specific Aim 2, we will investigate the roles and mechanisms of EA-dependent, EutR-independent virulence gene regulation in EHEC. Specific Aim 3 will address how these EA-sensors contribute to EHEC fitness and virulence gene expression during infection. The proposed experimental approaches will achieve a better understanding of mechanisms used by EHEC to activate its virulence genes and may reveal novel aspects of EHEC pathogenesis. Altogether, these data may lead to the development of unique strategies for intervention and/or treatment for EHEC disease.


项目成果

期刊论文数量(0)
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Melissa Kendall其他文献

Melissa Kendall的其他文献

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{{ truncateString('Melissa Kendall', 18)}}的其他基金

RNA-binding proteins in bacterial virulence and host-pathogen interactions
RNA结合蛋白在细菌毒力和宿主-病原体相互作用中的作用
  • 批准号:
    10659346
  • 财政年份:
    2023
  • 资助金额:
    $ 38.89万
  • 项目类别:
sRNAs in EHEC virulence
EHEC毒力中的sRNA
  • 批准号:
    10408173
  • 财政年份:
    2021
  • 资助金额:
    $ 38.89万
  • 项目类别:
sRNAs in EHEC virulence
EHEC毒力中的sRNA
  • 批准号:
    10286819
  • 财政年份:
    2021
  • 资助金额:
    $ 38.89万
  • 项目类别:
RNA helicases in bacterial pathogenesis
RNA解旋酶在细菌发病机制中的作用
  • 批准号:
    10217792
  • 财政年份:
    2021
  • 资助金额:
    $ 38.89万
  • 项目类别:
RNA helicases in bacterial pathogenesis
RNA解旋酶在细菌发病机制中的作用
  • 批准号:
    10360660
  • 财政年份:
    2021
  • 资助金额:
    $ 38.89万
  • 项目类别:
Roles of Archaea in infectious disease
古细菌在传染病中的作用
  • 批准号:
    9808070
  • 财政年份:
    2019
  • 资助金额:
    $ 38.89万
  • 项目类别:
Ethanolamine Signaling in Virulence Gene Regulation and Host/Microbial Interactions
毒力基因调控和宿主/微生物相互作用中的乙醇胺信号传导
  • 批准号:
    9065488
  • 财政年份:
    2015
  • 资助金额:
    $ 38.89万
  • 项目类别:
Regulation of EHEC virulence genes by QseA and small RNA molecules
QseA 和小 RNA 分子对 EHEC 毒力基因的调控
  • 批准号:
    7545685
  • 财政年份:
    2008
  • 资助金额:
    $ 38.89万
  • 项目类别:
Regulation of EHEC virulence genes by QseA and small RNA molecules
QseA 和小 RNA 分子对 EHEC 毒力基因的调控
  • 批准号:
    7807910
  • 财政年份:
    2008
  • 资助金额:
    $ 38.89万
  • 项目类别:

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