Core A - Bioinformatics Core

核心 A - 生物信息学核心

• 批准号: 9209392
• 负责人: JEFFREY P. BOND
• 金额: $ 11.48万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-03 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9209392
• 关键词: Address; Base Excision Repairs; Biochemical; Biochemistry; Bioinformatics; Biological; Biological Models; Catalysis; Cell Proliferation; Cell Survival; Cell model; Cellular biology; Clinical; Collection; Communication; Complex; Computer software; DNA Damage; DNA Repair; DNA Repair Enzymes; DNA Repair Gene; DNA Sequence; DNA Sequence Alteration; Data; Data Sources; Databases; Defect; Development; Documentation; Enzyme Kinetics; Equipment and supply inventories; Evaluation; Event; Experimental Designs; Frequencies; Genes; Genetic Variation; Genome; Genomics; Goals; Health; High Performance Computing; Human; Human Genetics; Human Resources; Informatics; Knowledge; Laboratories; Laboratory Study; Large-Scale Sequencing; Link; Logistics; Malignant Neoplasms; Manuals; Medicine; Modeling; Molecular; Molecular Biology; Mus; OGG1 gene; Oncogenes; POLB gene; Pathway interactions; Phenotype; Population; Positioning Attribute; Post-Translational Protein Processing; Pre-Clinical Model; Process; Program Research Project Grants; Proteins; Publications; Reporting; Research Infrastructure; Role; Sampling; Service Statistics; Services; Somatic Mutation; Statistical Data Interpretation; Structure; System; Systems Biology; Testing; The Cancer Genome Atlas; Therapeutic; Time; Transgenic Mice; Variant; Work; XRCC1 gene; base; cancer therapy; carcinogenesis; chemotherapy; computerized tools; data management; design; experimental analysis; experimental study; meetings; member; molecular sequence database; multidisciplinary; novel; predicting response; predictive marker; programs; protein function; protein structure; repair enzyme; single molecule; statistical service; statistics; synergism; tumor

SUMMARY – CORE A The four research projects of the Program Project propose laboratory characterization of the consequences of genetic variation for carcinogenesis and cancer therapy. These studies are focused on genes involved in Base Excision Repair (BER). Genetic variation will include germline and somatic variation. Core A will provide informatics and statistics support for these experiments. Core A personnel have expertise in bioinformatics, systems biology, statistics, process management, and high performance computing. Dr. Bond has worked with the P01 since its inception. Aim 1 (Informatics) Identify variants for functional studies. A central role of Core A is to support identification of variants, for experimental studies, that are expected to have consequences for protein function. These variants are identified using DNA sequences available from large- scale sequence projects such as the 1000 Genomes Project and The Cancer Genome Atlas. Core A annotates the variants using publicly available software. Core A (Drs. Bond and Dragon) host weekly meetings at which these variants are presented and discussed. These meetings provide consistent communications with members of the four projects. Aim 2 (Statistics) Provide software and statistical support for hypothesis tests. The laboratory studies proposed in Projects 1-4 will characterize the consequences of this variation for protein structure, interactions, damage search and catalysis as well as cellular proliferation and survival. Core A statistical services will support design and analysis of these experiments.

摘要-核心A 该计划项目的四个研究项目提出了实验室表征的后果， 遗传变异的致癌作用和癌症治疗。这些研究集中在与碱基有关的基因上 切除修复术（BER）。遗传变异将包括种系和体细胞变异。核心A将提供 信息学和统计学支持这些实验。核心A人员具有生物信息学方面的专业知识， 系统生物学、统计学、过程管理和高性能计算。邦德博士曾与 P01自成立以来目标1（信息学）识别功能研究的变体。的中心作用 核心A是支持鉴定变异，用于实验研究，预期具有 对蛋白质功能的影响。这些变体是使用可从大规模的DNA序列鉴定的， 大规模测序项目，如1000个基因组计划和癌症基因组图谱。芯A 使用公开可用的软件注释变体。核心A（邦德博士和龙博士）主持每周会议 在那里，这些变量被提出和讨论。这些会议提供了一致的沟通， 四个项目的成员。目标2（统计学）为假设提供软件和统计支持 试验.项目1-4中提议的实验室研究将描述这种变化的后果， 蛋白质结构、相互作用、损伤搜索和催化以及细胞增殖和存活。核心 统计服务将支持这些实验的设计和分析。