Genome Technologies and Bioinformatics

基因组技术和生物信息学

• 批准号: 10006838
• 负责人: JEFFREY P. BOND
• 金额: $ 23.38万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-05 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10006838
• 关键词: Applications Grants; Archives; Back; Bioinformatics; Biomedical Research; Centers of Research Excellence; Characteristics; Clostridium difficile; Collaborations; Communicable Diseases; Communities; Core Facility; DNA sequencing; Data; Data Analyses; Data Collection; Databases; Development; Documentation; Education; Educational workshop; Epigenetic Process; Equipment; Experimental Designs; Faculty; Fee-for-Service Plans; Gene Chips; Gene Expression; Genes; Genome; Genomics; Goals; Histones; Human Resources; Immunobiology; Immunology; Infrastructure; Institution; Laboratories; Leadership; Libraries; Linear Models; Manuscripts; Massive Parallel Sequencing; Measurement; Medicine; Methodology; Methylation; Modification; Multivariate Analysis; Oligonucleotide Microarrays; Persons; Phase; Process; Pseudomonas aeruginosa; Publications; RNA; Research; Research Personnel; Review Committee; Services; Stream; Structure; Surveys; Techniques; Technology; TimeLine; Universities; Variant; Vermont; Vocabulary; base; college; cost; data acquisition; data submission; deep sequencing; design; exome; exome sequencing; experience; experimental study; falls; genome sequencing; genome-wide; genomic data; instrumentation; member; microbial; pathogenic bacteria; programs; reference genome; sequencing platform; statistics; success; transcriptome sequencing

SUMMARY for the Genome Technologies and Bioinformatics Core The uniformly recognized significance of genomics and bioinformatics for the biomedical research community is also reflected in needs expressed by VCIID faculty members. This core provides support in all aspects of genomics, from the design stage, through data acquisition/quality assessment, and finally extensive bioinformatics support. The core uses an Affymetrix GeneChip and Illumina HiSeq technologies for oligonucleotide arrays and massively parallel sequencing, respectively. It is currently supporting projects for 11 COBRE faculty members, and has performed genomics data collection and analysis for 86 active COBRE projects. Preliminary discussions are underway for new projects with 16 additional COBRE faculty members. Accomplishments from Phases I and II include support for 28 publications, and addition of massively parallel sequencing services. Based on over 40 person-years of experience among the Core Directors leading core facilities, they identified eight essential characteristics needed for the Core: qualified and experienced staff, state-of-the-art equipment, education, responsiveness, process, experiment design, quality assessment, and sustainability. The Phase III COBRE objectives of the Genome Technologies and Bioinformatics Core satisfy these essential characteristics. Specific Aim 1 will provide state-of-the-art equipment, cutting-edge techniques, and experienced personnel to enhance the research of the VCIID-COBRE. Specific Aim 2 will provide a wide array of services to VCIID-COBRE faculty to facilitate their research. We will meet this goal by applying our standard process to projects that includes oversight by a project manager, experiment design, defined vocabulary, quality assessment, archival storage, and documentation. We have surveyed the user base, and are prepared to offer efficient and effective service to support VCIID collaborations. Specific Aim 3 will continue our Phase II plan for sustainability that includes a greatly expanded user base, both at UVM as well as at several other campuses. We have developed a progressive fee-for-service structure, and are highly responsive to the need for constant adaptation to changes in research needs. We have received strong commitments for sustained support from the institution for at least five years beyond Phase III, and we will add revenue streams based on VCIID collaborations that will sustain our core beyond Phase III. Our aims and objectives reflect both the eight essential characteristics identified by our leadership and the VCIID needs identified by a survey of the VCIID. These aims will facilitate the transition of the Core into a fully self-sustainable facility capable of continuing to support state-of-the-art research.

基因组技术和生物信息学核心概要 基因组学和生物信息学对生物医学研究界的一致认可的重要性 也反映在VCIID教职员工表达的需求中。该核心在以下各方面提供支持： 基因组学，从设计阶段，通过数据采集/质量评估，最后广泛 生物信息学支持。该核心使用Affymetrix GeneChip和Illumina HiSeq技术， 寡核苷酸阵列和大规模平行测序。它目前正在支持以下项目： 11名COBRE教职员工，并为86名活跃的COBRE进行了基因组学数据收集和分析 项目正在与另外16名COBRE教员就新项目进行初步讨论。 第一阶段和第二阶段的成就包括支持28个出版物，以及增加大规模并行 测序服务。 根据核心董事领导核心设施的40多人年经验，他们确定 核心所需的八个基本特征：合格和经验丰富的工作人员，最先进的设备， 教育、反应能力、过程、实验设计、质量评估和可持续性。III期 基因组技术和生物信息学核心的COBRE目标满足这些基本要求 特色具体目标1将提供最先进的设备，尖端技术， 经验丰富的人员，以加强研究的VCIIID-COBRE。具体目标2将提供广泛的 一系列的服务，以VCIID-COBRE教师，以促进他们的研究。我们将通过应用我们的 项目的标准流程，包括项目经理的监督、实验设计、定义 词汇、质量评估、档案存储和文档。我们调查了用户群， 准备提供高效和有效的服务，以支持VCIID的合作。第3章将 继续我们的第二阶段可持续发展计划，包括大大扩大用户群，无论是在UVM以及 和其他几个校区一样我们已经制定了一个渐进的收费服务结构，并高度 不断适应研究需求的变化。我们收到了强烈的 承诺在第三阶段之后至少五年内得到该机构的持续支持， 基于VCIID合作的收入流，将在第三阶段之后维持我们的核心。 我们的宗旨和目标既反映了我们领导层确定的八个基本特征， 通过对《维也纳宣言和行动纲领》的调查确定《维也纳宣言和行动纲领》的需要。这些目标将促进核心小组过渡到一个全面的 自我维持的设施，能够继续支持最先进的研究。