CORE--BIOINFORMATICS CORE

该计划项目的总体目标是在原子水平上了解三个DNA修复酶家族如何识别和加工其底物。生物信息学核心的中心目标是利用序列，结构和功能数据设计和解释实验，以实现计划项目的目标。研究项目的目的包括使用功能测量和X射线晶体学来测试关于天然酶和这些酶的定点突变体的假设。生物信息学核心将提供工具，利用这些家族的结构和功能的现有知识，为这些研究鉴定天然酶和定点突变体。结果 生物信息学核心将使用这些实验工作来设计后续实验。 生物信息学核心的目标是： 目标1：为信息管理和分析提供基础设施。该核心将提供稳定和安全的信息管理以及灵活的查询机制和广泛的生物信息学工具。这些信息将包括原始数据、实验设计和推断结果。 分析工具将包括现有的应用程序和提高研究生产力的定制分析工具。 目的2：建立基于系统发育树的天然酶筛选方法，用于功能和结构研究。 目的3：建立基于系统发育树的天然酶定点突变体筛选方法，用于功能和结构研究。

The overall goal of this Program Project is to understand at the atomic level how three families of DNA repair enzymes recognize and process their substrates. The central goal of the Bioinformatics Core is to make use of sequence, structure, and function data for the design and interpretation of experiments that will achieve the goal of the Program Project. The Aims of the Research Projects involve testing hypotheses about natural enzymes and site-directed mutants of these enzymes using functional measurements and X-ray crystallography. The Bioinformatics Core will provide tools that use existing knowledge of the structure and function of these families to identify natural enzymes and site-directed mutants for these studies. The results of the experimental work will be used by the Bioinformatics Core for designing subsequent experiments. The Aims of the Bioinformatics Core are: Aim 1: To provide infrastructure for information management and analysis. The Core will provide stable and secure information management as well as flexible querying mechanisms and a broad spectrum of bioinformatics tools. The information will include primary data, experimental designs, and inferred results. The analysis tools will include existing applications and custom analysis tools that enhance research productivity. Aim 2: To develop phylogenetic tree-based methods for selecting natural enzymes for functional and structural studies. Aim 3: To develop phylogenetic tree-based methods for selecting site-directed mutants of natural enzymes for functional and structural studies.