Enhancing Capability and Capacity of Maryland’s Laboratory to Conduct Surveillance of Antibiotic Resistance Pathogens in Selected Retail Foods

增强马里兰州实验室对选定零售食品中抗生素耐药性病原体进行监测的能力

• 批准号: 9336712
• 负责人: pongpan Laksanalamai
• 金额: $ 12.55万
• 依托单位: MARYLAND STATE DEPARTMENT OF HEALTH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9336712
• 关键词: Enhancing; Capability; Capacity; Maryland; Laboratory

Abstract Food-borne bacterial infections contribute to morbidity and mortality world-wide. The National Antimicrobial Resistance Monitoring System (NARMS) was established to perform public health surveillance in the United States to track antimicrobial resistance in enteric bacteria isolated from retails food products. The MD Department of Health and Mental Hygiene has been participating in the NARMS program for numbers of years and successfully contributed to the development of the data used in the studies of bacterial illness to retail food contamination. The study has been performed in collaboration between the State of Maryland DHMH epidemiologists and laboratory scientists to assure the quality of the results. Retail foods including ground beef, pork chops, ground turkey and chicken breast with skin, are purchased by the MD DHMH staff from stores the Baltimore metropolitan areas every two weeks. The samples are processed according to the protocols for the presence of Salmonella, E. coli and Enterococci. The poultry samples are also tested for the presence of Campylobacter. Bacterial isolate’s identity are then confirmed with the appropriate methods. Additionally, we are capable of serotyping Salmonella isolates and speciating the other isolates using molecular methods. After all isolates are confirmed and frozen at -80C, they are submitted to the FDA for antibiotic susceptibility testing and further characterization.

摘要 食源性细菌感染导致世界范围内的发病率和死亡率。国家 建立了抗菌药物耐药性监测系统（NARMS）， 在美国进行健康监测，以追踪肠道细菌的抗菌素耐药性 从零售食品中分离出来。医学博士健康和心理卫生部一直在 多年来参与NARMS计划，并成功地为 发展用于细菌性疾病到零售食品污染研究的数据。的 本研究是与马里兰州DHMH合作进行的 流行病学家和实验室科学家确保结果的质量。零售食品 包括碎牛肉、猪排、碎火鸡肉和带皮鸡胸肉， MD DHMH的员工每两周从巴尔的摩大都市区的商店。的 根据沙门氏菌、E.杆菌和 肠球菌家禽样品也被测试弯曲杆菌的存在。细菌 然后用适当的方法确认分离物的身份。此外，我们有能力 对沙门氏菌分离株进行血清分型，并使用分子方法对其他分离株进行分类。 在所有分离株被确认并在-80 ° C冷冻后，它们被提交给FDA进行抗生素治疗。 敏感性测试和进一步表征。