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Contribution of LiaFSR to pathogenesis and carriage of group A Streptococcus

LiaFSR 对 A 族链球菌发病机制和携带的贡献

基本信息

批准号：
9605689
负责人：
Anthony Richard Flores
金额：
$ 38.5万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-01-16 至 2021-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9605689
关键词：
Adherence Affect Amino Acids Antibiotics Arginine Bacteria Benign Biological Assay Carrier State Cell Wall ChIP-seq Detection Diagnostic Disease Eating Event Firefly Luciferases Foundations Future Gene Expression Regulation Gene Targeting Genes Genetic Determinism Glean Glycine Goals Gram-Positive Bacteria Growth High-Throughput Nucleotide Sequencing Human In Vitro Infection Investigation Lead Learning Light Molecular Genetics Morbidity - disease rate Mucous Membrane Mutation Necrotizing fasciitis Pathogenesis Pharyngeal structure Pharyngitis Phenotype Phosphoric Monoester Hydrolases Phosphorylation Phosphotransferases Plasma Positioning Attribute Regulator Genes Regulon Reporter Research Saliva Serotyping Signal Transduction Streptococcus pyogenes Surface Symptoms System Testing Time Virulence antimicrobial peptide base chromatin immunoprecipitation in vivo Model mortality mutant mutation carrier pathogen pathogenic bacteria response sensor therapeutic development transcriptome transcriptome sequencing vaccine development

项目摘要

Contribution of LiaFSR to pathogenesis and carriage of group A Streptococcus PROJECT SUMMARY Asymptomatic carriage of bacterial pathogens is a common but poorly understood phenomenon. Group A Streptococcus (GAS) is the cause of severe infections such as the “flesh-eating” necrotizing fasciitis and more benign infections like pharyngitis. However, GAS is also carried in the throats of humans in the absence of symptoms (i.e. asymptomatic carriage). We understand little of the bacterial molecular genetic determinants contributing to GAS carriage. We hypothesize that mutations in GAS carrier strains contribute to a unique phenotype characterized by increased ability to adhere to mucosal surfaces and decreased ability to cause disease. We identified a mutation, unique to a GAS carrier strain, leading to a single amino acid change in a highly conserved sensor kinase, LiaS, of the poorly described GAS regulatory three component system (3CS) LiaFSR. We previously showed complete recapitulation of the carrier phenotype (increased adherence to mucosal surfaces and decreased virulence) when the carrier mutation in liaS was introduced into a serotype- matched disease-causing strain of GAS. We also discovered that the carrier mutation altered global gene regulation. The LiaFSR 3CS has been described in other bacteria but its contribution to virulence gene regulation in pathogenic bacteria is largely unknown. Further, the mechanism by which the carrier amino acid change in LiaS leads to gene dysregulation and the carrier phenotype is incomplete. We hypothesize that the carrier mutation alters function of the sensor kinase, LiaS, resulting in decreased activation of the target response regulator, LiaR, affecting gene regulation and leading to the carrier phenotype. By defining the mechanism by which the carrier mutation alters gene regulation and leads to a carrier phenotype, we may better understand the gene regulatory events associated with the transition from disease to carriage in GAS. We have proposed three aims to test our hypothesis. In Aim 1, we will define in vitro and ex vivo-human conditions that activate the LiaFSR system. Aim 2 will determine global gene regulation by LiaFSR in GAS and define the genes affected by the carrier LiaS mutation. In Aim 3, we will demonstrate the effect of the carrier mutation on the function of LiaS and subsequent response regulator activation. Upon completion of these aims, we will have defined the mechanism by which the carrier mutation leads to gene dysregulation and the carrier phenotype and simultaneously learned more about gene regulation in GAS pathogenesis. Ultimately, these studies will provide a foundation for future carriage research and potentially have implications in diagnostic, therapeutic, and vaccine development for GAS and other bacterial pathogens.

LiaFSR在A群链球菌发病和携带中的作用