Resource Core

资源核心

• 批准号: 9536845
• 负责人: OLIVIER LE SAUX
• 金额: $ 63.97万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9536845
• 关键词: Address; Affect; Animal Model; Animals; Bioinformatics; Biomedical Research; Caucasians; Cell model; Cells; Centers of Research Excellence; Clinical; Communities; Data Analyses; Data Quality; Development; Diabetes Mellitus; Diagnosis; Disease; Education; Ensure; Environment; Epidemic; Epigenetic Process; Fee-for-Service Plans; Filipino; Gene Expression; Genes; Genetic Transcription; Genetic Variation; Genome; Genomic DNA; Genomics; Genus Hippocampus; Goals; Grant; Hawaii; Hawaiian population; Health; Heritability; Hyperglycemia; In Vitro; Instruction; International; Ions; Island; Japanese Population; Libraries; Mentors; Metabolic; Metabolism; Minority; Modernization; Mutation; Pacific Island Americans; Population; Population Group; Prevention; Recovery; Research; Research Infrastructure; Research Personnel; Resources; Services; Site; Technology; Training; Training and Education; Universities; Variant; Work; biomedical resource; clinical Diagnosis; cost; design; epigenomics; health disparity; improved; in vivo; instrumentation; interest; metabolic abnormality assessment; new technology; next generation; transcriptome sequencing

Abstract: The overall objective of the Resource Core is to provide an easily accessible, modern, and local set of expertise in cellular and animal metabolism and the epigenetics associated with diabetes to ultimately provide a sustainable resource for the biomedical research community to assess cellular and animal metabolics along with the genetic changes associated with a clinical diagnosis. The central hypothesis for this Resource Core is that the core will provide an unprecedented set of expertise, education, and training by establishing the infrastructure necessary to investigate the mechanisms, diagnosis, prevention, and/or treatment of diabetes in a multi-faceted approach to improve health and address health disparities in minority populations in Hawaii. The following aims will support the goals of the Resource Core to achieve the overall objective. Specific Aim 1: Establish a cell and animal metabolic facility for the growing number of investigators interested in studying diabetes. We plan to enhance cellular metabolic instrumentation and install animal metabolic infrastructure to study diabetes. We will provide services in, in-vitro to in-vivo metabolic studies including the design, development, optimization, and delivery as requested, as we have extensive expertise in cellular metabolism and animal models. Specific Aim 2: Establish an epigenetics facility to evaluate heritable variations and transcriptional changes in genes associated with diabetes. We have extensive expertise in gene expression studies to develop and deliver a wide-array of epigenomic services. We plan to enhance the epigenetics core by providing expertise and ground-breaking technology like MeDIP-, ChIP-, gDNA-, Ampli-, and RNA- sequencing and bioinformatics data analysis. This technology will be accessible to interested investigators. Specific Aim 3: Provide education and instruction in the resources and capabilities of the Core. Cores are critical to produce high quality data but also to the training and development of “quality” investigators. The advantage of a core is the consistency and quality of the work performed. Specifically, this core will include training and facilitation of use to assess cellular metabolism (Seahorse XFe96), animal metabolism (GTT, ITT, hyperglycemic and hyperinsulinemic-euglycemic), and genetic variation (Ion Personal Genome Machine®). We plan to integrate a fee for service/cost recovery/sharing plan to ensure that the core is self-sustaining while integrating new technologies in the field.

摘要： 资源核心的总体目标是提供一套易于获取的、现代的和本地的 细胞和动物代谢以及与糖尿病相关的表观遗传学方面的专业知识， 生物医学研究界评估细胞和动物代谢的可持续资源，沿着 与临床诊断相关的基因变化。此资源核心的中心假设 核心将提供一套前所未有的专业知识，教育和培训， 研究糖尿病的机制、诊断、预防和/或治疗所需的基础设施， 一个多方面的方法，以改善健康和解决健康差距在少数民族人口在夏威夷。 以下目标将支持资源核心的各项目标，以实现总体目标。 具体目标1：为越来越多的研究人员建立细胞和动物代谢设施 对研究糖尿病感兴趣。 我们计划加强细胞代谢仪器和安装动物代谢基础设施， 糖尿病我们将提供从体外到体内代谢研究的服务，包括设计，开发， 优化，并根据要求交付，因为我们在细胞代谢和动物 模型 具体目标2：建立表观遗传学设施，以评估遗传变异和转录 与糖尿病相关的基因变化。 我们在基因表达研究方面拥有丰富的专业知识，可以开发和提供广泛的表观基因组 服务我们计划通过提供专业知识和突破性技术， MeDIP、ChIP、gDNA、Ampli和RNA测序和生物信息学数据分析。这项技术将 感兴趣的调查人员可以访问。 具体目标3：提供关于核心资源和能力的教育和指导。 核心对于产生高质量数据至关重要，但对于“质量”的培训和发展也至关重要。 investigators.核心的优势在于所执行工作的一致性和质量。具体地说， 该核心将包括用于评估细胞代谢的培训和促进（Seahorse XFe 96）， 动物代谢（GTT、ITT、高血糖和高胰岛素血症-正常血糖）和遗传变异 (Ion Personal Genome Machine®）。我们计划整合服务收费/成本回收/分担计划， 确保核心能够自我维持，同时在实地采用新技术。