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Visualizing endogenous ion channel activation

内源性离子通道激活的可视化

基本信息

批准号：
9750842
负责人：
Bruce E Cohen
金额：
$ 35.9万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-30 至 2021-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9750842
关键词：
Action Potentials Address Amino Acids Binding Cells Characteristics Charge Collection Color Complement Complex Dissociation Drug Targeting Electrophysiology (science)Electrostatics Engineering Environment Exhibits Fluorescence Fluorescent Probes Gated Ion Channel Goals Hippocampus (Brain)Human Hyperactive behavior Image Individual Ion Channel Ion Channel Gating Ischemia Kinetics Ligands Measurement Measures Membrane Modeling Molecular Molecular Conformation Monitor Nervous system structure Neurons Optical reporter Optics Pathologic Pattern Peptides Physiological Play Positioning Attribute Primates Probability Protocols documentation Quantum Dots Reporting Role Scanning Signal Transduction Spiders Spinal Ganglia Structure Surface Technology Testing Therapeutic Thermodynamics Tissue imaging Tissues Toxin Validation base chronic itch design fluorescence imaging fluorophore genetic manipulation imaging probe lipophilicity neuronal circuitry novel novel therapeutics painful neuropathy prototype quantitative imaging relating to nervous system response sensor spectrograph tool voltage

项目摘要

The expansive goal of this project is to develop new means to visualize the activation of endogenous voltage gated ion channels (VGICs). Difficulties in identifying when and where specific VGICs become involved in electrical signals limit our fundamental understanding of neuronal circuits and impede the discovery of therapeutics. Novel tools to image the activity of VGIC subtypes enable fluorescence imaging of channel participation in physiological and pathophysiological signaling. Technology to see specific ion channels activate could aid in validation of drug targets for a host of maladies, including chronic itch and neuropathic pain. Neurons exhibit tremendous diversity in their electrical signaling, a consequence of the distinct collections of VGICs different cells. Understanding how individual VGIC subtypes contribute to electrical signaling remains a significant challenge. We propose to develop ion channel activity probes to monitor activity of VGICs in neurons. This technology we are developing can, in principle, be used to target any subtype of VGIC.

这个项目的扩展目标是开发新的方法来可视化激活内源性电压门控离子通道(VGIC)。难以确定时间和时间当特定的VGIC参与电信号时，限制了我们的基本原理对神经元回路的理解，阻碍了治疗学的发现。小说 VGIC亚型活动成像工具可实现通道的荧光成像参与生理和病理生理信号。值得关注的技术激活特定的离子通道可以帮助确认一系列药物靶点不适，包括慢性瘙痒和神经病理性疼痛。神经元表现出巨大的它们电信号的多样性，这是VGIC不同集合的结果不同的细胞。了解各个VGIC亚型如何影响电气信号仍然是一个巨大的挑战。我们建议开发离子通道活性用于监测神经元中VGICs活性的探针。我们正在开发的技术可以，原则上，可用于VGIC的任何亚型。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Azide-Alkyne Click Conjugation on Quantum Dots by Selective Copper Coordination.

DOI：
10.1021/acsnano.8b00575
发表时间：
2018-05-22
期刊：
ACS nano
影响因子：
17.1
作者：
Mann VR;Powers AS;Tilley DC;Sack JT;Cohen BE
通讯作者：
Cohen BE

Photostable and efficient upconverting nanocrystal-based chemical sensors.

DOI：
10.1016/j.optmat.2018.07.031
发表时间：
2018-10
期刊：
Optical Materials
影响因子：
3.9
作者：
Cheryl A. Tajon;Hao Yang;Bining Tian;Yue Tian;P. Ercius;P. Schuck;E. Chan;B. Cohen
通讯作者：
Cheryl A. Tajon;Hao Yang;Bining Tian;Yue Tian;P. Ercius;P. Schuck;E. Chan;B. Cohen

Distinct cellular expression and subcellular localization of Kv2 voltage-gated K+ channel subtypes in dorsal root ganglion neurons conserved between mice and humans.

小鼠和人类之间保守的背根神经节神经元中 Kv2 电压门控 K 通道亚型的独特细胞表达和亚细胞定位。