Completely biological tissue-engineered pulmonic valve grown in vitro from human cells for pediatric patients

完全生物组织工程肺动脉瓣，由人体细胞在体外培养，供儿科患者使用

• 批准号: 9520733
• 负责人: ROBERT T TRANQUILLO
• 金额: $ 70.64万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9520733
• 关键词: Address; Adolescent; Adult; Animal Model; Area; Biochemical; Biological; Biological Assay; Caliber; Cardiac Surgery procedures; Cells; Cellularity; Child; Childhood; Clinical; Code; Commissure; Computer-Aided Design; Consensus; Data; Defect; Dimensions; Drops; Elements; Engineering; Ensure; Face; Failure; Fibroblasts; Funding; Geometry; Goals; Growth; Harvest; Heart Valves; Histology; Human; Immunohistochemistry; Implant; In Situ; In Vitro; Length; Liquid substance; Longitudinal Studies; Lung; Measurement; Measures; Modeling; Monitor; Outcomes Research; Patients; Performance; Phenotype; Physiologic pulse; Plant Roots; Positioning Attribute; Pre-Clinical Model; Preclinical Testing; Pulmonary artery structure; Pulmonary valve structure; Sheep; Site; Skin; Solid; Sterility; Stress; Structure; Surface; Surgical sutures; System; Systolic Pressure; Testing; Time; Tissue Engineering; Tissues; Translations; Tube; Ultrasonography; Validation; aortic valve; aortic valve disorder; aortic valve replacement; base; cardiac tissue engineering; clinically relevant; conditioning; design; experience; hemodynamics; implantation; mechanical properties; novel; pediatric patients; pressure; shear stress; success

Abstract The goal of this project is to create a heart valve that can grow with children and never require replacement. Using a biologically-engineered collagenous matrix in the form of a tube that has been shown to grow as a pulmonary artery replacement in lambs, and a novel design for a heart valve made from these tubes and degradable suture that confers durable commissures as well as valve growth potential, we will design, optimize, test, and implant tri-tube valves in a juvenile sheep model to demonstrate valve growth. Aim 1 will use computer-aided design (a fluid-structure interaction model for valve opening and finite element model for the closed valve state) to screen combinations of design parameters (defining coaptation area and geometry) for efficient valve function, Aim 2 will test the optimal designs in a pulse duplicator and validate/refine the CAD models, and Aim 3 will involve studies of the valve in growing juvenile sheep. Long-term studies of pulmonary and aortic valve replacement are designed with measurements to rigorously address valve growth.

摘要