Investigating The Cardiovascular Toxicity Of Exposure To Electronic Hookah Smoking

研究电子水烟对心血管的毒性

• 批准号: 9647995
• 负责人: Mary Rezk-Hanna
• 金额: $ 21.93万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9647995
• 关键词: Acute; Adult; Affect; Air Pollution; Anti-inflammatory; Antioxidants; Aorta; Arylesterase; Ascorbic Acid; Atherosclerosis; Attention; Attenuated; Award; Belief; Biological Markers; Blood Pressure; Blood Vessels; Buffers; Caliber; Carbon; Carbon Monoxide; Cardiac; Cardiovascular Diseases; Cardiovascular system; Charcoal; Cigarette; Cross-Over Studies; Deterioration; Development; Electronic Nicotine Delivery Systems; Electronic cigarette; Epidemic; Exposure to; Female; Flavoring; Future; Glycerol; Goals; Heating; High Density Lipoproteins; Human; IL8 gene; Impairment; Inflammation; Inflammatory; Infusion procedures; Inhalation; Interleukin-10; Interleukin-6; Intravenous; Lipoproteins; Liquid substance; Literature; Marketing; Measures; Mediating; Mediation; Methodology; Nicotine; Oral cavity; Oxidative Stress; Oxides; Pathogenesis; Physiologic pulse; Physiological; Plasma; Play; Policies; Process; Propylene Glycols; Regulation; Risk; Risk Factors; Role; Smoke; Smoker; Smoking; TNF gene; Tars; Testing; Tobacco; Toxic effect; Toxicant exposure; Toxin; Translational Research; United States; Vasodilation; Vasodilator Agents; Water; Youth; aerosolized; arterial stiffness; base; brachial artery; combustion product; comparative; cytokine; electronic hookah; fine particles; hookah; indexing; pipe smoking; pressure; prevent; research study; tobacco regulatory science; tobacco toxicant; vapor; virtual; young adult

Abstract Hookah (water-pipe) smoking is rapidly increasing in popularity worldwide. Contributing to this popularity is the unsubstantiated belief that traditional hookah smoke is detoxified as it passes through the water-pipe. More recently, electronic hookah bowls—containing e-liquid that is heated electrically but inhaled through traditional water-pipes—are increasing in popularity in the United States among young female adults, who endorse marketing claims that these products are even safer than traditional hookah. With traditional hookah, in addition to nicotine and tar, smokers are exposed to carbon-enriched fine particles and significant amounts of carbon monoxide (CO), the latter of which has been shown to constitute an endothelial-dependent vasodilator. Though toxicant exposure is much lower than traditional hookah and without any CO exposure, e-hookah bowls deliver nicotine and fine particles that constitute putative vascular toxins. The main objective of this project is to investigate the comparative effects of traditional hookah smoking versus electronic hookah bowl inhalation on endothelial and vascular function and their mechanistic role in the development of cardiovascular disease. Specifically, we will examine the role of oxidative stress and inflammation as potential mechanisms of action in hookah-induced cardiovascular disease. We will test the hypothesis that; 1) in the absence of charcoal briquettes and virtually any CO exposure, e-hookah bowl inhalation acutely impairs endothelial function and evokes acute central stiffness, opposite from the endothelial function augmentation observed after traditional hookah smoking, which is likely mediated by the large CO boost emitted from burning charcoal briquettes used to heat the flavored tobacco; and 2) the processes of oxidative stress and inflammation play a pivotal mechanistic role underlying these vascular changes. In a cross-over study comparing traditional hookah smoking to e-hookah bowl inhalation, we will assess endothelial function measured by brachial artery flow- mediated dilation and aortic stiffness by pulse wave velocity and augmentation index in 18 young healthy hookah smokers 21-39 years old, before and after ad lib 30-minute smoking/ inhalation session. To test for oxidative stress mediation, we will determine if any acute impairment in endothelial function after e-hookah can be prevented by intravenous Vitamin C infusion, a potent anti-oxidant. Antioxidant defensive buffers, such as protective HDL or paraoxonase-1 activity, or an increase in oxidized lipoproteins will be collected. To test for inflammatory mediation, biomarkers including anti-inflammatory cytokine IL-10 and pro-inflammatory cytokines hsCRP, TNF-α, IL-6, IL-8 will be collected before and after the sessions as well as smoking exposure biomarkers (plasma nicotine and expired CO). The results of this proposal stand to fill in gaps in our mechanistic understanding of the comparative effect of traditional vs. e-hookah bowl on vascular and endothelial function and in so doing, inform policy decisions by the FDA about regulation of hookah products.

抽象的 水烟（水管）吸烟在全球范围内迅速增加。为这个流行做出贡献是 未经证实的人认为，传统的水烟烟雾通过水管时会被排毒。更多的 最近，电子水烟碗 - 具有电子加热但通过传统掺入的电子液体 水管 - 在美国认可的年轻女性成年人中，美国的受欢迎程度越来越高 营销声称这些产品比传统水烟更安全。与传统的水烟一起 除尼古丁和焦油外，吸烟者会暴露于富含碳的细颗粒和大量 碳一氧化碳（CO），后者已被证明构成了内皮依赖性血管扩张剂。 尽管毒物的暴露量远低于传统水烟，而没有任何CO暴露，但E-Hookah 碗提供构成推定的血管毒素的尼古丁和细颗粒。主要目标 项目是为了调查传统水烟吸烟与电子水烟碗的比较效果 对内皮和血管功能的吸入及其在心血管发展中的机械作用 疾病。特别是，我们将研究氧化应激和感染的作用 水烟引起的心血管疾病的作用。我们将检验以下假设。 1）在没有 木炭煤球和几乎任何CO暴露，e-Hookah碗吸入急性会急剧损害内皮 功能并唤起急性中心刚度，与观察到的内皮功能增强相反 传统的水烟吸烟，这很可能是由燃烧木炭发出的大型CO提升介导的 用于加热调味的烟草的煤球； 2）氧化应激和炎症的过程 这些血管变化的关键机理作用。在比较传统水烟的跨界研究中 吸烟到e-hookah碗吸入，我们将评估通过臂动脉流量测量的内皮功能 - 脉搏波速度和增强指数18 YOUN HEALTY 21-39岁的水烟吸烟者在30分钟的吸烟/吸入会议之前和之后。测试 氧化应激介导，我们将确定e-Hookah后内皮功能中是否有任何急性损害 可以通过静脉注射维生素C输注来预防，这是一种潜在的抗氧化剂。抗氧化剂防御缓冲液，例如 将收集保护性HDL或副氧酶-1活性，或收集氧化脂蛋白的增加。测试 炎症介导，包括抗炎细胞因子IL-10和促炎细胞因子在内的生物标志物 HSCRP，TNF-α，IL-6，IL-8将在会话之前和之后收集 生物标志物（血浆尼古丁和过期的CO）。该提案的结果将填补我们的空白 对传统与e-Hookah碗对血管和血管的比较效果的机械理解 内皮功能以及这样做，可以为FDA提供有关水烟产品调节的政策决策。