Discovery of Yellow Fever Virus-Specific Epitopes for Development of an Accurate Serodiagnostic Assay
Discovery of Yellow Fever Virus-Specific Epitopes for Development of an Accurate Serodiagnostic Assay
批准号:
9467246
负责人:
DAVID CAMERINI
金额:
$29.96万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-01-16 至 2019-12-31
关键词:
AedesAlgorithmsAlphavirusAntibodiesAntigensArbovirusesBiological AssayBrazilBurkholderia pseudomalleiChikungunya virusClinicalColombiaCross ReactionsCulicidaeDengue InfectionDengue VirusDetectionDevelopmentDiagnosticDiagnostic testsDiseaseDisease OutbreaksEpidemicEpitopesFlavivirusFutureGenomeGeographic stateGoalsHawaiiIndividualInfectionMelioidosisMethodsMexicoPathogenicityPatientsPatternPhaseProtein FragmentProtein MicrochipsProteinsProteomeProteomicsPublic HealthPuerto RicoRecording of previous eventsSalivaSamplingSerumSouth AfricaSouth AmericaSpecificityTechnologyTestingUniversitiesValidationViral ProteinsVirusWashingtonWest Nile viral infectionWest Nile virusYellow FeverYellow Fever Virus InfectionYellow fever virusZika Viruscross reactivityinnovationmortalitynovelnovel strategiespoint of careresponsesample collectionspecific biomarkerstool
中文摘要
黄热病病毒特异性抗原表位的发现及其在黄热病病毒诊断中的应用
血清诊断试验
摘要
在血液、血清和/或唾液中可以检测到许多致病病毒的抗体。其中
是许多虫媒病毒,在西半球和世界其他地区共享流行区域,
世界这些病毒包括相关的黄病毒黄热病病毒(YFV)、登革热病毒(DENV)、寨卡病毒(Zika virus)和寨卡病毒(Zika virus)。
ZIKV(ZIKV)和西尼罗河病毒(WNV)以及甲病毒基孔肯雅病毒(CHIKV)。抗体
这些病毒通常具有交叉反应性,使得对特定病毒的诊断具有挑战性。当前
巴西爆发黄热病,与这种疾病有关的死亡率很高,
近年来在南美和非洲爆发的历史创造了对YFV特异性疫苗的迫切需求。
血清学诊断,以帮助控制黄热病的传播。此外,这种诊断将有助于
针对这些和其他相关虫媒病毒的临床和公共卫生努力。 我们提出了一种新
开发特异性和灵敏性检测抗YFV抗体方法的策略,
与其他虫媒病毒交叉反应。我们将采用创新的、全面的蛋白质组学方法,
通过独特的血清样本采集,可以发现和验证特定的生物标志物
能够将YFV与其他虫媒病毒区分开。 这将使我们能够制定一个具体的和
YFV感染的敏感试验。一种实用、敏感、特异的黄热病病毒检测方法
由于最近在巴西爆发黄热病,感染是一个高度优先的目标,
黄热病的爆发和可能蔓延到美国南部与墨西哥湾接壤的州,
波多黎各,伊蚊属。蚊子可能会迅速传播感染。
英文摘要
Discovery of Yellow Fever Virus-Specific Epitopes for Development of an Accurate
Serodiagnostic Assay
Abstract
Antibodies to many pathogenic viruses can be detected in blood, serum and/or saliva. Among these
are many arboviruses, that share endemic regions in the western hemisphere and in other parts of the
world. These include the related flaviviruses yellow fever virus (YFV), dengue virus (DENV), Zika virus
(ZIKV) and West Nile virus (WNV) as well as the alphavirus chikungunya virus (CHIKV). Antibodies to
these viruses are often cross-reactive, making diagnostics for a particular virus challenging. The current
outbreak of yellow fever in Brazil and the high mortality associated with this disease as well as the
recent history of outbreaks in South America and Africa create an urgent need for YFV-specific
serodiagnostics, to aid control of the spread of yellow fever. Moreover, such diagnostics would facilitate
clinical and public health efforts against these and other, related arboviruses. We propose a novel
strategy to develop methods for specific and sensitive detection of antibodies against YFV that do not
cross react with other arboviruses. We will employ an innovative, comprehensive proteomic approach
with a unique serum sample collection that will allow discovery and validation of specific biomarkers
capable of distinguishing YFV from other arboviruses. This will allow us to develop a specific and
sensitive test for YFV infection in Phase II of this project. A practical, sensitive and specific test for YFV
infection is a high priority goal due to the recent outbreak of yellow fever in Brazil, possible future
outbreaks and the likely spread of yellow fever to southern US states bordering the Gulf of Mexico and
Puerto Rico where Aedes spp. mosquitos might rapidly spread the infection.
期刊论文(0)
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会议论文
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