Point of Care Detection of Oral Pathogens

口腔病原体的即时检测

• 批准号: 8973923
• 负责人: DAVID CAMERINI
• 金额: $ 22.5万
• 依托单位: IMMPORT THERAPEUTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-20 至 2017-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8973923
• 关键词: Acquired Immunodeficiency Syndrome; Antibodies; Antigens; Bacteria; Binding; Biological Assay; Candida albicans; Detection; Development; Devices; Disease; Ensure; Fusobacterium; Genes; HIV; HIV-1; Herpesvirus 1; Human Papillomavirus; Infection; Lactobacillus; Marketing; Microfluidic Microchips; Morbidity - disease rate; Oral cavity; Organism; Phase; Porphyromonas gingivalis; Prevotella intermedia; Procedures; Production; Protein Microchips; Proteins; Proteomics; Protocols documentation; Protozoa; Route; Saliva; Serotyping; Simplexvirus; Streptococcus; Streptococcus mutans; Viral Antigens; Viral Proteins; Virus; Virus Diseases; base; cost effective; experience; expression vector; influenzavirus; malignant oropharynx neoplasm; mortality; oral lesion; oral pathogen; pathogen; point of care; prototype; public health relevance; screening; transmission process

 DESCRIPTION (provided by applicant): There are many pathogenic organisms that cause disease in the oral cavity or that can be detected there. Among these are three important viruses, human immunodeficiency virus (HIV), human papilloma virus (HPV) and herpes simplex virus (HSV). HIV infection and disease is treatable but is costly and is still associated with considerable morbidity and mortality. HPV is the primary cause of oropharyngeal cancer and HSV causes common oral lesions. All of these pathogens can be spread by contact with saliva. This is not a common route of transmission for HIV, but it is nevertheless a concern since HIV causes AIDS which is a fatal disease. It would therefore be desirable to have a simple, cost-effective point-of-care (POC) device for the detection of infection by HIV, HPV and HSV. We propose to use a protein microarray to detect HIV by antigen capture and to detect antibodies to each virus based on binding to viral proteins in the microarray. Antigen Discovery Incorporated (ADi) has considerable experience in the development, production and marketing of protein microarrays. We now sell seventeen reactive protein antigen microarrays from a variety of pathogenic viruses, bacteria and protozoa including an HIV-1 array with proteins from the five most common subtypes. Moreover, ADi has already isolated all the genes from eleven serotypes of HPV, HSV types 1 and 2 and inserted them into an expression vector for production of proteins. In order to develop a useful POC device for the detection of HIV as well as antibodies against HIV, HPV and HSV in saliva, we have the following specific aims for the first phase of this project: 1. Create a proteomic microarray containing all proteins from all common types of HIV-1, HPV and HSV. Later we will expand the microarray to include other oral pathogens 2. Develop a protocol for sensitive and specific screening for antibodies to oral pathogens in saliva using a proteomic microarray. In Phase II a microfluidic device will be developed to automate the assay procedure. 3. Develop a sensitive and specific microarray-based antigen-capture detection assay for HIV. In Phase II similar assays will be developed for HPV, HSV and other oral pathogens and the assay procedure will be automated using a microfluidic device.

 描述（由申请人提供）：有许多致病微生物会导致口腔疾病或在口腔中检测到。其中有三种重要的病毒，人类免疫缺陷病毒（HIV）、人类乳头瘤病毒（HPV）和单纯疱疹病毒（HSV）。艾滋病毒感染和疾病是可以治疗的，但费用昂贵，而且发病率和死亡率仍然很高。HPV是口咽癌的主要原因，HSV引起常见的口腔病变。所有这些病原体都可以通过接触唾液传播。这不是艾滋病毒的常见传播途径，但它仍然令人关切，因为艾滋病毒导致艾滋病，这是一种致命的疾病。因此，期望具有用于检测HIV、HPV和HSV感染的简单、成本有效的护理点（POC）装置。我们建议使用蛋白质微阵列检测艾滋病毒的抗原捕获和检测抗体的基础上结合到病毒蛋白的微阵列中的每一种病毒。Antigen Discovery Incorporated（ADI）在蛋白质微阵列的开发、生产和销售方面拥有丰富的经验。我们现在出售17种来自各种致病病毒、细菌和原生动物的反应蛋白抗原微阵列，包括一种含有五种最常见亚型蛋白质的HIV-1微阵列。此外，ADi已经从HPV、HSV 1型和2型的11种血清型中分离出所有基因，并将它们插入表达载体中用于生产蛋白质。为了开发一种用于检测唾液中的HIV以及针对HIV、HPV和HSV的抗体的有用的POC装置，本项目的第一阶段的具体目标如下：1.创建包含所有常见类型HIV-1、HPV和HSV的所有蛋白质的蛋白质组微阵列。稍后，我们将扩大微阵列，包括其他口腔病原体2。开发一种使用蛋白质组学微阵列对唾液中口腔病原体抗体进行敏感和特异性筛选的方案。在第二阶段，将开发一种微流体装置来自动化检测程序。3.开发一种灵敏、特异的基于微阵列的HIV抗原捕获检测方法。在第二阶段，将开发用于HPV、HSV和其他口腔病原体的类似检测方法，并且检测程序将使用微流体装置自动化。