权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Perm1 in skeletal muscle dysfunction induced by disuse and heart failure

Perm1 在废用和心力衰竭引起的骨骼肌功能障碍中的作用

基本信息

批准号：
9889551
负责人：
Robert Scott Ross
金额：
--
依托单位：
VA SAN DIEGO HEALTHCARE SYSTEM
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-01 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9889551
关键词：
Acquired Immunodeficiency Syndrome Address Affect Bed rest Bioenergetics Biogenesis Ca(2+)-Calmodulin Dependent Protein Kinase Chronic Chronic Disease Congestive Heart Failure Data Denervation Dependovirus Diabetes Mellitus Disease ERR1 protein Exercise Functional disorder Future Gene Expression Gene-Modified Genes Hand Health Heart Heart Diseases Heart failure Immobilization Injury Knock-out Lead Malignant Neoplasms Mediating Metabolic Mitochondria Modeling Molecular Morphology Mus Muscle Muscle Fibers Muscle Mitochondria Muscle Proteins Muscle function Muscular Atrophy Organism PPAR gamma Pathologic Pathology Patients Phosphotransferases Physical Exercise Physical activity Population Protein Kinase Publishing Recovery Role Signal Pathway Signal Transduction Skeletal Muscle Striated Muscles Testing Transcription Coactivator Veterans Virus Work base calmodulin-dependent protein kinase II clinical application constriction estrogen-related receptor exercise capacity exercise intolerance functional adaptation gene therapy improved in vivo man mouse model muscle form muscle metabolism novel novel strategies prevent programs skeletal muscle wasting skeletal muscle weakness therapeutic evaluation transcription factor

项目摘要

Project Summary/Abstract Skeletal muscle (SkM) atrophy is a significant health problem associated with muscle disuse. It can frequently occur in association with diseases or treatments of any type that require bed rest or full immobilization. It can result nervous supply injury, or result from chronic diseases, such as cancer, AIDS, diabetes, and importantly, a focus of this proposal, chronic heart failure (HF). Increased physical activity and exercise reduce muscle atrophy or even accelerate its recovery, by causing SkM to undergo metabolic, contractile and morphological adaptations. These adaptations rely on signaling pathways that lead to changes in gene expression and enzymatic activity. Therefore, understanding the molecular mechanisms underlying muscle activity-induced signaling may provide novel basic information about muscle function and adaptation and ultimately could provide new approaches for treating muscle dysfunction, including muscle dysfunction in heart failure. We note that the transcriptional coactivator PGC-1 has been shown to be induced and activated by exercise, and regulate muscle metabolic programs. By using a microarray screen with a myotube model transduced with PGC-1 (PPARgamma coactivator 1) and ERR (Estrogen-related receptor) transcription factors, we identified a novel PGC-1 / ERR target gene, termed Perm1 (PGC-1 and ERR-induced regulator, muscle 1). Our published and preliminary data show that striated muscle protein (Perm1) impacts muscle bioenergetics, is induced by exercise, regulates expression of metabolic genes, and modifies muscle mitochondrial (Mito) biogenesis. Perm1 is also down-regulated in SkM with disuse and the presence of heart failure. Based on this strong preliminary data, we propose the central hypothesis that Perm1 integrates activity-induced signals and enhances muscle mitochondrial biogenesis and function. A corollary to this is that Perm1 acts by modulating activity/contraction–induced kinase signaling (CaMKII, calcium/calmodulin dependent protein kinase II). Through activity-induced signal integration, Perm1 may prevent disuse and HF - induced SkM dysfunction. To address this hypothesis, we have generated two novel mouse models in which Perm1 is either specifically increased in skeletal muscle (Perm1SkMTransgenic (TG)) or deleted from skeletal muscle (Perm1SkM knockout (KO)). This hypothesis will be pursued by the following specific aims: Aim 1. Study the role of Perm1 in metabolic and functional alterations induced by muscle disuse. We will use Perm1SkMTG and Perm1SkMKO mice to directly evaluate Perm1 function in basal and pathological states including denervation and cast immobilization. Aim 2. Study the role of Perm1 in Heart Failure-induced SkM dysfunction. We will study the role of Perm1 in HF-induced SkM dysfunction, comparing WT, to Perm1SkMTG, and Perm1SkMKO mice. We hypothesize Perm1SkMTG will be protected from HF-induced SkM weakness and exercise intolerance, while Perm1SkMKO mice will be susceptible to metabolic and functional deregulation induced by HF. Mechanism(s) by which Perm1 effects occur, such as CaMKII-related signaling will be pursued in this aim. Aim 3. Test the therapeutic benefits of Perm1 for treating SkM dysfunction induced by disuse and heart failure using a gene therapy approach. This aim will extend to translational work and test how alteration in Perm1 affects muscle function using an adeno-associated virus (AAV) -mediated gene therapy approach to test the therapeutic potential of Perm1 in ameliorating SkM dysfunction. We will test if augmentation of Perm1 expression in SkM will prevent or accelerate recovery from SkM dysfunction. The viruses and models are all in hand. This proposal should uncover important basic and translational information relevant to improve the health of Veterans with skeletal muscle dysfunction present from disuse and importantly, as a result of heart failure.