权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

High Throughput Digital Droplet ELISA for Ultrasensitive Multiplexed Diagnostics

用于超灵敏多重诊断的高通量数字液滴 ELISA

基本信息

批准号：
9889673
负责人：
David Aaron Issadore
金额：
$ 21.33万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-04 至 2023-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9889673
关键词：
Address Antibodies Attention Benchmarking Benign Biological Assay Biological Markers Cancer Center Cancer Diagnostics Cancer Patient Cellular Phone Clinical Clinical Sensitivity Cloud Computing Code Collaborations Color Complex DNA Detection Development Devices Diagnosis Diagnostic Disease Disease Progression Drug Targeting Electronics Emulsions Enzyme-Linked Immunosorbent Assay Fluorescence Gold Grant Heterogeneity Human Hybrids Individual Industry Laboratories Lasers Lesion Light Liquid substance Malignant - descriptor Malignant Neoplasms Malignant neoplasm of pancreas Measurement Measures Methods Microfluidic Microchips Microspheres Modality Molecular Monitor Nature Neoplasm Metastasis Nucleic Acids Patients Physiological Plasma Population Proteins Publishing RNA Reaction Reproducibility Research Resolution Running Sampling Sensitivity and Specificity Source Technology Telecommunications base biomarker panel cancer biomarkers cancer diagnosis chemical reaction circulating biomarkers clinical biomarkers clinical diagnostics cohort cost cytokine detector digital disorder control drug efficacy experimental study falls follow-up handheld mobile device human subject improved innovation instrumentation microfluidic technology molecular marker multiplex detection outcome forecast personalized medicine point of care portability protein biomarkers single molecule small molecule tool tumor

项目摘要

Abstract: Digital assays — in which ultra-sensitive molecular measurements are made by performing millions of parallel experiments in picoliter droplets — have generated much recent enthusiasm due to their single molecule resolution of RNA, DNA, and proteins, and their robustness to reaction conditions. These assays have enormous untapped potential for point of care disease diagnostics, but are currently mainly confined to laboratory settings due to the cumbersome instrumentation necessary to generate, control, and measure tens of millions of independent droplets. To overcome this challenge, we propose a hybrid microelectronic / microfluidic chip to `unlock' droplet-based assays for clinical use. Our microdroplet megascale detector (µMD) can generate and detect the fluorescence of millions of droplets per second (1000× faster than existing digital approaches), while achieving a 1000x greater sensitivity than conventional ELISA, using only a conventional cell phone camera. The key innovation of our approach is borrowed from the telecommunications industry, wherein we modulate the excitation light with a pseudorandom sequence that enables individual droplets to be resolved that would otherwise overlap due to the limited frame rate of digital cameras. To demonstrate the power of our approach, we focus our attention on the diagnosis of pancreatic cancer.

文摘: