Skin Procurement and Engineering

核心B，皮肤采购和工程核心的两个主要目标是：1)促进基本和 从正常人皮肤中获取新鲜皮肤和原代皮肤细胞进行皮肤生物学的翻译研究 和患病的人类和老鼠；以及2)通过改造正常的皮肤来促进皮肤研究的创新 以及在体外和体内转基因的人类皮肤。新鲜的人类皮肤和原代皮肤细胞高度 宝贵的资源，有助于将皮肤研究扩展到更大的科学社区，请访问我们的 皮肤科以外的大量调查人员证明了这一点 以前使用了我们的核心服务，随后提交了新的摘要、出版物和授权 在皮肤生物学方面的应用。通过将这些高利用率的初级组织服务与高度创新的 组织工程咨询和服务，核心B的目标是在更广泛的范围内最大化我们的影响 科学界。我们的长远目标是：(1)让内部和外部的调查人员 皮肤科通过集中进行皮肤生物学研究，更有效率和效率 提供大量或专门的技术服务，并提供关键的研究基础设施和机构 审查董事会对其使用的批准；(2)培养创新的探索性项目和初级皮肤调查人员 生物学通过我们的核心服务，目标是促进新的科学合作，摘要， 手稿和赠款；(3)通过提供正常和患病的信息来促进翻译研究 非内科医生可能无法获得的人类皮肤样本，或者有大量的行政管理 使用障碍；以及(4)将我们的核心董事的研究专业知识整合到未来的核心服务中，以适应 满足我们成员的新需求和新机遇。为实现这些目标，我们将提供：(1) 临床和科学专业知识、行政支持和服务，以获取新鲜正常人(成人或新生儿) 用于生理学和病理生理学研究的患病人体皮肤；(2)科学专长、行政管理 支持、服务和培训从新鲜的原代角质形成细胞、黑素细胞和成纤维细胞培养 人和鼠皮肤样本；(3)科学专门知识、行政支助、服务和培训，以 建立来源于正常或病变人皮肤或三维器官型人皮肤的异种移植 使用慢载体、shRNA或CRISPR/Cas9进行基因工程的人类皮肤重建 技巧。

The two major goals of Core B, the Skin Procurement and Engineering Core, are 1) to facilitate basic and translational research in skin biology through the acquisition of fresh skin and primary skin cells from normal and diseased humans and mice; and 2) to foster innovation in skin research through the engineering of normal and genetically modified human skin in vitro and in vivo. Fresh human skin and primary skin cells are highly valuable resources that help to extend skin research throughout the larger scientific community at our institution, evidenced by the large number of investigators outside the Department of Dermatology who have previously used our Core services and subsequently submitted new abstracts, publications, and grant applications on skin biology. By combining these highly utilized primary tissue services with highly innovative tissue engineering consultation and services, Core B aims to maximize our impact within an even broader scientific community. Our long-term objectives are to: (1) allow investigators both within and outside the Department of Dermatology to conduct research on skin biology more efficiently and effectively by centralizing high-volume or specialized technical services and providing the critical research infrastructure and institutional review board approvals for their use; (2) foster innovative exploratory projects and junior investigators in skin biology through our core services, with the goal of promoting new scientific collaborations, abstracts, manuscripts, and grants; (3) promote translational research by providing access to normal and diseased human skin samples that might not otherwise be available to non-physicians or have substantial administrative barriers to their use; and (4) integrate our core directors' research expertise into future core services to adapt to the emerging needs and opportunities of our membership. To accomplish these goals we will provide: (1) clinical and scientific expertise, administrative support, and service to procure fresh normal (adult or neonatal) and diseased human skin for physiologic and pathophysiologic studies; (2) scientific expertise, administrative support, service, and training to derive primary keratinocyte, melanocyte, and fibroblast cultures from fresh human and mouse skin samples; and (3) scientific expertise, administrative support, service and training to establish human skin xenografts derived from normal or diseased human skin, or 3-dimensional organotypic human skin reconstructs genetically engineered using lentivector-, shRNA-, or CRISPR/Cas9-based techniques.