Pathobiology and biomechanics of vocal fold dehydration

声带脱水的病理生物学和生物力学

• 批准号: 9763561
• 负责人: Abigail Cox
• 金额: $ 31.5万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9763561
• 关键词: Acute; Address; Adverse effects; Affect; Age; Animal Model; Animals; Behavior; Belief; Biochemical; Biological; Biology; Biomechanics; Biomedical Engineering; Chronic; Clinical; Complex; Data; Dehydration; Disease; Environment; Epithelial; Epithelium; Equilibrium; Event; Experimental Designs; Experimental Models; Goals; Grant; Health; Histologic; Homeostasis; Human; Hydration status; Impairment; Injury; Intervention; Investigation; Knowledge; Laboratories; Lamina Propria; Larynx; Link; Liquid substance; Location; Magnetic Resonance Imaging; Measures; Metabolism; Modeling; Molecular; Molecular Structure; Muscle; National Institute on Deafness and Other Communication Disorders; Oryctolagus cuniculus; Outcome; Pathologic; Phonation; Physiological; Physiology; Play; Predisposing Factor; Prevalence; Process; Protons; Recommendation; Rehydrations; Research; Research Project Grants; Role; Route; Science; Speech-Language Pathology; Structure; Surface; Techniques; Testing; Therapeutic; TimeLine; Tissues; Veterinary Pathology; Voice; Water; adverse outcome; aged; density; human subject; in vivo; insight; novel; preservation; prevent; response; vocal cord

PROJECT SUMMARY Optimal body hydration is considered imperative to preserving vocal health by many experts in the field. To this end, minimizing dehydration and increasing hydration is often recommended to prevent voice problems and as treatment for ongoing problems. The presumption underlying this recommendation is that dehydration induces adverse pathological and biomechanical changes to the vocal folds, and that speakers will develop maladaptive, phonotraumatic behaviors to compensate. Due to the prevalence of phonotraumatic voice problems, initiating hydration treatments has become a common, unfounded clinical recommendation. The critical, missing links in this argument are the absence of data demonstrating adverse pathobiological and biomechanical effects of dehydration, and a realistic model to test these data endpoints. A realistic model should have intact homeostatic mechanisms that regulate hydration in vivo and be amenable to rigorous control of hydration state. A rabbit animal model that is dehydrated systematically meets these requirements. The first goal of this proposal is to quantify the adverse pathobiological and biomechanical sequelae of acute dehydration. Systemic and surface dehydration will be induced to physiologically-relevant levels that have been shown to induce voice changes in human speakers. The distinct effects of systemic and surface dehydration will be quantified in young and old rabbits in order to delineate the influence of route of dehydration, as well as age, on fluid homeostasis. The second goal of this proposal is to establish whether systemic and surface rehydration can reverse adverse vocal fold changes induced by dehydration in young and old rabbits. The third goal of this proposal is to establish differential effects of chronic systemic and chronic surface dehydration in young and old rabbits. Chronic dehydration might not only increase impairments that are observed in acute dehydration, but may also reveal mechanisms of degeneration not identified in the first two goals. Pathobiological and biomechanical endpoints will include proton density MRI as a non-invasive measure of vocal fold hydration state, and histological, cellular, molecular, and biomechanical assessment of the vocal folds following dehydration and rehydration. By combining cellular, molecular, structural, and functional techniques, this comprehensive proposal seeks to shed fundamental insight into the effects of altered hydration state on vocal fold fluid, epithelium, lamina propria and muscle tissue so that validated scientific recommendations can be made on the adverse effects of dehydration and the therapeutic benefits of hydration.

项目摘要 该领域的许多专家认为，最佳的身体水合作用对于保持声乐健康至关重要。本 最后，通常建议尽量减少脱水和增加水分，以防止声音问题， 治疗持续存在的问题。这一建议的基本假设是， 不利的病理和生物力学变化的声带，扬声器将发展 适应不良的声音创伤行为来补偿由于普遍存在的语音创伤的声音 由于这些问题，开始水化治疗已经成为一种常见的、毫无根据的临床建议。的 在这一论点中，关键的缺失环节是缺乏证明不良病理生物学和 脱水的生物力学效应，以及一个现实的模型来测试这些数据的终点。一个现实的模型 应该具有完整的体内平衡机制，调节体内水合作用， 控制水化状态。系统脱水的兔动物模型符合这些要求。 该建议的第一个目标是量化急性心肌梗死的不良病理生物学和生物力学后遗症， 脱水全身和表面脱水将被诱导至生理相关水平， 已经被证明可以诱导人类说话者的声音变化。全身和表面的不同影响 将在年轻和年老的兔子中定量脱水，以描述给药途径的影响。 脱水以及年龄对体液平衡的影响该提案的第二个目标是确定是否 全身和表面再水化可以逆转年轻人脱水引起的不利声带变化 老兔子该提案的第三个目标是确定慢性全身性和慢性 幼兔和老年兔的表面脱水。慢性脱水不仅会增加 在急性脱水中观察到，但也可能揭示第一次未发现的变性机制。 两个目标。病理生物学和生物力学终点将包括质子密度MRI作为非侵入性 测量声带水合状态，以及组织学、细胞、分子和生物力学评估。 声带在脱水和再水化后的变化。通过结合细胞、分子、结构和 功能技术，这一全面的建议，旨在摆脱基本的洞察力的影响， 改变声带液、上皮、固有层和肌肉组织的水合状态， 可以对脱水的不良影响和脱水的治疗益处提出科学建议。 水合作用