Pathophysiology Core

病理生理学核心

• 批准号: 9898137
• 负责人: Roland Krug
• 金额: $ 127.31万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-25 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9898137
• 关键词: Algorithms; Anatomy; Assessment tool; Biochemical; Biochemistry; Biological; Biomechanics; Bone Marrow; Chronic low back pain; Clinical; Clinical Research; Collaborations; Communities; Consultations; Custom; DNA; Data; Data Analyses; Development; Education; Educational workshop; Equipment; Fatty acid glycerol esters; Flow Cytometry; Functional disorder; Funding; Genome; Goals; Histologic; Histology; Human; Image; Image Analysis; Individual; Injury; Link; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Measures; Messenger RNA; Methods; Mission; Modeling; Molecular; Multimodal Imaging; Muscle; Musculoskeletal; Permeability; Phenotype; Physiologic pulse; Physiological; Physiology; Positron-Emission Tomography; Protocols documentation; RNA; Research; Research Design; Research Personnel; Sampling; Serum; Serum Markers; Services; Site; Standardization; Structure; Suggestion; Techniques; Testing; Texture; Tissue Sample; Tissue imaging; Tissues; Training; Translational Research; Validation; Visualization software; base; biopsychosocial; clinically relevant; image processing; imaging informatics; imaging modality; in vivo imaging; innovation; instrument; miRNA expression profiling; morphometry; novel; outreach; patient oriented; pre-clinical; preservation; psychologic; quantitative imaging; ranpirnase; reconstruction; single photon emission computed tomography; social; tool; web portal

The goal of the PathPhys-Core is to provide state-of-the-art, customized quantitative serum, tissue, and imaging methods to characterize tissue anatomy, damage, and injury in the context of an integrated, biopsychosocial model of cLBP. Additionally, the PathPhys-Core will promote the development and validation of new tools to help identify the molecular, cellular, biochemical, and structural underpinnings of cLBP. The PathPhys Core will consist of three Sub-Cores for Imaging, Tissue Histology, and Serum Analysis. These Sub- Cores will: 1) provide consultation regarding human and sample analyses; 2) standardize protocols; 3) assist with data interpretation; and 4) drive the development of new tools for analyzing pathophysiology and injury. In the context of the overall mission of BACPAC and to support the goals of REACH, the PathPhys-Core proposes the following Specific Aims centered around two themes: innovation and service; education and dissemination. Innovation and Service: Goal is to provide the unique tools for UCSF REACH to characterize tissue anatomy, damage, and injury in the context of cLBP using novel physiology assessment tools. Aim 1: To provide, maintain, and support state-of-the-art quantitative serum, tissue, and imaging methods for pre-clinical and human musculoskeletal tissues. Examples include: MRI pulse sequences for the assessment of endplate structure and permeability (UTE), bone marrow fat content and texture, muscle volume and muscle fat (IDEAL), and disc structure and composition (T2, T1rho). Aim 2: To develop and support novel quantitative serum, tissue, and imaging methods and grading tools. The core will leverage an existing Quantitative Image Processing Center (QUIPC) to provide training, templates as well as software for visualization, post-processing, and quantitative analysis. Examples include: Develop novel image analysis techniques such as texture-based BMF or T1rho distribution, voxel- based morphometry and relaxometry, muscle volume and endplate segmentation algorithms etc. Aim 3: To provide interpretation of imaging data and assess links to the extracted biochemical, histological, and biomechanical measures, and the relevant clinical endpoints. Education and Dissemination: Goal is to disseminate information from the PathPhys Core to the BACPAC consortium, increase research collaborations between sites, and provide educational sessions. Aim 4: To provide web portal access to examples of previous studies that have used serum/tissue/imaging endpoints and detailed descriptions of clinical and research services, and to handle new service requests efficiently through the web portal. Online requests from new users for research collaboration and service will be reviewed by the staff and consultation with appropriate individuals will be set up. Post-processing and imaging informatics will be provided to assist researchers in study design and application. A goal will be to make suggestions as to how best integrate quantitative imaging and analysis into the studies being proposed. Aim 5: To perform proactive outreach to attract potential new users and participate in the BACPAC consortium activities to provide imaging seminars, workshops, and facilities tours that will introduce new imaging and image processing capabilities to the BACPAC community.

PathPhys-Core的目标是提供最先进的定制定量血清、组织和 成像方法，以表征组织解剖结构，损伤，和损伤的背景下， cLBP的生物心理社会模型。此外，PathPhys-Core将促进开发和验证 新的工具，以帮助确定分子，细胞，生化和结构基础的cLBP。的 PathPhys Core将由成像、组织组织学和血清分析三个子核心组成。这些次- 核心将：1）提供有关人体和样本分析的咨询; 2）标准化方案; 3）协助 与数据解释;和4）推动新的工具，用于分析病理生理学和损伤的发展。 在BACPAC的总体使命背景下，为了支持REACH的目标，PathPhys-Core 提出了以下具体目标围绕两个主题：创新和服务;教育和 传播。 创新和服务：目标是为UCSF REACH提供独特的工具来表征组织解剖结构， 损伤和损伤的情况下，使用新的生理学评估工具的cLBP。 目标1：提供、维护和支持最先进的定量血清、组织和成像方法 用于临床前和人体肌肉骨骼组织。示例包括： 终板结构和渗透性（UTE）、骨髓脂肪含量和质地、肌肉 体积和肌肉脂肪（IDEAL），以及椎间盘结构和组成（T2，T1 rho）。 目的2：开发和支持新的定量血清，组织和成像方法和分级工具。 该核心将利用现有的定量图像处理中心（QUIPC）提供培训， 模板以及可视化、后处理和定量分析软件。示例 包括：开发新的图像分析技术，如基于纹理的BMF或T1 rho分布，体素- 基于形态测量和松弛测量，肌肉体积和终板分割算法等。 目的3：提供成像数据的解释，并评估与提取的生化，组织学， 和生物力学指标，以及相关的临床终点。 教育和传播：目标是从PathPhys核心向BACPAC传播信息 联盟，增加研究中心之间的合作，并提供教育会议。 目的4：提供门户网站访问先前使用血清/组织/成像的研究示例 端点和临床和研究服务的详细描述，并处理新的服务请求 有效地通过门户网站。新用户对研究合作和服务的在线请求 将由工作人员进行审查，并与适当的个人进行协商。后处理 和影像信息学将提供帮助研究人员在研究设计和应用。一个目标将 如何最好地将定量成像和分析整合到正在进行的研究中， 提出了 目标5：积极主动地开展外联活动，吸引潜在的新用户并参与BACPAC 联盟活动，提供成像研讨会，讲习班和设施图尔斯，将介绍新的 图像和图像处理能力的BACPAC社区。