Pathophysiology Core

病理生理学核心

• 批准号: 10765799
• 负责人: Roland Krug
• 金额: $ 58.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-25 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10765799
• 关键词: Pathophysiology; Core

The goal of the PathPhys-Core is to provide state-of-the-art, customized quantitative serum, tissue, and imaging methods to characterize tissue anatomy, damage, and injury in the context of an integrated, biopsychosocial model of cLBP. Additionally, the PathPhys-Core will promote the development and validation of new tools to help identify the molecular, cellular, biochemical, and structural underpinnings of cLBP. The PathPhys Core will consist of three Sub-Cores for Imaging, Tissue Histology, and Serum Analysis. These Sub- Cores will: 1) provide consultation regarding human and sample analyses; 2) standardize protocols; 3) assist with data interpretation; and 4) drive the development of new tools for analyzing pathophysiology and injury. In the context of the overall mission of BACPAC and to support the goals of REACH, the PathPhys-Core proposes the following Specific Aims centered around two themes: innovation and service; education and dissemination. Innovation and Service: Goal is to provide the unique tools for UCSF REACH to characterize tissue anatomy, damage, and injury in the context of cLBP using novel physiology assessment tools. Aim 1: To provide, maintain, and support state-of-the-art quantitative serum, tissue, and imaging methods for pre-clinical and human musculoskeletal tissues. Examples include: MRI pulse sequences for the assessment of endplate structure and permeability (UTE), bone marrow fat content and texture, muscle volume and muscle fat (IDEAL), and disc structure and composition (T2, T1rho). Aim 2: To develop and support novel quantitative serum, tissue, and imaging methods and grading tools. The core will leverage an existing Quantitative Image Processing Center (QUIPC) to provide training, templates as well as software for visualization, post-processing, and quantitative analysis. Examples include: Develop novel image analysis techniques such as texture-based BMF or T1rho distribution, voxel- based morphometry and relaxometry, muscle volume and endplate segmentation algorithms etc. Aim 3: To provide interpretation of imaging data and assess links to the extracted biochemical, histological, and biomechanical measures, and the relevant clinical endpoints. Education and Dissemination: Goal is to disseminate information from the PathPhys Core to the BACPAC consortium, increase research collaborations between sites, and provide educational sessions. Aim 4: To provide web portal access to examples of previous studies that have used serum/tissue/imaging endpoints and detailed descriptions of clinical and research services, and to handle new service requests efficiently through the web portal. Online requests from new users for research collaboration and service will be reviewed by the staff and consultation with appropriate individuals will be set up. Post-processing and imaging informatics will be provided to assist researchers in study design and application. A goal will be to make suggestions as to how best integrate quantitative imaging and analysis into the studies being proposed. Aim 5: To perform proactive outreach to attract potential new users and participate in the BACPAC consortium activities to provide imaging seminars, workshops, and facilities tours that will introduce new imaging and image processing capabilities to the BACPAC community.

PathPhys-Core的目标是提供最先进的、定制的定量血清、组织和 成像方法来描述组织解剖、损伤和损伤的特征， 慢性下腰痛的生物心理社会模型。此外，PathPhys-Core将促进开发和验证 帮助确定cLBP的分子、细胞、生化和结构基础的新工具。这个 PathPhys核心将由成像、组织组织学和血清分析三个子核心组成。这些附属公司- 核心将：1)提供有关人类和样本分析的咨询；2)标准化方案；3)协助 数据解释；以及4)推动用于分析病理生理学和损伤的新工具的开发。 在BACPAC的总体使命和支持REACH目标的背景下，PathPhys-Core 围绕两个主题提出以下具体目标：创新和服务；教育和 传播。 创新和服务：目标是为加州大学旧金山分校REACH提供独特的工具来表征组织解剖学， 在cLBP的背景下，使用新的生理学评估工具进行损伤和损伤。 目标1：提供、维护和支持最先进的定量血清、组织和成像方法 用于临床前和人体肌肉骨骼组织。示例包括：磁共振脉冲序列 终板结构和通透性(UTE)、骨髓脂肪含量和质地、肌肉的评估 体积和肌肉脂肪(理想)，以及椎间盘结构和成分(T2，T1rho)。 目的2：开发和支持新的定量血清、组织和成像方法和分级工具。 该核心将利用现有的定量图像处理中心(QUIPC)来提供培训， 模板以及用于可视化、后处理和定量分析的软件。示例 包括：开发新的图像分析技术，如基于纹理的BMF或T1Rho分布，体素- 基于形态测量和松弛测量、肌肉体积和终板分割算法等。 目标3：提供成像数据的解释，并评估与提取的生化、组织学、 和生物力学指标，以及相关的临床终点。 教育和传播：目标是将信息从PathPhys核心传播到bacpac 联盟，增加网站之间的研究合作，并提供教育会议。 目标4：提供使用血清/组织/成像的先前研究的实例的Web门户访问 终端和临床和研究服务的详细描述，并处理新的服务请求 通过门户网站高效地提供服务。新用户对研究协作和服务的在线请求 将由工作人员进行审查，并将与适当的个人进行磋商。后处理 影像信息学将帮助研究人员进行研究设计和应用。一个目标将会 就如何最好地将定量成像和分析整合到研究中提出建议 建议。 目标5：开展积极主动的推广活动，吸引潜在的新用户并参与bacpac 提供成像研讨会、研讨会和设施参观的联盟活动，这些活动将介绍新的 向bacpac社区提供成像和图像处理能力。