Low Cost Point-Of-Care Test For CMV

项目摘要 先天性巨细胞病毒(CMV)感染是导致出生缺陷和发育障碍的主要原因 在美国，每年影响数以千计的儿童。当前的方法(例如，组织培养，贝壳小瓶检测， 单拷贝基因聚合酶链式反应等。检测CMV感染是劳动密集型的，需要复杂的技术 设备、专用实验室空间和训练有素的技术人员。一种用于新生儿筛查的快速现场测试 可以在临床标本中检测到CMV的方法将：(1)允许立即咨询和额外的标本 收集用于验证性测试，以及(2)避免不得不为广泛传播的 生物菌素试验。我们建议开发一种快速筛查先天性巨细胞病毒的护理点检测方法。 感染。与医院和临床相比，这项技术还将显著降低成本 病理学实验室测试。该项目的目标是开发一种廉价(10-15美元每个样品)和快速 (从标本到结果的30分钟)原发CMV感染的筛查试验。

Project Summary Congenital Cytomegalovirus (CMV) infection is a leading cause of birth defects and developmental disabilities in the U.S., affecting thousands of children each year. Current methods (e.g., tissue culture, shell vial assay, single-copy gene PCR methods, etc.) to detect CMV infections are labor intensive and require sophisticated equipment, dedicated laboratory space, and trained technicians. A rapid, on-site test for newborn screening that could detect CMV in clinical specimens would: (1) allow for immediate counseling and additional specimen collection for confirmatory testing, and (2) avoid having to create new laboratory infrastructure for widespread biospecimen testing. We propose developing a point-of-care test for rapid screening of congenital CMV infection. This technology will also result in a significant cost reduction as compared to hospital and clinical pathology laboratory tests. The goal of this project is to develop an inexpensive ($10-15 per sample) and rapid (<30 min from specimen to result) screening assay for primary CMV infections.