Core 1: Microfabication

项目摘要/摘要：核心1(缩微技术) 微制造核心是一个虚拟基础设施，建立在 范博士和列夫琴科博士的实验室，一直致力于创新的微流体和 用于癌细胞生物分子和表型分析的生物微机械系统平台。它也得到了 耶鲁大学西校区的共享设施。具体地说，核心将通过以下方式支持这两个研究项目 制造和供应纳米图形阵列基板和微芯片平台以及 与它们一起执行的化验服务以i)测量单个癌细胞的迁移和侵袭性 结合外部线索和基因改变来确定导致P-A的分子网络 表型开关，以及ii)测量一组旁分泌信号，介导异型细胞-细胞通讯 驱动非细胞自主的侵袭性传播机制。同时，核心将是一项技术 创新中心将开发新的技术平台，为科学发现提供新的机会 这是使用现有工具无法实现的。因此，核心将建立在大量研究的基础上 工程创新是耶鲁大学癌症系统生物学中心的重要组成部分。 核心项目和研究项目的整合将根据以下研究计划进行。 第一，用于定向细胞迁移测量的纳米图形图案化阵列基板的制备。 第二，通过整合上述底物，研制出微流控细胞侵袭力检测芯片 通过微流控来控制细胞培养液。该平台将用于分析黑色素瘤和胶质母细胞瘤 细胞运动和量化表型异质性(项目1)，并测量基质细胞和 由其分泌的旁分泌因子(项目2)通过进一步与双重整合而对癌细胞侵袭性的影响 通道微流控共培养装置。第三，开发一种微流控细胞-细胞通讯分析方法 微芯片。这个平台将被用来分析细胞之间的通讯如何改变癌细胞的命运 非自主时尚(项目2)。核心1号还将作为连接工程学和生物学的基地 培训跨学科的癌症研究人员和外展活动。核心将致力于跨网络 通过提供微制造支持，与其他U54中心的资源核心进行协作和补充。

PROJECT SUMMARY/ABSTRACT: Core 1 (Microfabication) The Microfabrication Core is a virtual infrastructure built upon the established microfabrication capacities in the laboratories of Drs. Fan and Levchenko, which have been geared toward innovative microfluidics and bioMEMS platforms for biomolecular and phenotypic analyses of cancer cells. It is also supported by the shared facility at Yale University West Campus. Specifically, the Core will support both Research Projects by fabricating and supplying nano-topographic patterned array substrata and microchip platforms as well as the assay services performed with them to i) measure single cancer cell migration and invasiveness in response to combination of external cues and genetic alterations to identify the molecular networks responsible for the P-A phenotypic switch, and ii) measure a panel of paracrine signals mediating heterotypic cell-cell communication that drive non-cell-autonomous mechanisms of invasive spread. In parallel, the Core will be a technology innovation center will develop novel technology platforms to enable new opportunities for scientific discoveries that cannot be achieved using the existing tools. Thus, the Core will be built upon substantial research in engineering innovation and represent an important component of the Yale's Cancer Systems Biology Center. Integration of the Core and the Research Projects will be performed according to the following research plan. First, fabrication of the nano-tographic patterned array substrata for directed cell migration measurement. Second, developing a microfluidic cell invasiveness assay chip via integrating the aforementioned substrata with microfluidic control of the cell media. This platform will be used to analyze melanoma and glioblastoma cell motility and quantify phenotypic heterogeneity (Project 1), and measure the influence of stromal cells and paracrine factors secreted therefrom (Project 2) on cancer cell invasiveness via further integration with the dual channel microfluidic co-culture device. Third, developing a microfluidic cell-cell communication assay microchip. This platform will be used to analyze how cell-cell communication alters the fate of cancer cells in a non-autonomous fashion (Project 2). The Core 1 will also function as a base to bridge engineering and biology to train cross-disciplinary cancer researchers and outreach activities. The Core will commit in trans-network collaboration and complement the resources cores in other U54 centers by offer microfabrication support.