FDA FERN Microbiology and Chemistry Cooperative Agreement Programs: Enhanced Food Safety

共同主要研究者：Eric Petty和Hugh Maguire，博士 项目概要/摘要 CCAP：增强食品安全和食品防御能力 利用现有的化学设备平台、方法和人员，科罗拉多 公共卫生和环境部实验室将扩大食品防御和食品安全 能力和专业知识，以推进综合食品安全体系。作为对Fern National的赞美 计划办公室（NPO）需要紧急增援能力或其他任务，日常食品 将进行监视。每周对各种食物基质的抽样将轮流进行，以涵盖肉类， 水果和蔬菜、婴儿食品、饮料和即食食品。肉类和鳄梨目前 作为CDC/FDA NARMS计划和FDA高容量 监测启动项目也将纳入约40个国家的例行分析， 每月样品新的和独特的食物基质遇到可能作为基质延伸 项目通过每周持续使用所有各种平台和组织方法， 应急准备和分析能力将得到优化。增加LC工作人员的专业知识- MS/MS，增加接受过样品提取培训的工作人员人数， 专业知识是重点领域。向我们新成立的实验室质量保证部门提供的资金将 帮助我们获得ISO/IEC 17025认证。直接和频繁的沟通， FERN（NPO）将通过参加电话会议、出席国家FERN 会议、最新情况电子报告和数据传输。对整个FERN的承诺 通过积极参与分配的项目来证明项目的改进， 能力验证、方法验证和实验室内计划的研究活动。 增强食品安全和安全测试计划 作为在多个奖励年度向MCAP提供资金的直接结果， 科罗拉多实验室建立了坚实的基础，以提高微生物和化学 试验.关键人员将继续参与分析鳄梨的项目任务 样本中是否存在沙门氏菌和单核细胞增生李斯特菌。工作人员将继续接受这一点 从国内来源的产品，以支持该计划。对该计划的早期评估 展示了高水平的成功。目前的计划包括继续执行这项任务， 增加指定进口产品的等效检测。两个新项目将在 第一年的延续奖，以加强现有的能力。一项多中心验证研究， 将在现有资金下完成的项目将得到实施，以支持目前的任务， 提供快速和灵敏的检测能力。建立新的检测方法将是 与现有的标准方法相结合，并应用于程序定义的其他目标 指令。目前正在考虑的这些矩阵扩展的例子是黄瓜和热 辣椒使用这些指定的方案将提供分析结果的一致性， 通过能力测试和现场作业的积极结果得到证明 到目前为止，该计划。科罗拉多实验室工作人员将参与所有提议或分配的活动 国家计划办公室支持加强食品安全。通过这一持续支持 由于科罗拉多实验室作为一个 扩大国家食品安全方案。

Co-Principal Investigators: Eric Petty and Hugh Maguire, PhD Project Summary / Abstract CCAP: Enhanced Food Safety and Food Defense Capacity and Capability Utilizing existing chemistry equipment platforms, methods and personnel the Colorado Department of Public Health and Environment Laboratory will expand food defense and food safety capacity and expertise to advance an integrated food safety system. As a compliment to FERN National Program Office (NPO) required emergency surge capacity or other assignments, routine food surveillance will be conducted. Weekly sampling of a variety of food matrices will rotate to cover meats, fruits and vegetables, baby foods, beverages and ready to eat foods. Meats and avocados currently collected for microbiological analyses as part of the CDC/FDA NARMS program and the FDA High Volume Surveillance Activation Project will also be incorporated into the routine analyses of approximately 40 samples per month. New and unique food matrices encountered may serve as matrix extension projects. Through continual weekly usage of all the various platforms and methods organizational emergency preparedness and analyst competence will be optimized. Increasing staff expertise in LC- MS/MS, expanding the number of staff trained in sample extraction and the duplication of instrumental expertise are areas of focus. Funds provided to our newly created laboratory Quality Assurance Unit will aid in our efforts toward ISO/IEC 17025 accreditation. Direct and frequent communication with the FERN (NPO) will be maintained through participation in conference calls, attendance at National FERN meetings, electronic reporting of status updates and data transmission. A commitment to overall FERN Program improvement will be demonstrated through active involvement in assigned projects, proficiency tests, method validations and intra-laboratory planned research activities. MCAP: Enhanced Food Safety and Security Testing Program As a direct result of the funding provided throughout multiple award years to the MCAP at the Colorado laboratory solid foundations were established for enhanced microbiological and chemical testing. Key personnel will continue participation in the program assignment of analyzing avocado samples for the presence of Salmonella and Listeria monocytogenes. Staff will continue to accept this product from domestic sources in support of the program. An early assessment of this program demonstrates a high level of success. Current plans include the continuation of this assignment and will add equivalent testing of imported product as assigned. Two new projects will be undertaken during the first year of the continuation award to enhance existing capabilities. A multicenter validation study that will be completed under current funding will be implemented to support current assignments and provide rapid and sensitive detection capabilities. Establishment of new detection methods will be combined with existing standard methods and applied to additional targets as defined by program directives. Examples currently under consideration for these matrix extensions are cucumbers and hot peppers. Use of these designated protocols will provide consistency of analytical findings as demonstrated through the positive outcomes of both proficiency tests and live assignments supported by the program to date. Colorado laboratory staff will engage in all activities proposed or assigned by the national program office in support of enhanced food safety. Ongoing support through this continuation award will provide greater return on investment as the Colorado laboratory operates as an extension of the national program for food safety.