Mechanisms underlying orientation selectivity in the mature mouse retina
成熟小鼠视网膜方向选择性的潜在机制
基本信息
- 批准号:9894639
- 负责人:
- 金额:$ 3.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2020-09-30
- 项目状态:已结题
- 来源:
- 关键词:Action PotentialsAddressAlgorithmsAnimal BehaviorAnimalsAxonBehaviorBiological ModelsBrainBrain regionCalciumCellsDataDetectionDevelopmentDiseaseDorsalElectrophysiology (science)EnvironmentEyeFire - disastersGABA-A ReceptorGoalsGrantHealthImageInjectionsKnowledgeLabelLateral Geniculate BodyLearningLinkMeasuresMediatingMolecularMorphologyMusNeuronsNeurosciencesOrganismPathway interactionsPatternPharmacologyPopulationProcessPropertyReportingRetinaRetinal Ganglion CellsSensoryShapesSignal TransductionStimulusStructureSynapsesTestingTimeTracerTrainingTransgenic OrganismsViralVisualVisual PathwaysVisual system structureWild Type MouseWorkbasecell typeconditional mutantexperiencefeature extractionganglion cellgenetic manipulationin vivomolecular markerneuromechanismorientation selectivitypatch clampreceptive fieldreceptorrelating to nervous systemresponseretinal axonretinal imagingvisual informationvisual processing
项目摘要
Project Summary
Orientation selectivity, a fundamental feature observed throughout the early visual system, is first
computed in the retina by orientation selective ganglion cells (OSGCs). Recent work has reported
diverse mechanisms underlying On and Off OSGCs, yet less is known about the On-Off type. My
preliminary calcium imaging data suggests that On-Off OSGCs are a major OSGC subtype in the
mouse retina and they are sensitive to GABAA receptor antagonists. This proposal aims to
determine the synaptic mechanisms underlying the orientation selectivity of On-Off OSGCs in the
mouse retina and their central projection patterns. The lack of molecular markers for OSGCs has
limited a mechanistic understand of orientation selectivity in the mammalian retina. In Aim 1, we
will perform calcium imaging of RGCs to screen for On-Off OSGCs and target them for single-cell
recordings. Additionally, we will use pharmacology and α-subunit specific manipulations of GABAA
receptors on RGCs to determine the involvement of specific synapses in orientation selectivity. In
Aim 2, we will identify the projection patterns of On-Off OSGCs to dorsal LGN using viral
retrograde tracers and transgenic labeling. Learning to perform whole-cell patch-clamp recordings
and retrograde tracer injections are major training goals of this grant. Results from this work will
yield a mechanistic understanding of retinal orientation selectivity and provide a link between this
retinal feature selectivity and higher visual processing in the thalamocortical pathway.
项目概要
方向选择性是整个早期视觉系统中观察到的一个基本特征,它是第一个
由定向选择性神经节细胞(OSGC)在视网膜中计算。近期工作已报道
On 和 Off OSGC 的机制多种多样,但人们对 On-Off 类型知之甚少。我的
初步钙成像数据表明,开关 OSGC 是一种主要的 OSGC 亚型
小鼠视网膜对 GABAA 受体拮抗剂敏感。该提案旨在
确定开关 OSGC 方向选择性的突触机制
小鼠视网膜及其中心投影图案。 OSGCs 分子标记的缺乏
限制了对哺乳动物视网膜方向选择性的机械理解。在目标 1 中,我们
将对 RGC 进行钙成像,以筛选 On-Off OSGC 并将其作为单细胞目标
录音。此外,我们将使用 GABAA 的药理学和 α 亚基特异性操作
RGC 上的受体以确定特定突触参与方向选择性。在
目标 2,我们将使用病毒识别 On-Off OSGC 到背侧 LGN 的投射模式
逆行示踪剂和转基因标记。学习进行全细胞膜片钳记录
逆行示踪剂注射是本次资助的主要培训目标。这项工作的结果将
产生对视网膜方向选择性的机械理解并提供这之间的联系
丘脑皮质通路中的视网膜特征选择性和更高的视觉处理能力。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Hector Acaron其他文献
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{{ truncateString('Hector Acaron', 18)}}的其他基金
Dendritic integration at the retinogeniculate synapse
视网膜原突触处的树突整合
- 批准号:
10596474 - 财政年份:2022
- 资助金额:
$ 3.29万 - 项目类别:
Dendritic integration at the retinogeniculate synapse
视网膜原突触处的树突整合
- 批准号:
10389037 - 财政年份:2022
- 资助金额:
$ 3.29万 - 项目类别:
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