Mechanisms underlying orientation selectivity in the mature mouse retina

项目摘要 在整个早期视觉系统中观察到的一个基本特征--定向选择性是第一 在视网膜中由定向选择性神经节细胞(OSGCs)计算。最近的研究报告说 在OSGCS和OSGCS的基础上存在多种机制，但对OSGCS的开关类型了解较少。我的 初步的钙成像数据表明，OSGCs是OSGC的一个主要亚型 小鼠视网膜，对GABAA受体拮抗剂敏感。这项建议旨在 确定OSGCS在脑内定位选择性的突触机制 小鼠视网膜及其中央投射图案。缺乏OSGCS的分子标记 限制了对哺乳动物视网膜定向选择性的机械理解。在目标1中，我们 我将对视网膜神经节细胞进行钙成像，以筛选开-关OSGCs，并将其定位于单细胞 录音。此外，我们还将使用GABAA的药理学和α亚基特异性操作 视网膜节细胞上的受体，以确定特定突触参与定向选择性。在……里面 目的2.利用病毒技术鉴定OSGCs向背侧LGN的投射模式 逆行示踪和转基因标记。学习进行全细胞膜片钳记录 逆行注射示踪剂是这笔赠款的主要培训目标。这项工作的成果将 从机制上理解视网膜取向的选择性，并提供两者之间的联系 丘脑皮质通路中视网膜特征的选择性和更高的视觉处理能力。

Project Summary Orientation selectivity, a fundamental feature observed throughout the early visual system, is first computed in the retina by orientation selective ganglion cells (OSGCs). Recent work has reported diverse mechanisms underlying On and Off OSGCs, yet less is known about the On-Off type. My preliminary calcium imaging data suggests that On-Off OSGCs are a major OSGC subtype in the mouse retina and they are sensitive to GABAA receptor antagonists. This proposal aims to determine the synaptic mechanisms underlying the orientation selectivity of On-Off OSGCs in the mouse retina and their central projection patterns. The lack of molecular markers for OSGCs has limited a mechanistic understand of orientation selectivity in the mammalian retina. In Aim 1, we will perform calcium imaging of RGCs to screen for On-Off OSGCs and target them for single-cell recordings. Additionally, we will use pharmacology and α-subunit specific manipulations of GABAA receptors on RGCs to determine the involvement of specific synapses in orientation selectivity. In Aim 2, we will identify the projection patterns of On-Off OSGCs to dorsal LGN using viral retrograde tracers and transgenic labeling. Learning to perform whole-cell patch-clamp recordings and retrograde tracer injections are major training goals of this grant. Results from this work will yield a mechanistic understanding of retinal orientation selectivity and provide a link between this retinal feature selectivity and higher visual processing in the thalamocortical pathway.