Genetically Mediated Hair Cell Degeneration in 3D Inner Ear Organoids

项目总结 这份提案描述了一项为期5年的培训计划，以培养一名临床医生-科学家研究员。 干细胞生物学和大规模基因组学。此应用程序的主要目标是作为 培训Rick Nelson、医学博士和博士，掌握成为独立科学调查员所需的关键技能 研究内耳毛细胞变性。纳尔逊博士目前是印第安纳大学的助理教授 在医学院，他将通过课程学习、大学资源和 辅导。听力损失和前庭功能障碍影响着全球数百万人和普通 病理生理学表现为对机械敏感的毛细胞丢失。Hashino博士将担任Nelson博士的 主要的导师，并培训他的干细胞生物学。Hashino博士是干细胞生物学的国际领先者 培养了众多博士后研究员和临床科学家。理查德·J·H·史密斯，加州大学医学博士 爱荷华州将担任纳尔逊博士的共同导师，并对他进行大规模基因组方法和生物信息学方面的培训。 史密斯博士是享誉国际的临床医生、科学家和人类遗传学领域的领军人物。纳尔逊博士还将 注册印第安纳大学的独立调查员孵化器(I3)计划，该计划在以下方面提供指导 科学和职业发展。D.Wade Clapp医学博士是一位国际公认的临床医生和科学家 将担任纳尔逊博士的职业顾问。他的咨询委员会将提供广泛的专业知识 研究规划和职业发展。在这项提议中，PI将建立一种新的毛细胞模型 使用小鼠干细胞衍生的含有机械敏感毛细胞的3D培养物进行退化。CRISPR 基因编辑技术将被用来破坏Tmprss 3基因，该基因已知会导致毛细胞 老鼠和人类的退化。接下来，PI将使用蛋白质组学方法来建立蛋白质 毛细胞中Tmprss3丝氨酸蛋白酶的相互作用体。最后，单细胞rna测序(rna-seq) 这项技术将被用于研究导致毛细胞退化的基因表达的全球变化。这个 科学环境，印第安纳大学耳鼻喉科和 在国际知名导师的共同努力下，这个项目变得可行。这些因素最大限度地增加了 实现纳尔逊博士长期的职业目标--成为一名独立的临床医生-研究员并发现 预防或延缓毛细胞退化的新靶点。

PROJECT SUMMARY This proposal describes a 5-year training program for the development of a clinician-scientist investigator in stem cell biology and large-scale genomics. The primary goal of this application is to serve as a basis for training Rick Nelson, MD, PhD in the critical skills necessary to become an independent scientific investigator studying inner ear hair cell degeneration. Dr. Nelson is currently an Assistant Professor at Indiana University School of Medicine where he will expand his scientific skills through course work, university resources and mentoring. Hearing loss and vestibular dysfunction affect millions of people worldwide and the common pathophysiologic finding is loss of the mechanosensitive hair cells. Eri Hashino, PhD will serve as Dr. Nelson's primary mentor and train him in stem cell biology. Dr. Hashino is an international leader is stem cell biology and has trained numerous postdoctoral fellows and clinician-scientists. Richard JH Smith, MD at University of Iowa will serve as Dr. Nelson's co-mentor and train him in large-scale genomic approaches and bioinformatics. Dr. Smith is an internationally renowned clinician-scientist and leader in human genetics. Dr. Nelson will also enroll in the Independent Investigator Incubator (I3) program at Indiana University, which provides guidance in scientific and career development. D. Wade Clapp, MD is an internationally recognized clinician-scientist who will serve as a career advisor for Dr. Nelson. His advisory committee will provide broad expertise that spans research planning and career development. In this proposal, the PI will establish a novel model of hair cell degeneration using mouse stem cell-derived 3D cultures containing mechanosensitive hair cells. CRISPR gene editing technology will be used to disrupt the Tmprss3 gene, which is known to cause hair cell degeneration in mice and humans. Next, the PI will use proteomic approaches to establish the protein interactome of the Tmprss3 serine protease in hair cells. Finally, single-cell RNA sequencing (RNA-seq) technology will be used to study global alterations in gene expression that lead to hair cell degeneration. The scientific environment, support from the Department of Otolaryngology at Indiana University and the internationally renowned mentors together make this project feasible. These factors maximize the potential to attain Dr. Nelson's long-term career goal of becoming an independent clinician-investigator and discovering new targets to prevent or delay hair cell degeneration.