Genetically Mediated Hair Cell Degeneration in 3D Inner Ear Organoids

3D 内耳类器官中遗传介导的毛细胞变性

• 批准号: 10132291
• 负责人: Rick F Nelson
• 金额: $ 17.88万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10132291
• 关键词: 3-Dimensional; Advisory Committees; Affect; Antibodies; Binding Proteins; Bioinformatics; Biological Assay; Biological Process; C-terminal; CASP3 gene; CRISPR/Cas technology; Cell Count; Cell Death; Cell physiology; Cells; Cleaved cell; Clustered Regularly Interspaced Short Palindromic Repeats; Cochlea; DNA Sequence Alteration; Data; Development; Disease model; Doctor of Philosophy; Enrollment; Environment; Epithelial; Epitopes; Exhibits; Family; Functional disorder; Gene Expression; Gene Expression Profile; Genes; Genetic; Genomic approach; Genomics; Goals; Hair Cells; Hour; Human; Human Genetics; Immunoprecipitation; In Vitro; Incubators; Indiana; Integral Membrane Protein; International; Iowa; Knockout Mice; Knowledge; Label; Labyrinth; Lead; Maintenance; Mass Spectrum Analysis; Mediating; Mentors; Methods; Modeling; Mus; Mutation; Nature; Organoids; Otolaryngology; Pathway Analysis; Pathway interactions; Patients; Pattern; Postdoctoral Fellow; Potassium Channel; Prevention; Process; Program Development; Protease Domain; Proteins; Proteomics; Research; Research Personnel; Role; Scanning Electron Microscopy; Scientist; Sensory; Sensory Hair; Serine Protease; Structure; System; Technology; Testing; Therapeutic Intervention; Time; Training; Training Programs; Universities; University resources; Vestibular Hair Cells; Work; career; career development; deaf; embryonic stem cell; equilibration disorder; experimental study; extracellular; genome editing; hearing impairment; in vivo; medical schools; member; novel; ototoxicity; preservation; prevent; professor; programs; progressive hearing loss; single-cell RNA sequencing; skills; stem cell biology; stem cells; three dimensional cell culture; three-dimensional modeling; transcriptome; transcriptome sequencing

PROJECT SUMMARY This proposal describes a 5-year training program for the development of a clinician-scientist investigator in stem cell biology and large-scale genomics. The primary goal of this application is to serve as a basis for training Rick Nelson, MD, PhD in the critical skills necessary to become an independent scientific investigator studying inner ear hair cell degeneration. Dr. Nelson is currently an Assistant Professor at Indiana University School of Medicine where he will expand his scientific skills through course work, university resources and mentoring. Hearing loss and vestibular dysfunction affect millions of people worldwide and the common pathophysiologic finding is loss of the mechanosensitive hair cells. Eri Hashino, PhD will serve as Dr. Nelson's primary mentor and train him in stem cell biology. Dr. Hashino is an international leader is stem cell biology and has trained numerous postdoctoral fellows and clinician-scientists. Richard JH Smith, MD at University of Iowa will serve as Dr. Nelson's co-mentor and train him in large-scale genomic approaches and bioinformatics. Dr. Smith is an internationally renowned clinician-scientist and leader in human genetics. Dr. Nelson will also enroll in the Independent Investigator Incubator (I3) program at Indiana University, which provides guidance in scientific and career development. D. Wade Clapp, MD is an internationally recognized clinician-scientist who will serve as a career advisor for Dr. Nelson. His advisory committee will provide broad expertise that spans research planning and career development. In this proposal, the PI will establish a novel model of hair cell degeneration using mouse stem cell-derived 3D cultures containing mechanosensitive hair cells. CRISPR gene editing technology will be used to disrupt the Tmprss3 gene, which is known to cause hair cell degeneration in mice and humans. Next, the PI will use proteomic approaches to establish the protein interactome of the Tmprss3 serine protease in hair cells. Finally, single-cell RNA sequencing (RNA-seq) technology will be used to study global alterations in gene expression that lead to hair cell degeneration. The scientific environment, support from the Department of Otolaryngology at Indiana University and the internationally renowned mentors together make this project feasible. These factors maximize the potential to attain Dr. Nelson's long-term career goal of becoming an independent clinician-investigator and discovering new targets to prevent or delay hair cell degeneration.

项目总结

项目成果

Human cochlear diffusion from the cerebrospinal fluid space with gadolinium contrast.

与钆对比的人耳蜗从脑脊液空间的扩散。

• DOI: 10.1016/j.ymthe.2023.08.001
• 发表时间: 2023
• 期刊: Molecular therapy : the journal of the American Society of Gene Therapy
• 作者: Totten,DouglasJ;Booth,KevinTA;Mosier,KristineM;Cumpston,EvanC;Whitted,Cody;Okechuku,Vanessa;Koontz,NicholasA;Nelson,RickF
• 通讯作者: Nelson,RickF

Biallelic Loss-of-Function Variants in BICD1 Are Associated with Peripheral Neuropathy and Hearing Loss.

BICD1中的双重功能丧失变体与周围神经病和听力损失有关。

• DOI: 10.3390/ijms24108897
• 发表时间: 2023-05-17
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
• 影响因子: 5.6
• 作者: Hirsch, Yoel;Chung, Wendy K. K.;Novoselov, Sergey;Weimer, Louis H.;Rossor, Alexander;LeDuc, Charles A.;McPartland, Amanda J.;Cabrera, Ernesto;Ekstein, Josef;Scher, Sholem;Nelson, Rick F.;Schiavo, Giampietro;Henderson, Lindsay B.;Booth, Kevin T. A.
• 通讯作者: Booth, Kevin T. A.

TMPRSS3 expression is limited in spiral ganglion neurons: implication for successful cochlear implantation.

• DOI: 10.1136/jmg-2022-108654
• 发表时间: 2022-12
• 期刊: Journal of medical genetics
• 影响因子: 4