Non-Invasive, Quantitative, and Label-Free Characterization of Tissue Engineered Skeletal Muscle
Non-Invasive, Quantitative, and Label-Free Characterization of Tissue Engineered Skeletal Muscle
批准号:
9894756
负责人:
LISA M LARKIN
金额:
$20.59万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-04-01 至 2022-03-31
关键词:
3-DimensionalAddressAlgorithmsArchitectureCaringCell SurvivalClinicalClinical TrialsCollagenCollagen FiberDataData SetDiagnosticEngineeringEnvironmentEvaluationExposure toExtracellular MatrixFlavin-Adenine DinucleotideFluorescenceGenerationsGoalsGoldGrowthHealth Care CostsHistologicHourImaging TechniquesImplantIn VitroInjuryLGALS3BP geneLabelMeasurementMeasuresMechanicsMetabolicMetabolismMethodologyMethodsModalityModelingMolecular StructureMonitorMorbidity - disease rateMorphologyMuscleMuscle functionMyosin ATPaseNatural regenerationNicotinamide adenine dinucleotideOpticsOutcomePatientsProcessProductionProtocols documentationRattusResearchResearch PersonnelRotationSamplingSarcomeresSignal TransductionSiteSkeletal MuscleStandardizationSterilityStructureSurgical FlapsTechniquesTechnologyTestingTimeTissue EngineeringTissue ViabilityTissuesTranslatingTranslationsTraumabasebench to bedsidecombatcostdensitydisabilityexperienceextensor digitorumfluorophorefunctional outcomeshistological stainshistological studiesimaging approachimaging modalityimplantationimprovedin vivo regenerationlife time costmetabolic profilemolecular imagingmuscle engineeringnovelorganizational structurepreclinical developmentpreimplantationregenerativerepairedsample fixationsecond harmonicsecond harmonic generation imagingsuccesstissue regenerationtissue repairtoolusabilityvolumetric muscle losswound
中文摘要
当研究人员寻求将令人兴奋的技术转化为受损组织的再生时,缺乏非
英文摘要
As researchers seek to translate exciting technologies for the regeneration of damaged tissue, the lack of non-
invasive and sterile methods for quantitatively evaluating the quality of engineered tissues prior to use in
patients presents a significant challenge. Relatively few engineered tissue products have received FDA
approval, so many translational technologies have no antecedent as a guide for navigating the path from
bench to bedside. The proposed research intends to validate Optical Molecular Imaging (OMI) as a tool for
characterizing the viability of engineered skeletal muscle during and following fabrication, and predict
successful implantation that could also have broad applicability to other tissue engineering products. Current
evaluation of engineered skeletal muscle utilizes invasive histological staining or non-sterile functional testing.
Neither method avoids irrevocable damage of precious samples while simultaneously providing the quantitative
data required for rigorous product quality testing. To address this issue, we propose to use nonlinear OMI to
evaluate the metabolic activity and structural integrity of engineered skeletal muscle tissues during our tissue
fabrication process. Specifically, the fluorescence intensity of endogenous flavin adenine dinucleotide (FAD)
and nicotinamide adenine dinucleotide (NADH) will be detected and quantified through label-free OMI as a
measure of metabolic activity and cell viability. Additionally, the second harmonic generation signatures
generated by myosin in muscle sarcomeres and collagen fibers in the extracellular matrix will be quantified as
a measure of structural organization correlated with functional outcomes. By combining these two label free
imaging techniques, we will evaluate the metabolic and structural status of engineered skeletal muscle tissues
during fabrication. These characterized engineered tissues will then be implanted into a regenerative
environment to examine the ability of the proposed imaging method to predict successful repair of damage. It is
expected that the gold standards for evaluating skeletal muscle in vitro can be replicated non-invasively and
sterilely by this method. If successful for evaluating skeletal muscle, this technique could be applied to other
engineered tissues, allowing for reliable evaluation of a wide variety of tissue engineering technologies prior to
use as a repair modality.
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会议论文
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批准号:10724487
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资助金额:$12.1万
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依托单位:
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资助金额:$29.5万
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财政年份:2007
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Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
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批准号:7758217
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项目类别:
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资助金额:$30.73万
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财政年份:2007
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负责人:LISA M LARKIN
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依托单位:
Electromechanical Stimuli on the Development of Engineered Myotendinous Junction
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批准号:7177233
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项目类别:
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资助金额:$15.69万
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财政年份:2007
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负责人:LISA M LARKIN
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依托单位:
Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
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批准号:7574397
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项目类别:
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资助金额:$31.04万
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财政年份:2007
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负责人:LISA M LARKIN
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依托单位:
Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
-
批准号:7241958
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项目类别:
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资助金额:$32.8万
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财政年份:2007
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负责人:LISA M LARKIN
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依托单位:
Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
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批准号:7405408
-
项目类别:
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资助金额:$31.05万
-
财政年份:2007
-
负责人:LISA M LARKIN
-
依托单位:
Electromechanical Stimuli on the Development of Engineered Myotendinous Junction
-
批准号:7371936
-
项目类别:
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资助金额:$18.56万
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财政年份:2007
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负责人:LISA M LARKIN
-
依托单位:
Utilization of Engineered Skeletal Muscle Units to Repair Volumetric Muscle Loss
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批准号:8915801
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项目类别:
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资助金额:$15.55万
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财政年份:2007
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负责人:LISA M LARKIN
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依托单位:
Engineering Innervated Muscle-Tendon Constructs for Tissue Regeneration
-
批准号:8054547
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项目类别:
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资助金额:$2.14万
-
财政年份:2007
-
负责人:LISA M LARKIN
-
依托单位:
REHABILITATION OF MICRONEUROVASCULAR GRAFTS IN OLD RATS
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批准号:6042994
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项目类别:
-
资助金额:$10.7万
-
财政年份:1996
-
负责人:LISA M LARKIN
-
依托单位:
REHABILITATION OF MICRONEUROVASCULAR GRAFTS IN OLD RATS
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批准号:2001113
-
项目类别:
-
资助金额:$9.26万
-
财政年份:1996
-
负责人:LISA M LARKIN
-
依托单位:
REHABILITATION OF MICRONEUROVASCULAR GRAFTS IN OLD RATS
-
批准号:2457515
-
项目类别:
-
资助金额:$9.26万
-
财政年份:1996
-
负责人:LISA M LARKIN
-
依托单位:
REHABILITATION OF MICRONEUROVASCULAR GRAFTS IN OLD RATS
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批准号:2748476
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项目类别:
-
资助金额:$10.7万
-
财政年份:1996
-
负责人:LISA M LARKIN
-
依托单位:
REHABILITATION OF MICRONEUROVASCULAR GRAFTS IN OLD RATS
-
批准号:6168625
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项目类别:
-
资助金额:$10.71万
-
财政年份:1996
-
负责人:LISA M LARKIN
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依托单位:
海外基金