Monetite-Apatite Phase Transformation for an Enamel-Like Restorative Material

类牙釉质修复材料的三斜磷灰石-磷灰石相变

• 批准号: 9894790
• 负责人: Janet M. Oldak
• 金额: $ 24.75万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-15 至 2023-02-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9894790
• 关键词: Affect; Amalgam; Apatites; Biomechanics; Biomimetics; Ceramics; Chitosan; Crystallization; Dental; Dental Enamel; Dentin; Development; Exhibits; Future; Goals; Growth; Hardness; Human; Hydroxyapatites; In Situ; Ions; Kinetics; Longevity; Minerals; Modulus; Nanostructures; Outcome; Pathway interactions; Peptides; Phase; Polymers; Property; Research Proposals; Scheme; Structure; Surface; System; Testing; Time; Tissues; Tooth structure; alkalinity; amelogenin; base; calcium phosphate; cariogenic bacteria; chemical bond; chemical property; design; efficacy testing; experimental study; improved; inorganic phosphate; maleic acid; mechanical properties; mineralization; novel; physical property; preclinical efficacy; remineralization; restoration; restorative material; tooth surface

Project Summary / Abstract The goal of this exploratory/developmental research proposal is to explore a novel biomimetic calcium phosphate (CaP) composite material that will have an enamel-like structure and can be applied to restore posterior Class I cavities. Such an enamel-like biocomposite will integrate with the natural dental tissues through a strong chemical bond, and restore the tooth structures. It is based on the synthesis of a layered chitosan-monetite composite that can transform into an organized hydroxyapatite composite with improved mechanical properties. We demonstrated that that our newly designed amelogenin-inspired peptide P26 can promote regrowth of an enamel layer integrated with natural enamel and dentin. Building on these findings, we propose to apply the peptides to the monetite system in order to regulate the organized growth of apatite crystals at restoration-tooth interface. We hypothesize that i) organized enamel-like apatite crystals will be formed through a phase transformation of layered chitosan-monetite composite in the presence of amelogenin- inspired peptide ; and ii) the peptide will promote and control the in situ growth of organized apatite crystals at the enamel/dentin surface to integrate the biocomposite with tooth structures. We plan to test our hypotheses and approach the goal by pursuing the following three specific aims. Aim I) To synthesize a biomimetic chitosan-CaP composite with organized structure through a monetite-to-apatite phase transformation and to optimize its microstructure, biomechanical properties and setting time by incorporating an amelogenin-inspired peptide P26 and by examining the effects of additives such as Ca, phosphate, alkaline and amorphous calcium phosphate (ACP) on the abovementioned parameters. We hypothesize that incorporation of the small active peptides will assist in controlling the nanostructure of the final product and hence improve its hardness and elastic modulus. We will examine the release kinetics of mineralizing ions and P26 from the biocomposite material. Aim II) To evaluate integration of the biocomposite developed in Aim I to dental enamel and dentin using human extracted 3rd molar slabs. We will examine the interface between the biomimetic restorative material and the underlying tooth structures. We hypothesize that a strong chemical bond interface will be formed as the result of active peptide-guided remineralization at the tooth-restoration interface. If successful, this study will lead to an alternative dental restorative material to treat posterior Class I cavities, that has physical and chemical properties close to dental tissues (enamel/dentin), integrates well with the underlying tissues, and exhibits an extended longevity superior to the current restorative materials.

项目摘要/摘要 这一探索性/发展性研究提案的目标是探索一种新的仿生钙 磷酸盐(帽)复合材料，将具有釉质样结构，可用于修复 后方I类空洞。这种釉质状的生物复合材料将与天然的牙齿组织结合在一起 通过强烈的化学键，恢复牙齿结构。它是基于分层合成的 壳聚糖-芒硝酸盐复合材料可转化为有机羟基磷灰石复合材料的研究 机械性能。我们证明了我们新设计的釉原蛋白启发的多肽P26可以 促进釉质层再生，结合天然牙釉质和牙本质。基于这些发现，我们 建议将多肽应用于磷灰石体系，以调节磷灰石的有组织生长 修复体-牙界面处的晶体。我们假设i)有序的釉质状磷灰石晶体将是 在釉原蛋白存在下，通过层状壳聚糖-蒙脱石复合材料的相变而形成- 激发的多肽；以及ii)该多肽将促进和控制有序的磷灰石晶体的原位生长 牙釉质/牙本质表面，使生物复合材料与牙齿结构结合。我们计划检验我们的假设 并通过追求以下三个具体目标来实现这一目标。目的一)合成一种仿生剂 具有有序结构的壳聚糖帽复合材料，通过一氧化氮化物到磷灰石的相变和 通过加入釉原蛋白来优化其微观结构、生物力学性能和凝结时间 通过检测钙、磷、碱性和无定形钙等添加剂的作用 磷酸盐(ACP)对上述参数的影响。我们假设小活跃分子的公司 多肽将有助于控制最终产品的纳米结构，从而提高其硬度和 弹性系数。我们将研究矿化离子和P26从生物复合材料中的释放动力学 材料。目的二)评价在目标一中开发的生物复合材料与牙釉质和牙本质的结合 使用人类提取的第三磨牙板。我们将研究仿生修复剂之间的界面 材料和潜在的牙齿结构。我们假设强化学键界面会是 这是活性多肽引导的牙齿修复界面再矿化的结果。如果成功， 这项研究将导致一种替代的牙科修复材料来治疗后牙I类龋齿，它具有 接近牙齿组织(牙釉质/牙本质)的物理和化学特性，与底层结合良好 组织，并表现出延长寿命优于目前的修复材料。