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An Artificial Eggshell Membrane for Guided Tissue Regeneration

用于引导组织再生的人造蛋壳膜

基本信息

批准号：
9896507
负责人：
Yuanyuan Lucy Duan
金额：
$ 15.5万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-01 至 2022-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9896507
关键词：
Adult Animal Model Behavior Biocompatible Materials Biomimetics Birds Bone Regeneration Bone Tissue Cell Adhesion Cell Proliferation Cells Chronic Clinical Connective Tissue Craniofacial Abnormalities Data Defect Dental Dentistry Egg White Electrospinning Embryonic Development Environment Epithelial Epithelium Evaluation Fiber Food Industry Foundations Generations Gingiva Glycolates Goals Guided Tissue Regeneration Hydroxyapatites In Situ In Vitro Individual Industrial Product Inflammatory Ligaments Liquid substance Mechanics Medicine Membrane Membrane Proteins Methodology Molecular Morphology Outcome Oxygen Periodontal Diseases Periodontal Ligament Permeability Play Polymers Population Poultry Products Preparation Process Proteins Role Signal Transduction Structure Surface System Techniques Tissues Tooth Loss Waste Products Water base biomineralization bone bone loss cell behavior clinical predictors cost craniofacial bone crosslink improved in vivo evaluation innovation mechanical properties microorganism migration nano nanofiber novel orofacial particle prevent response tissue regeneration

项目摘要

Project Summary Periodontal disease is a chronic inflammatory process in the periodontal tissue. It occurs in approximately 48% of the U.S. adult population and is the leading cause of tooth loss among adults. Guided tissue generation (GTR) is a widely-used, well-documented and clinically predictable technique to treat periodontal disease and dental/craniofacial defects by placing a polymeric membrane between epithelial tissue and periodontal ligament/bone tissue. This membrane serves as a barrier to protect the slower-growing periodontal ligament and bone tissues from the invasion of faster-growing epithelial tissue, but it should also allow exchange of fluids and signals between different tissues. A number of barrier membranes have been developed over the past decades, but current products are very costly and have limitations. Avian eggshell membrane is a semi-permeable fibrous membrane between egg-white and eggshell. It is available in abundant quantities as a waste product from the poultry and food industry. Natural eggshell membrane is composed of a highly-crosslinked interwoven protein fiber network with a unique double-layered structure. It not only allows excellent water/oxygen exchange and satisfies the need of embryo development, but also serves as a natural barrier membrane against the invasion of microorganisms and other challenges from the external environment. Inspired by the similarity of eggshell membrane and GTR membrane as a barrier membrane, we propose this project to explore the feasibility of developing an innovative and biomimetic artificial eggshell membrane for GTR application using a reactive electrospinning technique and response surface methodology (RSM), which can simulate the unique composition and microstructure of natural eggshell membrane. Our new membrane is a multiple-component system which contains soluble eggshell membrane protein (SEP), mechanically stable poly (lactic-co-glycolic acid) (PLGA) and osseoinductive nano-hydroxyapatite particles (nanoHAp). It also possesses a bio- inspired double-layered structure, which includes an outer barrier PLGA layer (adjacent to the gingival tissue) and an inner bioactive SEP/PLGA/nano-HAp layer (adjacent to the periodontal/bone tissue). The long-term goal of our proposed project is to improve the clinical outcome of the GTR technique by using this biomimetic membrane, as well as lay a foundation for converting eggshell membrane derived materials from an abundant industrial by-product to a new, green and promising biomaterial for dentistry and medicine.

项目总结