Neural Networks of the Mouse Superior Colliculus

小鼠上丘的神经网络

• 批准号: 9768887
• 负责人: Nora Lissett Benavidez
• 金额: $ 4.5万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9768887
• 关键词: 3-Dimensional; Address; Anatomy; Animals; Anterior; Architecture; Area; Atlases; Attention; Attention deficit hyperactivity disorder; Axon; Behavior; Brain; Brain Stem; Brain region; Cell Nucleus; Communities; Computer software; Corpus striatum structure; Data; Data Analyses; Databases; Disease; Etiology; Eye Movements; Fiber; Foundations; Future; Genetic Engineering; Goals; Grant; Helper Viruses; Histology; Image; Imaging Techniques; Infection; Injections; Investigation; Label; Learning; Location; Logic; Mammals; Maps; Mediating; Mentorship; Methods; Microscopic; Microscopy; Midbrain structure; Morphology; Motor; Motor Cortex; Motor output; Mus; Neocortex; Neurodevelopmental Disorder; Neurons; Organizational Productivity; Output; Pathway interactions; Play; Population; Presynaptic Terminals; Process; Property; Rabies; Rabies virus; Resolution; Resources; Role; Saccades; Sensory; Solid; Structure; Synapses; Technical Expertise; Techniques; Technology; Tectum Mesencephali; Testing; Three-Dimensional Imaging; Tracer; Training; Viral; Work; association cortex; autism spectrum disorder; base; cell type; connectome; cortex mapping; directed attention; experimental study; lens; microscopic imaging; mouse model; multisensory; nervous system disorder; neural network; neuroinformatics; novel; online resource; reconstruction; relating to nervous system; sensory cortex; sensory input; superior colliculus Corpora quadrigemina; tool

PROJECT SUMMARY/ABSTRACT The midbrain superior colliculus (SC) receives extensive projections from sensory, motor and higher- association cortex, and projects downstream to brainstem motor nuclei. These cortico-tecto-brainstem projections are essential for attention-related motor eye movements. Despite this essential functional role and implications in attention-related disorders, little is known about the organization of cortico-tectal projections or their cell-type specific brainstem outputs. Neuroinformatics tools provide a computational capacity to analyze large-scale connectivity data in a reliable and feasible way to reveal novel connectivity organization networks. Previous studies reveal that cortical subnetworks topologically project to the striatum as functional domains. This suggests that cortico-tectal projections may also be organized into functional domains, and then descend to control downstream motor networks. Though classic tracers provide a wealth of input/output information of the SC, they cannot reveal synaptic specific connections. To reveal these connections and characterize their morphological properties, recently developed state-of-the-art rabies virus tracing methods and cutting-edge microscopy imaging provide new tools to elucidate specific pathways. The goal of this study is to construct a comprehensive wiring diagram of cortico-tecto-brainstem circuits that will be presented as an online resource to the scientific community. Specific Aim 1 will assemble a comprehensive connectivity projection map from the neocortex to the superior colliculus using classic anterograde/retrograde tracing techniques with neuroinformatics tools as part of the Mouse Connectome Project (www.MouseConnectome.org). This data will set an essential structural foundation to facilitate the study of specific attention-related circuits by revealing the organization of distinct functional subnetworks. Specific Aim 2 will define retrosplenial cortex (RSP) and anterior cingulate area (ACA) projections to distinct SC cell-types based on their anatomical locations, projection targets and neuronal morphologies. To define these cell types, this aim will use a monosynaptic rabies viral tracing method to map the synaptic connectivity between cortico-tectal inputs and brainstem- projecting SC neurons. The additional use of Lightsheet microscopy, SWITCH and 3D-reconstruction techniques utilized in pursuit of these aims render this project an ideal training venue. These studies will provide a more comprehensive foundation of cortical network organization within the SC that can also serve as a cross-species reference. Under the guidance of Dr. Hongwei Dong and colleagues in the Mouse Connectome Project, I will learn to perform high-quality histology, imaging and connectivity data analysis of SC neural networks. The mentorship and technical expertise I will acquire under this training grant will contribute greatly to my overall goal of becoming an independent neuroscientist studying detailed brain architecture.

项目摘要/摘要 中脑上丘（SC）获得了感官，运动和更高 - 结合皮层，并在脑干运动核下游项目。这些Cortico-Tecto-Brainstem 预测对于注意力相关的运动眼运动至关重要。尽管有一个基本的功能角色和 在与注意有关的疾病中的含义，对皮质直肠直肠预测的组织知之甚少 它们的细胞类型特定的脑干输出。神经信息学工具提供了分析的计算能力 以可靠且可行的方式揭示新的连接组织网络的大规模连接数据。 先前的研究表明，皮质子网在拓扑上将其作为功能域投射到纹状体上。 这表明皮层直肠投影也可以组织成功能域，然后下降 控制下游运动网络。尽管经典示踪剂提供了大量的输入/输出信息 SC，他们无法揭示突触特定的连接。揭示这些联系并描述他们的 形态学特性，最近发展的最先进的狂犬病病毒追踪方法和尖端 显微镜成像提供了阐明特定途径的新工具。这项研究的目的是构建 将作为在线资源呈现的Cortico-Tecto-Brainstem电路的全面接线图 向科学界。特定目标1将从 使用经典的逆行/逆行跟踪技术与上丘的新皮层 神经信息工具作为鼠标Connectome项目（www.mouseconnectome.org）的一部分。这些数据将 设定基本的结构基础，以促进特定注意相关电路的研究 组织独特的功能子网。特定的目标2将定义肾后皮质（RSP）和 基于解剖位置，对不同SC细胞类型的前扣带回区域（ACA）投影， 投影靶标和神经元形态。要定义这些细胞类型，此目标将使用单突触 狂犬病病毒追踪方法绘制皮质直肠输入与脑干之间的突触连通性 - 投影SC神经元。灯表显微镜，开关和3D重建的额外使用 用于追求这些目标的技术使该项目成为理想的培训场地。这些研究会 在SC中为皮质网络组织提供更全面的基础，也可以作为 跨物种参考。在Hongwei Dong博士和同事在鼠标的指导下 Connectome项目，我将学习执行SC的高质量组织学，成像和连接数据分析 神经网络。我将根据这笔培训赠款获得的指导和技术专长将贡献 我的总体目标是成为一个独立的神经科学家研究详细的大脑结构。