Multifunctional Ionic Liquids Coatings for Dental Implant Surfaces

用于牙种植体表面的多功能离子液体涂层

• 批准号: 9768425
• 负责人: Danieli Rodrigues
• 金额: $ 36.34万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-14 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9768425
• 关键词: Adhesions; Animal Model; Animals; Anions; Bacteria; Bacterial Adhesion; Bacterial Infections; Bone Growth; Bone Tissue; Cell surface; Cells; Clinical; Coculture Techniques; Corrosion; Corrosives; Dental Implants; Diagnostic radiologic examination; Drug Metabolic Detoxication; Elements; Environment; Epithelial; Etiology; Evaluation; Event; Excision; Experimental Designs; Failure; Female; Film; Friction; Generations; Gingiva; Gingival Crevicular Fluid; Goals; Growth; Histology; Immunohistochemistry; Impaired wound healing; Impairment; Implant; In Vitro; Incidence; Infection prevention; Inflammation; Inflammation Mediators; Inflammatory Response; Interruption; Kinetics; Liquid substance; Lubricants; Lubrication; Maxilla; Measurement; Measures; Methods; Microbial Biofilms; Modeling; Modification; Operative Surgical Procedures; Oral; Osseointegration; Outcome; Oxides; Performance; Perioperative; Phase; Postoperative Period; Process; Property; Race; Rattus; Reporting; Research; Research Personnel; Saliva; Series; Stress; Surface; Surface Properties; Techniques; Technology; Testing; Time; Tissues; Titanium; Torque; Toxic effect; Trauma; antimicrobial; base; biomaterial compatibility; biomineralization; bone; bone loss; cytotoxicity; design; experimental study; healing; implant design; implant material; implantable device; implantation; improved; in vivo; in vivo evaluation; liquid formulation; male; microbial; microbial colonization; microbial community; microbial host; microbiome; novel; oral condition; peri-implantitis; periodontopathogen; premature; preservation; prevent; recruit; response; seal; soft tissue; stem cell differentiation; success; surface coating; titanium dioxide

Multifunctional Ionic liquids Coatings for Dental Implant Surfaces Abstract Titanium dental implants are known for their high success rates and adequate osseointegration in vivo. However, with an increasing number of implants used, higher incidence of implant complications and failures have been recently reported. Implant failure is classified either as early or late stage failure. Early stage failure occurs when osseointegration is not achieved typically due to bacterial contamination, premature loading, excessive surgical trauma and impaired healing. Late stage failure occurs when implant osseointegration is lost having common etiological factors associated with bacterial-induced bone loss causing peri-implantitis, and excessive occlusal stresses. Recently, corrosion has also been considered a phenomenon underlying surface integration and performance. Bacteria seem to be key players in both early and late stage failures, with their presence influencing the establishment and loss of osseointegration. Hence, the success or failure of a dental implant can be related to its surface integration with soft and bone tissues versus biofilm adhesion. Early bacterial colonizers forming biofilms can impair soft tissue sealing by infiltrating and interrupting the process of surface integration. Current surface treatment techniques for dental implants typically aim to improve only one aspect of the problem, such as prevention of infection, promotion of osseointegration, or corrosion protection. However, to improve implant function, it is crucial to prevent early bacterial adhesion and to promote a permissive environment for tissue integration. In order to achieve multi-functionalities on implant surfaces, the goal of this proposal is to develop a new generation of coatings using ionic liquid (IL) technology. Non-toxic dicationic imidazolium-based IL coatings were designed to confer the surface of dental implants with: (i) antimicrobial activity for mitigation of biofilm adhesion, which will enable host cells to reach and seal the surface of the implant; (ii) protection of the oxide layer at the critical initial healing phase of the implant; and (iii) improved frictional properties for implant insertion. Aim 1 will study the competition of bacteria and host cells for the surface (“race for the surface”) of IL coated versus non-coated titanium using a co-culture approach. A co-culture model will be developed with conditions of varying concentrations of host and bacterial cells to test both peri-operative and post-operative models. In Aim 2, implants coated with the best-performing IL, as verified in Aim 1, will be investigated in an animal model. The effect of IL coating on the associated inflammatory response, soft and bone tissues, osseointegration, and bacterial load will be assessed at different time points simulating early and late healing periods. In vivo testing will enable observation of inflammatory responses and kinetics of bone growth triggered by the presence of IL- coated surfaces in comparison to non-coated implants. This new generation of coatings aims to mitigate bacteria adhesion on the surface following implantation while providing suitable surface conditions for tissue integration. Considering current surface treatments available, the proposed IL-coating may constitute a more potent strategy to improve dental implant surfaces.

用于牙科种植体表面的多功能离子液体涂层 摘要 钛牙科种植体以其高成功率和体内充分的骨整合而闻名。然而，在这方面， 随着使用的植入物数量的增加， 最近报道。植入物失效分为早期或晚期失效。早期故障发生在 通常由于细菌污染、过早加载、过度的外科手术 创伤和受损愈合。晚期失败发生在种植体骨整合丧失时， 与引起种植体周围炎和过度咬合的细菌性骨丢失相关的病因学因素 压力最近，腐蚀也被认为是表面整合的潜在现象， 性能细菌似乎是早期和晚期失败的关键参与者，它们的存在影响了 骨整合的建立和丧失。因此，种植牙的成功或失败可能与 与其表面与软组织和骨组织的整合相对于生物膜粘附。早期细菌定植者形成 生物膜可通过渗透和中断表面整合过程而损害软组织密封。电流 用于牙科植入物的表面处理技术通常旨在仅改善问题的一个方面， 例如预防感染、促进骨整合或防腐。然而，为了改善植入物 功能，它是至关重要的，以防止早期细菌粘附，并促进组织的环境许可 一体化为了实现植入物表面的多功能性，本提案的目标是开发一种 使用离子液体（IL）技术的新一代涂料。无毒的双阳离子咪唑鎓基IL涂料 旨在赋予牙种植体表面：（i）用于减轻生物膜的抗微生物活性 粘附，这将使宿主细胞能够到达并密封植入物的表面;（ii）保护氧化物 层在关键的初始愈合阶段的植入物;和（iii）改进的摩擦性能植入物插入。 目的1研究细菌和宿主细胞对白介素表面的竞争（“表面竞争”）， 与未涂覆的钛相比，使用共培养方法。将在以下条件下开发共培养模型： 不同浓度的宿主和细菌细胞以测试围手术期和手术后模型。在Aim中 2，将在动物模型中研究涂覆有性能最好的IL的植入物，如目标1中所验证的。的 IL涂层对相关炎症反应、软组织和骨组织、骨整合的影响， 在模拟早期和晚期愈合期的不同时间点评估细菌负荷。体内测试 将使得能够观察由IL-1的存在引发的炎症反应和骨生长的动力学。 涂层表面与非涂层植入物相比。这种新一代涂层旨在减少细菌 植入后粘附在表面上，同时为组织整合提供合适的表面条件。 考虑到目前可用的表面处理，所提出的IL涂层可能构成更有效的策略 以改善牙科植入物表面。