Multifunctional Ionic Liquids Coatings for Dental Implant Surfaces

用于牙种植体表面的多功能离子液体涂层

• 批准号: 10244903
• 负责人: Danieli Rodrigues
• 金额: $ 36.34万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-14 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10244903
• 关键词: Adhesions; Animal Model; Animals; Anions; Bacteria; Bacterial Adhesion; Bacterial Infections; Bone Growth; Bone Tissue; Cell surface; Cells; Clinical; Coculture Techniques; Corrosion; Corrosives; Dental Implants; Diagnostic radiologic examination; Drug Metabolic Detoxication; Elements; Environment; Epithelial; Etiology; Evaluation; Event; Excision; Experimental Designs; Failure; Female; Film; Friction; Generations; Gingiva; Gingival Crevicular Fluid; Goals; Growth; Histology; Immunohistochemistry; Impaired healing; Impairment; Implant; In Vitro; Incidence; Infection prevention; Inflammation; Inflammation Mediators; Inflammatory Response; Interruption; Kinetics; Liquid substance; Lubricants; Lubrication; Maxilla; Measurement; Measures; Methods; Microbial Biofilms; Modeling; Modification; Operative Surgical Procedures; Oral; Osseointegration; Outcome; Oxides; Performance; Perioperative; Phase; Postoperative Period; Process; Property; Race; Rattus; Reporting; Research; Research Personnel; Saliva; Series; Stress; Surface; Surface Properties; Techniques; Technology; Testing; Time; Tissues; Titanium; Torque; Toxic effect; Trauma; antimicrobial; base; biomaterial compatibility; biomineralization; bone; bone loss; cytotoxicity; design; experimental study; healing; implant design; implant material; implantable device; implantation; improved; in vivo; in vivo evaluation; liquid formulation; male; microbial; microbial colonization; microbial community; microbial host; microbiome; novel; oral condition; peri-implantitis; periodontopathogen; premature; preservation; prevent; recruit; response; seal; soft tissue; stem cell differentiation; success; surface coating; titanium dioxide

Multifunctional Ionic liquids Coatings for Dental Implant Surfaces Abstract Titanium dental implants are known for their high success rates and adequate osseointegration in vivo. However, with an increasing number of implants used, higher incidence of implant complications and failures have been recently reported. Implant failure is classified either as early or late stage failure. Early stage failure occurs when osseointegration is not achieved typically due to bacterial contamination, premature loading, excessive surgical trauma and impaired healing. Late stage failure occurs when implant osseointegration is lost having common etiological factors associated with bacterial-induced bone loss causing peri-implantitis, and excessive occlusal stresses. Recently, corrosion has also been considered a phenomenon underlying surface integration and performance. Bacteria seem to be key players in both early and late stage failures, with their presence influencing the establishment and loss of osseointegration. Hence, the success or failure of a dental implant can be related to its surface integration with soft and bone tissues versus biofilm adhesion. Early bacterial colonizers forming biofilms can impair soft tissue sealing by infiltrating and interrupting the process of surface integration. Current surface treatment techniques for dental implants typically aim to improve only one aspect of the problem, such as prevention of infection, promotion of osseointegration, or corrosion protection. However, to improve implant function, it is crucial to prevent early bacterial adhesion and to promote a permissive environment for tissue integration. In order to achieve multi-functionalities on implant surfaces, the goal of this proposal is to develop a new generation of coatings using ionic liquid (IL) technology. Non-toxic dicationic imidazolium-based IL coatings were designed to confer the surface of dental implants with: (i) antimicrobial activity for mitigation of biofilm adhesion, which will enable host cells to reach and seal the surface of the implant; (ii) protection of the oxide layer at the critical initial healing phase of the implant; and (iii) improved frictional properties for implant insertion. Aim 1 will study the competition of bacteria and host cells for the surface (“race for the surface”) of IL coated versus non-coated titanium using a co-culture approach. A co-culture model will be developed with conditions of varying concentrations of host and bacterial cells to test both peri-operative and post-operative models. In Aim 2, implants coated with the best-performing IL, as verified in Aim 1, will be investigated in an animal model. The effect of IL coating on the associated inflammatory response, soft and bone tissues, osseointegration, and bacterial load will be assessed at different time points simulating early and late healing periods. In vivo testing will enable observation of inflammatory responses and kinetics of bone growth triggered by the presence of IL- coated surfaces in comparison to non-coated implants. This new generation of coatings aims to mitigate bacteria adhesion on the surface following implantation while providing suitable surface conditions for tissue integration. Considering current surface treatments available, the proposed IL-coating may constitute a more potent strategy to improve dental implant surfaces.

牙科种植表面多功能离子液体涂料 摘要 钛种植体以其高成功率和体内充分的骨结合而闻名。然而， 随着种植体使用数量的增加，种植体并发症和失败的发生率也越来越高 最近报道的。种植失败分为早期失败和晚期失败。在以下情况下会出现早期故障 由于细菌污染、过早负荷、过度手术，通常不能实现骨整合。 创伤和愈合受阻。当种植体骨结合丢失时，会发生晚期失败 细菌引起的骨丢失导致种植体周围炎和咬合过度的病因 压力。最近，腐蚀也被认为是一种潜在的表面整合和 性能。细菌似乎是早期和晚期失败的关键因素，它们的存在影响着 骨整合的建立与丧失。因此，植牙的成败可能与植牙有关。 表面与软组织和骨组织结合，而不是生物膜粘连。早期细菌定殖体的形成 生物膜可以通过渗透和中断表面整合的过程来损害软组织的封闭。当前 用于牙科植入物的表面处理技术通常只旨在改善问题的一个方面，例如 作为预防感染，促进骨结合，或腐蚀保护。然而，要改进植入物 功能，防止早期细菌黏附和促进组织的宽松环境至关重要 整合。为了实现种植体表面的多功能，本方案的目标是开发一种 采用离子液体(IL)技术的新一代涂料。无毒双阳离子咪唑基离子液体涂料 旨在赋予牙科植入物表面：(I)减少生物膜的抗菌活性 粘附性，使宿主细胞能够到达并密封植入物表面；(Ii)氧化物的保护 在植入物的关键初始愈合阶段，(I)提高了植入物的摩擦力；(Ii)改善了植入物的摩擦性能。 目的1研究细菌和宿主细胞对白细胞介素膜表面的竞争(“表面竞赛”) 与使用共培养方法的非涂层钛相比。将开发一种共培养模式，条件是 不同浓度的宿主细胞和细菌细胞，以测试围手术期和术后模型。在AIM 2，将在动物模型中研究用目标1中验证的性能最好的IL涂层的植入物。这个 IL涂层对相关炎症反应、软组织和骨组织、骨整合和 细菌负荷将在模拟早期和晚期愈合期的不同时间点进行评估。体内试验 将能够观察由IL-2的存在引发的炎症反应和骨生长动力学。 涂层表面与非涂层植入物的比较。这一新一代涂层旨在减少细菌 植入后的表面黏附，同时为组织整合提供合适的表面条件。 考虑到目前可用的表面处理，拟议的IL-涂层可能构成更有效的策略 以改善牙种植体表面。