Bioprinting 3D skin for patient-specific drug discovery in inflammatory skin diseases.

生物打印 3D 皮肤,用于炎症性皮肤病患者特定药物的发现。

基本信息

项目摘要

Project Summary Psoriasis is an inflammatory skin disorder with abnormal epidermal hyperproliferation, which affects 2-3 % of the US population. There are no reliable in vitro models with which the efficacy and safety of psoriasis drug candidates can be tested. To meet this major unmet pharmaceutical need, we will combine iPSC reprograming and 3D bioprinting technologies to construct a 3D skin model to screen for drugs to treat this disease. iPSC methodology will be used, because iPSCs have unlimited proliferation potential and the capability to differentiate into different cell lineages, which allows us to circumvent the limitation imposed by the limited availability of primary cells that can be procured from patients. We successfully constructed 3D skin using exclusively iPSC-derived cells (fibroblasts and keratinocytes), demonstrating the feasibility of this approach. 3D bioprinting can allow us to reproducibly construct complex structures to recapitulate human tissues/organs. In this project, we will first construct immunocompetent 3D skin with primary keratinocytes, fibroblasts and T cells. Then, iPSCs will be reprogramed from fibroblasts or blood cells obtained from normal and psoriatic individuals. Keratinocytes and fibroblasts will be differentiated from iPSCs for construction of 3D skin models, whereas the abundant availability of T cells allows us to use cells directly from primary sources. Bioprinted psoriasis-specific iPSC derived skin constructs will be used for disease modeling and pharmacological validation. CRISPR technology will be used to integrate fluorescent reporters in iPSC-derived keratinocytes to monitor disease induction of psoriasis and effect of drug treatment. Completion of this project will provide us with a reliable 3D bioprinted model to develop drugs to treat psoriasis. Moreover, our 3D skin model can be easily adapted and modified to screen for drugs to treat other skin diseases, using a platform approach.
项目总结

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Angela M Christiano其他文献

A case of alopecia areata multiplex after injection of an autologous dermal micrograft for the treatment of androgenetic alopecia
注射自体真皮微移植治疗雄激素性脱发后并发多发性斑秃一例
  • DOI:
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Susumu Toshima;Yuichi Kurihara;Hisashi Nomura;Yuichiro Hayashi;Angela M Christiano;Masayuki Amagai;Noriko Umegaki-Arao
  • 通讯作者:
    Noriko Umegaki-Arao

Angela M Christiano的其他文献

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{{ truncateString('Angela M Christiano', 18)}}的其他基金

Influence of the Microbiome on the Natural History of Alopecia Areata
微生物组对斑秃自然史的影响
  • 批准号:
    10585677
  • 财政年份:
    2023
  • 资助金额:
    $ 63.88万
  • 项目类别:
Senescence-on-a-chip: Building a microphysiological 3D skin model
芯片衰老:构建微生理 3D 皮肤模型
  • 批准号:
    10685382
  • 财政年份:
    2022
  • 资助金额:
    $ 63.88万
  • 项目类别:
Senescence-on-a-chip: Building a microphysiological 3D skin model
芯片衰老:构建微生理 3D 皮肤模型
  • 批准号:
    10552430
  • 财政年份:
    2022
  • 资助金额:
    $ 63.88万
  • 项目类别:
A Multi-scale Atlas of Senescence in Diverse Tissue Types
不同组织类型衰老的多尺度图谱
  • 批准号:
    10385184
  • 财政年份:
    2021
  • 资助金额:
    $ 63.88万
  • 项目类别:
A Multi-scale Atlas of Senescence in Diverse Tissue Types
不同组织类型衰老的多尺度图谱
  • 批准号:
    10683316
  • 财政年份:
    2021
  • 资助金额:
    $ 63.88万
  • 项目类别:
Immunomonitoring and Microbiome Analysis in Alopecia Areata Patients Undergoing Fecal Microbiota Transplantation
接受粪便微生物群移植的斑秃患者的免疫监测和微生物组分析
  • 批准号:
    10157320
  • 财政年份:
    2021
  • 资助金额:
    $ 63.88万
  • 项目类别:
Immunomonitoring and Microbiome Analysis in Alopecia Areata Patients Undergoing Fecal Microbiota Transplantation
接受粪便微生物群移植的斑秃患者的免疫监测和微生物组分析
  • 批准号:
    10392950
  • 财政年份:
    2021
  • 资助金额:
    $ 63.88万
  • 项目类别:
Immunomonitoring and Microbiome Analysis in Alopecia Areata Patients Undergoing Fecal Microbiota Transplantation
接受粪便微生物群移植的斑秃患者的免疫监测和微生物组分析
  • 批准号:
    10414461
  • 财政年份:
    2021
  • 资助金额:
    $ 63.88万
  • 项目类别:
Clinical Trials in a Dish Using a Personalized Multi-Tissue Platform for Atopic Dermatitis
使用个性化多组织平台进行特应性皮炎的临床试验
  • 批准号:
    10038233
  • 财政年份:
    2020
  • 资助金额:
    $ 63.88万
  • 项目类别:
Drug Screening with a Biofrabricated 3-D Immunocompetent Skin Model for Drug Discovery in Psoriatic Disease
使用生物破碎 3D 免疫活性皮肤模型进行药物筛选,用于银屑病药物发现
  • 批准号:
    10249327
  • 财政年份:
    2020
  • 资助金额:
    $ 63.88万
  • 项目类别:

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