A Multi-scale Atlas of Senescence in Diverse Tissue Types
不同组织类型衰老的多尺度图谱
基本信息
- 批准号:10683316
- 负责人:
- 金额:$ 262.77万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-09-30 至 2026-08-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAddressAdultAffectAgeAgingAtlasesAutomobile DrivingAutopsyBiologicalBiology of AgingBloodBrainCell AgingCellsCentral Nervous SystemCerebrospinal FluidClinicalCollaborationsCommunitiesDataData AnalysesData CollectionData ScienceDermatologicDermatologyEnsureEnvironmentFosteringGene Expression ProfileGenomeGenomicsGeographic LocationsGoalsHeterogeneityHumanImageIndirect ImmunofluorescenceIndividualKnowledgeLeadershipLongevityMapsMedical centerModalityMolecularMolecular ProfilingNeurologyNew YorkPathologicPathologyPhasePhenotypePopulation HeterogeneityProceduresProcessProteomicsProtocols documentationReproductive BiologyResearchResearch PersonnelResolutionResourcesSamplingSkinSkin TissueSpinal CordStructureTechniquesTechnologyTissue BanksTissue ProcurementsTissue SampleTissuesUniversitiesVisualizationWorkage relatedbiobankcell typecomputerized toolsdata integrationethnic diversityexperienceexperimental studygenome-widegenomic datahuman tissueinnovationmolecular phenotypemulti-scale atlasmultidisciplinarymultiple omicsneuropathologynovelnovel markerpatient populationprospectiveprotein expressionracial diversityregenerativesenescencesingle nucleus RNA-sequencingsuccesssynergismtissue mappingtooltranscriptomics
项目摘要
OVERALL: PROJECT SUMMARY
Defining the molecular and cellular heterogeneity underlying senescent cell states is a critical knowledge gap in
the field. The Columbia University Senescence Tissue Mapping (CUSTMAP) Center is uniquely poised to
address this gap by creating a multi-scale atlas of senescence in diverse tissue types across the adult human
lifespan. CUSTMAP is a highly collaborative effort that builds upon long-standing and established collaborations
between Columbia University Irving Medical Center (CUIMC), The University of Edinburgh, New York University
(NYU), and the New York Genome Center (NYGC). Using state-of-the-art spatial genomics technologies and
leveraging our established experimental workflows and analytical pipelines, CUSTMAP will generate three-
dimensional maps of senescent cells in tissues with vulnerability to age-related degenerative processes: the
central nervous system (brain and spinal cord) and the skin. We will perform spatially resolved transcriptomics
(ST), single-nucleus RNA-sequencing, and multiplexed proteomics using iterative indirect immunofluorescence
imaging (4i) experiments in central nervous system (CNS) and skin tissues across the human lifespan, allowing
for unprecedented genome-wide molecular characterization at single-cell resolution in space. CUSTMAP is
structured around three scientific Cores, as well as an Administrative Core to support these integrated efforts.
Human tissue samples characterized and collected through the Biospecimen Core (BIO) will be analyzed using
the tools and techniques developed through the Biological Analysis Core (BAC) and Data Analysis Core (DAC),
leading to detailed maps of senescent cells and their effects in human tissues at single-cell resolution. The
success of CUSTMAP is predicated on access to a continuous supply of human tissues from healthy individuals
across the lifespan. CUSTMAP investigators have access to post-mortem samples and prospective tissue
collection from local resources at CUIMC as well as through established collaborations. Our unique geographic
location in Upper Manhattan enables tissue acquisition from a local population of patients that is rich in racial
and ethnic diversity. Thus, CUSTMAP is uniquely poised to obtain high-quality tissue from these diverse
populations and apply cutting-edge multiomic approaches to build integrated 3D molecular atlases of senescent
cells in CNS and skin tissues. Our approach provides a unique platform for driving transformative discoveries
of novel molecular, cellular and regional correlates of age-related changes in cellular senescence in human
tissues. The CUSTMAP workflow and computational tools are readily generalizable to other tissue types and
can be efficiently shared with and deployed across the SenNet Consortium.
总体:项目摘要
定义衰老细胞状态下的分子和细胞异质性是一个关键的知识缺口,
外地哥伦比亚大学衰老组织绘图(CUSTMAP)中心是独一无二的,
通过在成年人的不同组织类型中创建多尺度衰老图谱来解决这一差距
寿命CUSTMAP是一项高度协作的工作,建立在长期和既定的合作基础上
哥伦比亚大学欧文医学中心(CUIMC)、爱丁堡大学、纽约大学
(NYU)和纽约基因组中心(NYGC)。利用最先进的空间基因组学技术,
利用我们既定的实验工作流程和分析管道,CustMAP将产生三个-
易受年龄相关退行性过程影响的组织中衰老细胞的三维图谱:
中枢神经系统(脑和脊髓)和皮肤。我们将用空间分辨转录组学
(ST)单核RNA测序和使用迭代间接免疫荧光的多重蛋白质组学
在整个人类生命周期中对中枢神经系统(CNS)和皮肤组织进行成像(4 i)实验,
在太空中以单细胞分辨率进行前所未有的全基因组分子表征。CUSTMAP是
该中心围绕三个科学核心以及一个行政核心构建,以支持这些综合努力。
通过生物样本核心(BIO)表征和采集的人体组织样本将使用
通过生物分析核心(BAC)和数据分析核心(DAC)开发的工具和技术,
从而以单细胞分辨率绘制出衰老细胞及其在人体组织中的作用的详细图谱。的
CUSTMAP的成功取决于从健康个体获得持续的人体组织供应
在整个生命周期中。CUSTMAP研究人员可以获得死后样本和预期组织
从CUIMC的当地资源以及通过建立合作收集。我们独特的地理位置
位于上曼哈顿的位置使得能够从具有丰富种族背景的当地患者群体获得组织。
和种族多样性。因此,CUSTMAP是独一无二的,以获得高品质的组织,从这些不同的
人口和应用尖端的多组学方法来建立综合的3D分子图谱的衰老
CNS和皮肤组织中的细胞。我们的方法为推动变革性发现提供了独特的平台
新的分子,细胞和区域相关的年龄相关的变化在细胞衰老在人类
组织中CUSTMAP工作流程和计算工具易于推广到其他组织类型,
可以在SenNet Consortium之间高效共享和部署。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Angela M Christiano其他文献
A case of alopecia areata multiplex after injection of an autologous dermal micrograft for the treatment of androgenetic alopecia
注射自体真皮微移植治疗雄激素性脱发后并发多发性斑秃一例
- DOI:
- 发表时间:
2017 - 期刊:
- 影响因子:0
- 作者:
Susumu Toshima;Yuichi Kurihara;Hisashi Nomura;Yuichiro Hayashi;Angela M Christiano;Masayuki Amagai;Noriko Umegaki-Arao - 通讯作者:
Noriko Umegaki-Arao
Angela M Christiano的其他文献
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{{ truncateString('Angela M Christiano', 18)}}的其他基金
Influence of the Microbiome on the Natural History of Alopecia Areata
微生物组对斑秃自然史的影响
- 批准号:
10585677 - 财政年份:2023
- 资助金额:
$ 262.77万 - 项目类别:
Senescence-on-a-chip: Building a microphysiological 3D skin model
芯片衰老:构建微生理 3D 皮肤模型
- 批准号:
10685382 - 财政年份:2022
- 资助金额:
$ 262.77万 - 项目类别:
Senescence-on-a-chip: Building a microphysiological 3D skin model
芯片衰老:构建微生理 3D 皮肤模型
- 批准号:
10552430 - 财政年份:2022
- 资助金额:
$ 262.77万 - 项目类别:
A Multi-scale Atlas of Senescence in Diverse Tissue Types
不同组织类型衰老的多尺度图谱
- 批准号:
10385184 - 财政年份:2021
- 资助金额:
$ 262.77万 - 项目类别:
Immunomonitoring and Microbiome Analysis in Alopecia Areata Patients Undergoing Fecal Microbiota Transplantation
接受粪便微生物群移植的斑秃患者的免疫监测和微生物组分析
- 批准号:
10157320 - 财政年份:2021
- 资助金额:
$ 262.77万 - 项目类别:
Immunomonitoring and Microbiome Analysis in Alopecia Areata Patients Undergoing Fecal Microbiota Transplantation
接受粪便微生物群移植的斑秃患者的免疫监测和微生物组分析
- 批准号:
10392950 - 财政年份:2021
- 资助金额:
$ 262.77万 - 项目类别:
Immunomonitoring and Microbiome Analysis in Alopecia Areata Patients Undergoing Fecal Microbiota Transplantation
接受粪便微生物群移植的斑秃患者的免疫监测和微生物组分析
- 批准号:
10414461 - 财政年份:2021
- 资助金额:
$ 262.77万 - 项目类别:
Clinical Trials in a Dish Using a Personalized Multi-Tissue Platform for Atopic Dermatitis
使用个性化多组织平台进行特应性皮炎的临床试验
- 批准号:
10038233 - 财政年份:2020
- 资助金额:
$ 262.77万 - 项目类别:
Drug Screening with a Biofrabricated 3-D Immunocompetent Skin Model for Drug Discovery in Psoriatic Disease
使用生物破碎 3D 免疫活性皮肤模型进行药物筛选,用于银屑病药物发现
- 批准号:
10249327 - 财政年份:2020
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$ 262.77万 - 项目类别:
Bioprinting 3D skin for patient-specific drug discovery in inflammatory skin diseases.
生物打印 3D 皮肤,用于炎症性皮肤病患者特定药物的发现。
- 批准号:
9571254 - 财政年份:2017
- 资助金额:
$ 262.77万 - 项目类别:
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