Shared Resource: Proteomics and Metabolomics

共享资源：蛋白质组学和代谢组学

• 批准号: 9737413
• 负责人: Philip R Gafken
• 金额: $ 0.91万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9737413
• 关键词: Animal Organ; Biological Assay; Biological Models; Cancer Biology; Cancer Center Support Grant; Chromatography; Clinical; Consultations; Core Facility; Data Analyses; Development; Diagnostic tests; Doctor of Philosophy; Fred Hutchinson Cancer Research Center; Goals; High Pressure Liquid Chromatography; Informatics; Isotopes; Joints; Laboratories; Lipids; Malignant Neoplasms; Mass Spectrum Analysis; Medicine; Metabolic Pathway; Mission; Modification; Molecular; Molecular Weight; NMR Spectroscopy; Phospholipids; Post-Translational Protein Processing; Preparation; Proteins; Proteomics; Proteomics Shared Resource; Protocols documentation; Research; Research Personnel; Resource Sharing; Resources; Sampling; Serum; Services; Site; Testing; Time; Tracer; Training; Universities; Validation; Washington; anticancer research; aqueous; base; biomarker discovery; cost effective; experimental study; instrumentation; liquid chromatography mass spectrometry; member; metabolomics; metabolomics resource; operation; phosphoproteomics; protein complex; recruit; tool

Proteomics and Metabolomics Shared Resource Project Summary/Abstract The Proteomics and Metabolomics Shared Resource (PMSR) consists of a Fred Hutchinson Cancer Research Center (FHCRC)-based Proteomics/Metabolomics laboratory and a University of Washington-South Lake Union (UW-SLU) laboratory. The Proteomics section of the PMSR was formed in November 2002 with the goal of providing cost-effective high performance liquid chromatography and mass spectrometry-based proteomics services to Cancer Consortium (Consortium) members. The Proteomics resource's mission is to provide high quality service in a timely manner for small- and large-scale qualitative and quantitative proteomics analyses, protein modification characterization, and targeted proteomic analyses. These services have evolved from small-scale identification of protein complexes in model systems and identifying modifications on highly purified proteins to large-scale biomarker discovery/validation experiments in serum samples and phosphoproteomics analysis of animal organs. The PMSR received an outstanding merit assessment in 2008. In 2010, the PMSR was expanded to include metabolomics research and related resources through the joint recruitment of Daniel Raftery, Ph.D., a recognized leader in metabolomics research, to FHCRC and the University of Washington (UW). Along with the proteomics services mentioned above, the resource now provides untargeted profiling of aqueous metabolites and lipids (lipidomics), targeted profiling of metabolites from numerous metabolic pathways via mass spectrometry and nuclear magnetic resonance spectroscopy, and isotope tracer capabilities. The PMSR is also rapidly developing mass spectrometry phospholipid analysis. The resource is committed to developing and implementing new proteomics and metabolomics tools in order to uncover the molecular details that influence cancer biology and to support development of diagnostic and clinical tests. Scientific operations of the Proteomics and Metabolomics resource are divided between FHCRC and UW- medicine sites. Dr. Gafken oversees all operations at FHCRC and Dr. Raftery oversees those at UW. Drs. Gafken and Raftery meet on a monthly to coordinate activities, or more frequently if needed for specific projects. All proteomics-based projects are performed at FHCRC while metabolomics-based projects are divided between the two sites. Metabolomics projects for which established, routine assays are available are performed at FHCRC while those metabolomics projects that are non-routine and require significant development are performed at UW. As new metabolomics assays are developed and refined at the UW site, FHCRC-based staff will be trained on these protocols to make them more widely available as a service. All NMR capabilities and NMR-related projects will be conducted at UW-SLU.

蛋白质组学和代谢组学共享资源 项目摘要/摘要 蛋白质组学和代谢组学共享资源（PMSR）由弗雷德·哈钦森癌症研究组成 基于中心（FHCRC）的蛋白质组学/代谢组学实验室和华盛顿南部湖大学 工会（UW-SLU）实验室。 PMSR的蛋白质组学部分于2002年11月成立了目标 提供具有成本效益的高性能液相色谱和基于质谱的蛋白质组学 为癌症财团（财团）成员提供服务。蛋白质组学资源的使命是提供高 及时的高质量服务，用于小型和大规​​模定性和定量蛋白质组学分析， 蛋白质修饰表征和靶向蛋白质组学分析。这些服务从 对模型系统中蛋白质复合物的小规模鉴定，并确定对高度纯化的修改 在血清样品和磷蛋白质组学中的大规模生物标志物发现/验证实验的蛋白质 分析动物器官。 PMSR在2008年获得了出色的优点评估。 2010年，PMSR扩展到包括代谢组学研究和相关资源。 丹尼尔·拉夫特（Daniel Raftery，Ph.D. 华盛顿大学（UW）。以及上面提到的蛋白质组学服务，现在的资源 提供水性代谢物和脂质（脂质组学）的不靶向分析，代谢物的靶向分析 通过质谱和核磁共振光谱的众多代谢途径， 和同位素示踪功能。 PMSR也正在迅速发展质谱法磷脂 分析。该资源致力于开发和实施新的蛋白质组学和代谢组学工具 为了发现影响癌症生物学的分子细节并支持 诊断和临床测试。 蛋白质组学和代谢组学资源的科学操作分为FHCRC和UW- 药物网站。 Gafken博士监督FHCRC的所有操作，Raftery博士负责在UW负责。博士。 Gafken和Raftery每月开会以协调活动，或者在需要时更频繁地进行活动 项目。所有基于蛋白质组学的项目均在FHCRC上进行，而基于代谢组学的项目是 在两个站点之间分开。已建立，常规测定的代谢组学项目是 在FHCRC进行的，而那些非列表的代谢组学项目需要重要的 开发是在UW上进行的。随着新代谢组学测定的开发和完善， 基于FHCRC的员工将接受这些协议的培训，以使其更广泛地作为服务。全部 NMR功能和与NMR相关的项目将在UW-SLU进行。