Shared Resource: Proteomics & Metabolomics

项目摘要：蛋白质组学与代谢组学共享资源(P&MSR) 蛋白质组学和代谢组学共享资源(P&MSR)由以FH(FH)为基础的 蛋白质组学基金和华盛顿大学南湖联盟(UW-SLU)代谢组学基金。这个 P&MSR蛋白质组学设施成立于2002年，自成立以来一直在Phil Gafken博士的指导下 盗梦空间。P&MSR代谢研究所成立于2013年，一直由Dr。 丹尼尔·拉夫里。P&MSR的使命是(1)提供高质量、经济高效和可靠的服务 及时进行蛋白质组学和代谢组学实验；(2)开发和实施新的组学 用于揭示影响癌症生物学的分子细节的实验、分析和工具；以及(3)支持 可翻译和临床试验的发展。P&MSR操作和维护层析、质量 和核磁共振仪器，并提供实验设计等服务 和咨询、样品制备和层析、蛋白质或代谢物鉴定和 定量、蛋白质修饰表征、目标蛋白质或代谢物定量、代谢通量 分析、数据分析和信息学支持。新技术和新服务不断涌现 根据联盟成员的反馈进行评估。在下一个批准期内，感兴趣的领域 将进行的研究包括分析主要组织相容性复合肽以支持癌症 免疫学和免疫疗法研究。对于代谢组学，我们将通过以下方式将其添加到我们目前的20多种分析中 扩展我们测量代谢流量的能力，提供研究实时新陈代谢的能力 使用稳定的同位素示踪剂进行研究，并开发更高吞吐量的全球概况分析以支持研究 在癌症生物学方面。凭借完善的运营策略，财团用户拥有经济高效、可靠和 立即进入P&MSR。由P&MSR支持的研究表明，它是一个重要的 支持弗雷德·哈奇/华盛顿大学所需的关键共享资源基础设施的一部分 癌症联盟。

PROJECT SUMMARY: PROTEOMICS & METABOLOMICS SHARED RESOURCE (P&MSR) The Proteomics and Metabolomics Shared Resource (P&MSR) consists of the Fred Hutch (FH)-based Proteomics Facility and the University of Washington South Lake Union (UW-SLU) Metabolomics Facility. The P&MSR Proteomics Facility was formed in 2002 and has been under the direction of Dr. Phil Gafken since its inception. The P&MSR Metabolomics Facility was formed in 2013 and has been under the direction of Dr. Daniel Raftery. The mission of the P&MSR is (1) to provide high-quality, cost-effective, and reliable service in a timely manner for proteomics and metabolomics experiments; (2) to develop and implement new omics experiments, assays, and tools to uncover the molecular details influencing cancer biology; and (3) to support the development of translatable and clinical tests. The P&MSR operates and maintains chromatography, mass spectrometry, and nuclear magnetic resonance instruments and offers services such as experimental design and consultation, sample preparation and chromatography, protein or metabolite identification and quantification, protein modification characterization, targeted protein or metabolite quantification, metabolic flux analysis, and data analysis and informatics support. New technologies and services are continually being evaluated based on feedback from Consortium members. Over the next granting period, areas of interest that will be investigated include the analysis of major histocompatibility complex peptides to support cancer immunology and immunotherapy research. For metabolomics we will add to our over 20 current assays by expanding our capabilities to measure metabolic flux, provide the capability to investigate real-time metabolism studies using stable isotope tracers, and develop higher throughput global profiling assays to support research in cancer biology. With well-established operation policies, Consortium users have cost-effective, reliable, and immediate access to the P&MSR. The research supported by the P&MSR demonstrates that it is an important part of the critical Shared Resources infrastructure needed to support the Fred Hutch/University of Washington Cancer Consortium.