Base-resolution sequencing of 6mA in eukaryotic DNA

真核 DNA 中 6mA 的碱基分辨率测序

基本信息

  • 批准号:
    9769836
  • 负责人:
  • 金额:
    $ 22.13万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-09-01 至 2021-02-28
  • 项目状态:
    已结题

项目摘要

N6-methyladenine (6mA) was recently identified as a new base modification in metazoan genomic DNA. Methylation on adenine was previously thought to be restricted to unicellular organisms. The identification of this mark in metazoans has raised questions about the role of 6mA in metazoan biology. Some studies suggest that 6mA may correlate with developmental regulation, active transcription start sites, or transposon expression, and potentially carry non-genetic information across generations. However, 6mA is a rare DNA modification in eukaryotes and its role in regulating biological processes is still unclear. Because of the low level of 6mA in eukaryotes, some researchers have questioned whether it plays a role in regulating biological processes or is an evolutionary holdover from bacteria. Studies of 6mA have been slowed by a lack of practical methods for accurately examining 6mA locations. Current epigenomic mapping techniques are either prohibitively expensive or do not provide sufficient resolution and accuracy to map the rare 6mA occurrences in metazoan genomes. Here we propose to develop a new technique for identifying sites of adenine methylation genome-wide at nucleotide resolution in a cost-effect manner. We will determine the utility of this technique first in bacteria and then apply it to examine 6mA distribution during development in zebrafish, where we have found that it changes dramatically. This technique will provide a new tool for scientists to define 6mA sites in metazoans to enable the study of its potential role in epigenetics, imprinting and development.
n6 -甲基腺嘌呤(n6 - methylladenine, 6mA)是近年来在后生动物基因组中发现的一种新的碱基修饰

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Eric Lieberman Greer其他文献

Biological roles of adenine methylation in RNA
腺嘌呤甲基化在 RNA 中的生物学作用
  • DOI:
    10.1038/s41576-022-00534-0
  • 发表时间:
    2022-10-19
  • 期刊:
  • 影响因子:
    52.000
  • 作者:
    Konstantinos Boulias;Eric Lieberman Greer
  • 通讯作者:
    Eric Lieberman Greer

Eric Lieberman Greer的其他文献

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{{ truncateString('Eric Lieberman Greer', 18)}}的其他基金

Ribosomal RNA Methylation Regulation of Longevity and Stress Resistance
核糖体 RNA 甲基化对长寿和抗应激的调节
  • 批准号:
    10781428
  • 财政年份:
    2023
  • 资助金额:
    $ 22.13万
  • 项目类别:
Ribosomal RNA methylation regulation of longevity and stress resistance
核糖体RNA甲基化对长寿和抗压能力的调节
  • 批准号:
    10688324
  • 财政年份:
    2022
  • 资助金额:
    $ 22.13万
  • 项目类别:
Ribosomal RNA methylation regulation of longevity and stress resistance
核糖体RNA甲基化对长寿和抗压能力的调节
  • 批准号:
    10793898
  • 财政年份:
    2022
  • 资助金额:
    $ 22.13万
  • 项目类别:
Cap specific N6 methylation of viral mRNA by the cellular methyltransferase PCIF1
细胞甲基转移酶 PCIF1 对病毒 mRNA 进行帽特异性 N6 甲基化
  • 批准号:
    10647750
  • 财政年份:
    2021
  • 资助金额:
    $ 22.13万
  • 项目类别:
Cap specific N6 methylation of viral mRNA by the cellular methyltransferase PCIF1
细胞甲基转移酶 PCIF1 对病毒 mRNA 进行帽特异性 N6 甲基化
  • 批准号:
    10456693
  • 财政年份:
    2021
  • 资助金额:
    $ 22.13万
  • 项目类别:
Characterization of DNA N6-methyl adenine and its role in epigenetic memory
DNA N6-甲基腺嘌呤的表征及其在表观遗传记忆中的作用
  • 批准号:
    10001093
  • 财政年份:
    2016
  • 资助金额:
    $ 22.13万
  • 项目类别:
Characterization of DNA N6-methyl adenine and its role in epigenetic memory
DNA N6-甲基腺嘌呤的表征及其在表观遗传记忆中的作用
  • 批准号:
    9976664
  • 财政年份:
    2016
  • 资助金额:
    $ 22.13万
  • 项目类别:
Characterization of DNA N6-methyl adenine and its role in epigenetic memory
DNA N6-甲基腺嘌呤的表征及其在表观遗传记忆中的作用
  • 批准号:
    9165246
  • 财政年份:
    2016
  • 资助金额:
    $ 22.13万
  • 项目类别:
Identifying the molecular mechanisms of transgenerational epigenetic inheritance
鉴定跨代表观遗传的分子机制
  • 批准号:
    9110197
  • 财政年份:
    2015
  • 资助金额:
    $ 22.13万
  • 项目类别:
Identifying the molecular mechanisms of transgenerational epigenetic inheritance
鉴定跨代表观遗传的分子机制
  • 批准号:
    9069262
  • 财政年份:
    2015
  • 资助金额:
    $ 22.13万
  • 项目类别:

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