Comprehensive Glycoproteomic Tool Development for Cancer Biomarkers

项目摘要 糖基化是蛋白质最普遍和最重要的翻译后修饰之一，但它 也是最难研究的问题之一。任何单一的方法或分析平台都不能给出完整的 有关蛋白质样品中糖基化的信息和目前使用的分析方法包括这两种 ESI和MALDI单质谱和串联质谱仪，至少四种类型的液相色谱，迭代 酶分析，荧光读出的糖链结合实验，以及离子等新方法 流动光谱分析。目前还没有软件平台可以集成这两种不同的 数据通道。在这个项目中，我们建议构建一个名为GlyForm的软件产品，它将能够 分析、可视化和集成多个渠道的糖基化数据。糖型将能够 确定释放的葡聚糖达到证据所保证的详细程度，并使用任一质量对其进行定量 光谱或荧光信号。该软件将包括供专家使用的交互式用户界面 检查，以及定制和自动化的糖基化报告，以供共享和存档。首字母 糖形式的释放将处理单质谱和串联质谱图、层析洗脱时间和 葡聚糖结合实验；稍后将根据客户需求添加其他数据通道。

Project Summary Glycosylation is one of the most prevalent and important post-translational modifications of proteins, yet it is also one of the most difficult to study. No single method nor analytical platform can give complete information about the glycosylation in a protein sample, and the analytical methods in current use include both ESI and MALDI single and tandem mass spectrometry, at least four types of liquid chromatography, iterative enzyme analysis, glycan-binding experiments with fluorescence read-out, and new methods such as ion mobility spectrometry. There is currently no software platform that can integrate even two of these different data channels. In this project we propose to build a software product called GlycoForm that will be capable of analyzing, visualizing, and integrating glycosylation data over multiple channels. GlycoForm will be able to identify released glycans to the level of detail warranted by the evidence and quantify them using either mass spectrometry or fluorescence signals. The software will include both an interactive user interface for expert inspection, and also customized and automated glycosylation reports for sharing and archiving. The initial release of GlycoForm will handle single and tandem mass spectrometry, chromatographic elution time, and glycan-binding experiments; other data channels will be added later in response to customer demand.