ShEEP Request for BZX Fluorescence Microscope

ShEEP 请求 BZX 荧光显微镜

• 批准号: 9905078
• 负责人: Shijie Song
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9905078
• 关键词: 15 year old; 3-Dimensional; Affect; Alzheimer' s Disease; Biomedical Research; Clinical; Development; Diabetes Mellitus; Disease; Equipment; Florida; Fluorescence; Funding; Goals; Grant; Gulf War; Healthcare; Image; Individual; Investigation; Journals; Laboratories; Lighting; Malignant Neoplasms; Measurement; Medical center; Microscope; Mission; Noise; Obesity; Optics; Positioning Attribute; Productivity; Publications; Quality of Care; Regenerative Medicine; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Scanning; Scientist; Sheep; Signal Transduction; Slide; Specific qualifier value; Specimen; Structure; System; Thick; Time; Translational Research; Transportation; Traumatic Brain Injury; United States Department of Veterans Affairs; United States National Institutes of Health; Universities; Veterans; Veterans Hospitals; career; college; fluorescence microscope; instrument; instrumentation; novel; operation; preference; programs; respiratory; synergism

The proposal requests KEYENCE BZ-X810 Fluorescence Microscope as a common resource shared major equipment integral to the core lab facility to be used by biomedical researchers of the James A Haley Veterans Hospital (JAHVH). Biomedical research at JAHVH is conducted by renowned, well-funded investigators. The proposed instrument will be predominantly used by 12 investigators, including 2 Senior Research Career Scientists and 2 Research Career Scientists with ongoing research programs related to Alzheimer's Disease, cancer, diabetes, obesity, Gulf War Illness, respiratory syncytial disease, traumatic brain injury and regenerative medicine. In addition, these individuals have other grants (NIH, AHA, FLDOH, DoD, etc.) that will utilize the fluorescence microscope to help build on existing and new research projects that will benefit Veterans. This acquisition will be a much-needed replacement for the greater than 15-year- old defunct microscope presently available for use. No other multi-functional fluorescence microscopes are available within JAHVH and the one available at the University of South Florida (USF, our affiliate university) is in high-demand within USF, shared by 5 colleges and often time-slots are unavailable as VA researchers are not given preference over USF researchers. Additionally, transportation to USF causes delays adversely affecting samples. This fluorescence microscope has a lot of benefits: Compact size saves benchtop space; Easy operation for all users; Sensitive optics deliver high-quality results; Specify measurement range; Automatically batch capture desired images; High-content analysis with uniform conditions; multi-dimensional image capture with-high-resolution-stitching and Z-stacking; Structured illumination eliminates fluorescence blurring and delivers clear images in just one click; Capture an entire specimen automatically by registering the coordinates of its outermost positions; Accurate analysis of structure and 3D signals. The multi-functional fluorescence microscope captures images at a higher resolution with high sensitivity compared to conventional microscope. Multi-function is built all-in-one, the dark room is not needed. It can scan multi-slides at the same time. Other benefits of this microscope include enhanced signal-to-noise ratio, comprehensible and close examination of thick samples, High quality images are the crux of research projects and are central to publications in high impact journals and obtaining federal funding. Moreover, the instrumentation will accelerate and enhance the research productivity of the JAHVH investigators, allow for development of new research directions, increase synergy between investigators, and increase the translational research capacity of the JAHVH Research Program. Overall, the proposed instrument will significantly impact and augment VAMC's mission to advance the healthcare of Veterans by allowing VA researchers to conduct cutting-edge research in basic, translational or clinical fields. These endeavors will substantially contribute to the long-term research goals of the JAHVH.

该提案要求基恩士BZ-X810荧光显微镜作为共同资源 生物医学研究人员使用的核心实验室设施不可或缺的共用主要设备， James A Haley Veterans Hospital（JAHVH）JAHVH的生物医学研究由 知名的资金雄厚的调查员拟议的文书将主要用于12个 研究者，包括2名高级研究职业科学家和2名研究职业科学家 与正在进行的研究计划有关的阿尔茨海默氏症，癌症，糖尿病，肥胖，海湾 战争疾病，呼吸道合胞体疾病，创伤性脑损伤和再生医学。在 此外，这些人还有其他赠款（NIH、AHA、FLDOH、DoD等）它将利用 荧光显微镜，以帮助建立在现有的和新的研究项目，这将有利于 老兵 这次收购将是一个急需的替代大于15岁的倒闭 显微镜目前可供使用。没有其他多功能荧光显微镜 在JAHVH和南佛罗里达大学（USF，我们的 附属大学）在USF内需求量很大，由5所学院共享，并且通常时间段 由于VA研究人员没有优先于USF研究人员，因此无法获得。此外，本发明还 运输到USF会导致延迟，从而对样品产生不利影响。 这款荧光显微镜有很多优点：紧凑的尺寸节省了工作台空间; 为所有用户提供操作;灵敏的光学器件提供高质量的结果;指定测量范围; 自动批量捕获所需图像;在统一条件下进行高内容分析; 具有高分辨率拼接和Z堆叠的多维图像捕获;结构化 照明消除了荧光模糊，只需点击一下即可提供清晰的图像; 通过记录其最外位置的坐标来自动记录整个样品; 精确分析结构和3D信号。 多功能荧光显微镜以更高的分辨率捕获图像， 与传统显微镜相比，多功能一体机、暗室 是不需要的。它可以同时扫描多张幻灯片。该显微镜的其他优点 包括提高信噪比，对厚样品进行可理解和仔细检查， 高质量的图像是研究项目的关键，也是高质量出版物的核心。 影响期刊和获得联邦资助。此外，仪器将加速， 提高JAHVH研究人员的研究生产力，允许开发新的 研究方向，增加研究人员之间的协同作用，并增加翻译 JAHVH研究计划的研究能力。 总的来说，拟议的工具将大大影响和增强VAMC的使命， 通过允许VA研究人员进行尖端的 基础、转化或临床领域的研究。这些努力将大大有助于 JAHVH的长期研究目标。