ShEEP Request for Zeiss Axio Scan Z1 Digital Scanner

摘要 生物医学研究中最常用的技术之一是检查 干预前后实验动物的组织。扫描，数字化 而分析这些组织病理切片是一个不可或缺的部分，并被广泛使用 在我们的退伍军人管理局生物医学研究项目中。这通常涉及到各种组织。 显微镜下观察切片，并进行图像分析。目前， 这是手动执行的，并以不同的分辨率执行，分别扫描和 量化的。这是一个非常耗时且效率低下的过程。显著地 改进我们的成像和分析过程，我们需要一张高分辨率的幻灯片 扫描仪和分析器，它将立即作为一组 十名退伍军人事务部调查人员。因此，这种系统的可用性是非常理想的，并且 最终将使大量退伍军人管理局调查人员受益。拟议中的新Axio 系统将允许以高分辨率进行自动扫描、归档和分析 这些幻灯片。这种自动化设备可以快速获取高倍率图像，并 将它们连接成大型复合材料，并可以生成整个器官的3D重建 取自连续的组织切片。除了这些独特的功能外，这个系统更容易 与传统显微镜相比，由于缺乏活动部件和直观的控制 由于其封闭式设计和自校准光学元件，因此更易于维护。 这些功能极大地降低了操作员和维护成本。这个装置不仅会 减少组织处理的工作时间和成本，但也将产生更高的质量 用于演示和分析的图像，推动了以退伍军人为中心的广泛 圣地亚哥退伍军人医院的研究更接近临床，直接造福于退伍军人 健康。我们的VASDHS显微成像核心设施没有自动化的， 用于扫描、存档和分析这些组织病理切片的网络系统。 由于缺乏适当的设备，导致大量的体力劳动，而且常常是滑梯 必须以不同的分辨率重新扫描。总而言之，这个系统将是有益的 给放射学、麻醉学、药理学、神经学、外科的研究人员， 心脏病和泌尿学，也将是其他研究人员的有用资源。 。

Abstract One of the most commonly used techniques in biomedical research is examination of tissues from experimental animals before and after interventions. Scanning, digitizing and analyzing these histopathological slides is an integral part and extensively used technology in our VA biomedical research projects. This usually involves various tissue sections examined under the microscope and subsequent image analysis. Currently, this is performed manually and at different resolutions each separately scanned and quantitated. This is a very time consuming and inefficient process. To significantly improve our imaging and analysis process, we are requesting a high- resolution slide scanner and analyzer that will serve immediately as a shared equipment for a group of ten VA investigators. Availability of such a system is therefore highly desirable and would benefit eventually a large number of VA investigators. The proposed new Axio system will permit automated scanning, at high resolution, archiving and analyzing these slides. This automated device rapidly acquires high-magnification images and joins them into large composites and can generate 3D reconstructions of whole organs from serial tissue sections. In addition to these unique capabilities, this system is easier to use than a traditional microscope due to a lack of moving parts and intuitive control software and is easier to maintain due its enclosed design and self-calibrating optics. These features greatly reduce operator and maintenance costs. This device will not only reduce work hours and costs for tissue processing but will also generate higher quality images for presentation and analysis, advancing a wide range of veteran-centric research at the San Diego VA closer toward the clinic and a direct benefit to veterans' health. Our VASDHS micro-imaging core facility does not have an automated, networked system for scanning, archiving and analyzing these histopathological slides. This lack of proper equipment results in extensive manual labor and often the slides have to be re-scanned at different resolutions. In summary, this system will be beneficial to investigators in Radiology, Anesthesiology, Pharmacology, Neurology, Surgery, Cardiology and Urology and will be a useful resource to other investigators as well. .