ShEEP Request for Zeiss Axio Scan Z1 Digital Scanner

ShEEP 请求蔡司 Axio Scan Z1 数字扫描仪

• 批准号: 9905892
• 负责人: MAHADEVAN Raj RAJASEKARAN
• 金额: --
• 依托单位: VA SAN DIEGO HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9905892
• 关键词: 3-Dimensional; Anesthesiology; Animals; Archives; Automation; Award; Biomedical Research; Cardiology; Clinic; Color; Computer software; Consumption; Core Facility; Coupled; Detection; Devices; Diagnostic Procedure; Environment; Equipment; Freedom; Funding; Health; Histopathology; Hour; Image; Image Analysis; In Situ Hybridization; Individual; Intervention; Intuition; Laboratories; Light; Maintenance; Manuals; Microscope; Microscopy; Molecular; Neurology; Operative Surgical Procedures; Optics; Organ; Pharmacology; Process; Productivity; Program Evaluation; Proteomics; Publishing; Radiology Specialty; Research; Research Personnel; Research Project Grants; Resolution; Resources; Running; Sampling; Scanning; Services; Slide; Source; Specimen; Speed; Spinal Cord; Stains; Syncope; System; Techniques; Technology; Time; Tissues; Transportation; Urology; Veterans; Work; base; cost; design; digital; fluorescence imaging; imaging system; improved; programs; reconstruction; spectrograph; tissue processing

Abstract One of the most commonly used techniques in biomedical research is examination of tissues from experimental animals before and after interventions. Scanning, digitizing and analyzing these histopathological slides is an integral part and extensively used technology in our VA biomedical research projects. This usually involves various tissue sections examined under the microscope and subsequent image analysis. Currently, this is performed manually and at different resolutions each separately scanned and quantitated. This is a very time consuming and inefficient process. To significantly improve our imaging and analysis process, we are requesting a high- resolution slide scanner and analyzer that will serve immediately as a shared equipment for a group of ten VA investigators. Availability of such a system is therefore highly desirable and would benefit eventually a large number of VA investigators. The proposed new Axio system will permit automated scanning, at high resolution, archiving and analyzing these slides. This automated device rapidly acquires high-magnification images and joins them into large composites and can generate 3D reconstructions of whole organs from serial tissue sections. In addition to these unique capabilities, this system is easier to use than a traditional microscope due to a lack of moving parts and intuitive control software and is easier to maintain due its enclosed design and self-calibrating optics. These features greatly reduce operator and maintenance costs. This device will not only reduce work hours and costs for tissue processing but will also generate higher quality images for presentation and analysis, advancing a wide range of veteran-centric research at the San Diego VA closer toward the clinic and a direct benefit to veterans' health. Our VASDHS micro-imaging core facility does not have an automated, networked system for scanning, archiving and analyzing these histopathological slides. This lack of proper equipment results in extensive manual labor and often the slides have to be re-scanned at different resolutions. In summary, this system will be beneficial to investigators in Radiology, Anesthesiology, Pharmacology, Neurology, Surgery, Cardiology and Urology and will be a useful resource to other investigators as well. .

摘要 生物医学研究中最常用的技术之一是检查 实验动物在干预前后的组织。扫描、数字化 分析这些组织病理学切片是一个不可或缺的部分， 技术在我们的VA生物医学研究项目。这通常涉及各种组织 在显微镜下检查切片并随后进行图像分析。目前， 这是手动执行的，并且以不同的分辨率分别进行扫描， 定量。这是一个非常耗时和低效的过程。显著 为了改进我们成像和分析过程，我们需要一张高分辨率的幻灯片 扫描仪和分析仪，将立即作为一组共享设备， 十名退伍军人事务部调查员因此，这种系统的可用性是高度期望的， 最终会让很多退伍军人事务部的调查员受益新的Axio 系统将允许高分辨率自动扫描、归档和分析 这些幻灯片。这种自动化设备可以快速获取高放大率图像， 将它们连接成大型复合材料，并可以生成整个器官的3D重建 从连续的组织切片中除了这些独特的功能，该系统更容易 由于缺少活动部件和直观的控制， 由于其封闭式设计和自校准光学元件，因此更易于维护。 这些功能大大降低了操作员和维护成本。该装置不仅 减少组织处理的工作时间和成本，但也将产生更高的质量 用于演示和分析的图像，推进以退伍军人为中心的广泛的 圣地亚哥退伍军人管理局的研究更接近诊所，并直接使退伍军人受益 健康我们的VASDHS显微成像核心设施没有自动化， 用于扫描、存档和分析这些组织病理学切片的网络系统。 缺乏适当的设备导致大量的体力劳动， 必须以不同的分辨率重新扫描。总之，这一制度将是有益的， 放射学、麻醉学、药理学、神经学、外科学、 心脏病学和泌尿外科，并将是一个有用的资源，其他研究人员以及。 .