The State of Connecticut Department of Public Health Retail Food Program

康涅狄格州公共卫生部 PAR-16-099 NARMS加强和加强零售食品样本抗生素耐药性监测的合作协议计划 项目摘要/摘要 康涅狄格州公共卫生部(CT DPH)州立公共卫生实验室(SPHL) 参与国家抗菌素耐药性监测系统(NARMS)零售肉类项目 从2002年到2016年通过食品和药物管理局/兽医中心(FDA/CVM)。 本次监测的目的是监测年内抗菌素耐药性的流行情况和趋势。 食源性沙门氏菌、弯曲杆菌、大肠杆菌和肠球菌。此应用程序将 寻求资金支持CT DPH SPHL参与NARMS抗生素耐药性监测 零售食品样品包括一名指定的人员，他们将负责这方面的所有工作 监视系统。这一职位的具体职责将包括：(A)从10个国家采购40个零售肉类样本 在康涅狄格州各地随机选择的杂货店每月两次，总共80个样本， 样本包括40个鸡肉部分，20个火鸡末，10个牛肉末和10个猪排(B) 鉴定沙门氏菌的检测阶段，包括初始增菌和培养检测， 按照FDA/CVM(C)提供的规程分离弯曲杆菌、大肠埃希菌和肠球菌 将所有样品信息和结果输入实验室信息管理系统(LIMS) 并完成所有NARMS所需的文书工作；(D)每月将所有分离物运往FDA/CVM，以便 血清分型、抗菌药物敏感性测试和基因分析(E)提交所有要求的非抗炎药 电子文书工作(F)履行必要的质量保证/质量控制职能。 此外，CT DPH SPHL将对所有沙门氏菌进行全基因组测序， 弯曲杆菌，大肠埃希氏菌和肠球菌分离。所有的全基因组测序都将 在高级分子检测(AMD)部分执行。2个或更多WG匹配的集群 临床分离株或临床分离株和食品分离株(涉及CT的本地或多个州)会在当天通知 国家实验室向CT流行病学家提供帮助，以便进行快速和完整的调查。2014年9月1日起- 2016年8月31日，CT DPH SPHL为该项目处理了1760个样本，包括：820只鸡，440个地面 火鸡，250块碎牛肉和250块猪排。CT DPH SPHL回收77株沙门氏菌和 从康涅狄格州杂货店购买的零售肉类样品中分离到61株弯曲杆菌 2014年9月1日至2016年8月31日。CT DPH SPHL期待着参与这一计划，以提供数据 用于改进食源性细菌耐药性的检测和监测。

The State of Connecticut Department of Public Health PAR-16-099 NARMS Cooperative Agreement Program to Enhance and Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens Project Summary/Abstract The Connecticut Department of Public Health (CT DPH) State Public Health Laboratory (SPHL) participated in the National Antimicrobial Resistance Monitoring System (NARMS) retail meat program through Food and Drug Administration/Center for Veterinary Medicine (FDA/CVM) from 2002 to 2016. The purpose of this surveillance was to monitor the prevalence and trends of antimicrobial resistance in foodborne isolates of Salmonella, Campylobacter, Escherichia coli and Enterococcus. This application will seek funding to support CT DPH SPHL’s participation in the NARMS antibiotic resistance surveillance of retail food samples including a designated personnel who will be responsible for all aspects of this surveillance. Specific duties of this position will include: (a) purchasing 40 retail meat samples from ten randomly selected grocery stores throughout Connecticut twice a month for a total of 80 samples, samples include 40 chicken parts, 20 ground turkey, 10 ground beef and 10 pork chops (b) performing all phases of testing including initial enrichments and culture testing for the identification of Salmonella, Campylobacter, Escherichia coli and Enterococcus isolates following protocols provided by FDA/CVM (c) entering all sample information and results into the Laboratory Information Management System (LIMS) and completing all NARMS required paperwork; (d) shipping all isolates monthly to FDA/CVM for serotyping, antimicrobial susceptibility testing, and genetic analysis (e) submitting all required NARMS paperwork electronically (f) performing necessary quality assurance/quality control functions. Additionally, the CT DPH SPHL will perform whole genome sequencing (WGS) on all of Salmonella, Campylobacter, Escherichia coli and Enterococcus isolates. All whole genome sequencing will be performed in the Advanced Molecular Detection (AMD) section. Clusters of 2 or more WGS-matched isolates, clinical or clinical and food, (local or multistate involving CT) prompt same-day notification from State Laboratory to CT epidemiologists to facilitate a rapid and complete investigation. From 9/1/2014 – 8/31/2016, the CT DPH SPHL processed 1760 samples for this project including: 820 chicken, 440 ground turkey, 250 ground beef, and 250 pork chops. The CT DPH SPHL recovered 77 isolates of Salmonella and 61 isolates of Campylobacter from retail meat samples purchased at Connecticut grocery stores from 9/1/2014 to 8/31/2016. The CT DPH SPHL looks forward to participation in this program to provide data for the improvement of the detection and surveillance of resistance among foodborne bacteria.