The State of Connecticut Department of Public Health Retail Food Program

康涅狄格州公共卫生部 PAR-16-099 NARMS合作协议计划，以提高和加强零售食品样本中的抗生素耐药性监测 项目概要/摘要 康涅狄格州公共卫生部（CT DPH）国家公共卫生实验室（SPHL） 参与国家抗菌素耐药性监测系统（NARMS）零售肉类计划 2002年至2016年通过食品药品监督管理局/兽药中心（FDA/CVM）。 该监测的目的是监测以下人群的抗菌药物耐药性的流行率和趋势： 沙门氏菌、弯曲杆菌、大肠杆菌和肠球菌的食源性分离株。此应用程序将 寻求资金支持CT DPH SPHL参与NARMS抗生素耐药性监测， 零售食品样品，包括指定的人员谁将负责这方面的所有方面， 监视该职位的具体职责将包括：（a）从10个零售肉类样本中购买40个 每月两次随机选择康涅狄格州的杂货店，共80个样本， 样品包括40块鸡肉、20块火鸡肉、10块碎牛肉和10块猪排（B）， 检测阶段，包括初步富集和沙门氏菌鉴定的培养检测， 弯曲杆菌、大肠埃希菌和肠球菌分离株遵循FDA/CVM提供的方案（c） 将所有样本信息和结果输入实验室信息管理系统（LIMS） 并完成所有NARMS要求的文书工作;（d）每月将所有分离株运送至FDA/CVM， 血清分型、抗菌药物敏感性测试和遗传分析（e）提交所有必要的国家危险评估和监测系统 （f）履行必要的质量保证/质量控制职能。 此外，CT DPH SPHL将对所有沙门氏菌进行全基因组测序（WGS）， 弯曲杆菌、大肠杆菌和肠球菌分离株。所有的全基因组测序将是 在高级分子检测（AMD）部分进行。2个或更多WGS匹配的群集 临床分离株或临床和食品分离株（当地或涉及CT的多个州） 国家实验室的CT流行病学家，以促进快速和全面的调查。从2014年9月1日- 2016年8月31日，CT DPH SPHL为该项目处理了1760个样本，包括：820只鸡，440只鸡 火鸡，250块碎牛肉，250块猪排CT DPH SPHL回收了77株沙门氏菌， 从康涅狄格州杂货店购买的零售肉类样品中分离出61株弯曲杆菌， 9/1/2014至8/31/2016。CT DPH SPHL期待参与此计划以提供数据 用于改进食源性细菌耐药性的检测和监测。

The State of Connecticut Department of Public Health PAR-16-099 NARMS Cooperative Agreement Program to Enhance and Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens Project Summary/Abstract The Connecticut Department of Public Health (CT DPH) State Public Health Laboratory (SPHL) participated in the National Antimicrobial Resistance Monitoring System (NARMS) retail meat program through Food and Drug Administration/Center for Veterinary Medicine (FDA/CVM) from 2002 to 2016. The purpose of this surveillance was to monitor the prevalence and trends of antimicrobial resistance in foodborne isolates of Salmonella, Campylobacter, Escherichia coli and Enterococcus. This application will seek funding to support CT DPH SPHL’s participation in the NARMS antibiotic resistance surveillance of retail food samples including a designated personnel who will be responsible for all aspects of this surveillance. Specific duties of this position will include: (a) purchasing 40 retail meat samples from ten randomly selected grocery stores throughout Connecticut twice a month for a total of 80 samples, samples include 40 chicken parts, 20 ground turkey, 10 ground beef and 10 pork chops (b) performing all phases of testing including initial enrichments and culture testing for the identification of Salmonella, Campylobacter, Escherichia coli and Enterococcus isolates following protocols provided by FDA/CVM (c) entering all sample information and results into the Laboratory Information Management System (LIMS) and completing all NARMS required paperwork; (d) shipping all isolates monthly to FDA/CVM for serotyping, antimicrobial susceptibility testing, and genetic analysis (e) submitting all required NARMS paperwork electronically (f) performing necessary quality assurance/quality control functions. Additionally, the CT DPH SPHL will perform whole genome sequencing (WGS) on all of Salmonella, Campylobacter, Escherichia coli and Enterococcus isolates. All whole genome sequencing will be performed in the Advanced Molecular Detection (AMD) section. Clusters of 2 or more WGS-matched isolates, clinical or clinical and food, (local or multistate involving CT) prompt same-day notification from State Laboratory to CT epidemiologists to facilitate a rapid and complete investigation. From 9/1/2014 – 8/31/2016, the CT DPH SPHL processed 1760 samples for this project including: 820 chicken, 440 ground turkey, 250 ground beef, and 250 pork chops. The CT DPH SPHL recovered 77 isolates of Salmonella and 61 isolates of Campylobacter from retail meat samples purchased at Connecticut grocery stores from 9/1/2014 to 8/31/2016. The CT DPH SPHL looks forward to participation in this program to provide data for the improvement of the detection and surveillance of resistance among foodborne bacteria.