The State of Connecticut Department of Public Health Retail Food Program

康涅狄格州公共卫生部 PAR-20-124 NARMS加强零售食品样本抗生素耐药性监测的合作协议计划（U 01） 允许 项目总结/摘要 康涅狄格州公共卫生部（CT DPH）国家公共卫生实验室（SPHL）目前正在 参与国家抗菌素耐药性监测系统（NARMS）零售肉类 通过食品药品监督管理局/兽医中心实施的监测计划 (FDA/CVM）。该监测项目的目标是确定抗生素耐药性的流行情况 沙门氏菌、弯曲杆菌、大肠杆菌、肠球菌、弧菌、气单胞菌和其他乳糖 从美国杂货店购买的零售食品样本中分离出阳性细菌 该监测项目旨在提高对细菌抗生素耐药性的检测， 粮食商品。本申请旨在寻求拨款，以支持该公司参与 NARMS零售肉类监督计划，包括指定人员，负责 监视活动。这一监测项目的活动包括：a）收集和处理20 每个月两次从康涅狄格州预选的杂货店抽取零售肉类样本， 在40个样品中，样品包括10个鸡肉、10个火鸡肉、10个牛肉肉和10个零售猪肉（肉 （B）每月一次从零售肉类的同一商店购买8个海鲜样本， 收集。样品包括2份生虾、3份生鲑鱼和3份生罗非鱼;（c）进行所有阶段的测定， 微生物检测，包括初始富集、二次富集和 零售肉类中沙门氏菌、弯曲菌、大肠杆菌和肠球菌分离鉴定 样品并进行初始富集，然后进行分离弧菌的培养测试， 肠球菌属、气单胞菌属及其他乳糖阳性菌。所有测试均 按照FDA/CVM提供的方案进行;（d）输入所有样本信息和结果 输入国家档案管理系统电子数据表和实验室信息管理系统; 每月将所有分离株运送至FDA/CVM;（f）将所有要求的NARMS电子数据表提交至 （g）履行必要的质量保证/质量控制职能;（h）提交 沙门氏菌、弯曲杆菌和原代大肠杆菌分离株至CT DPH SPHL Advanced 全基因组测序（WGS）的分子检测（AMD）部分;（i）AMD部分提交 每个月都上传到脉冲网络2个或多个WGS密切相关分离株的集群， 临床或临床和食品，（当地或涉及CT的多个州）从州政府当天立即通知 实验室到CT流行病学家，以促进快速和全面的调查。CT DPH SPHL外观 期待参与该计划，为改进检测提供数据， 食源性细菌的耐药性监测。

The State of Connecticut Department of Public Health PAR-20-124 NARMS Cooperative Agreement Program to Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens(U01) Clinical Trials Not Allowed Project Summary/Abstract The Connecticut Department of Public Health (CT DPH) State Public Health Laboratory (SPHL) is currently participating in the National Antimicrobial Resistance Monitoring System (NARMS) Retail Meat Surveillance program through Food and Drug Administration/Center for Veterinary Medicine (FDA/CVM). The goal of this surveillance project is to determine the prevalence of antibiotic resistance among Salmonella, Campylobacter, Escherichia coli, Enterococcus, Vibrio, Aeromonas, and other lactose positive bacteria isolated from retail food samples purchased from grocery stores in the United States This surveillance project is intended to improve the detection of antibiotic resistance among bacteria in food commodities. This application will seek funding to support CT DPH SPHL’s participation in the NARMS retail meat surveillance program including designated personnel who will be responsible for surveillance activities. The activities in this surveillance project include a) collecting and processing 20 retail meat samples from pre-selected grocery stores throughout Connecticut twice a month for a total of 40 samples, samples include 10 chicken, 10 ground turkey, 10 ground beef, and 10 retail pork (ground or chops); (b) purchasing 8 seafood samples once a month from the same stores the retail meat is collected. Samples include 2 raw shrimp, 3 raw salmon and 3 raw tilapias; (c) performing all phases of microbiological testing including initial enrichments, secondary enrichments and culture testing for the identification of Salmonella, Campylobacter, Escherichia coli and Enterococcus isolates from retail meat samples and performing initial enrichments followed by culture testing for the isolation of Vibrio, Enterococcus, Aeromonas and other lactose positive bacteria from seafood samples. All testing is performed following protocols provided by FDA/CVM; (d) entering all sample information and results into the electronic NARMS data sheets and the Laboratory Information Management System (LIMS); (e) shipping all isolates monthly to FDA/CVM; (f) submitting all required NARMS electronic data sheets to FDA monthly; (g) performing necessary quality assurance/quality control functions; (h) submitting Salmonella, Campylobacter, and primary Escherichia coli isolates to the CT DPH SPHL Advanced Molecular Detection (AMD) section for whole genome sequencing (WGS); (i) AMD section submits sequences to NCBI and uploads to PulseNet monthly. Clusters of 2 or more WGS-closely related isolates, clinical or clinical and food, (local or multistate involving CT) prompt same-day notification from State Laboratory to CT epidemiologists to facilitate a rapid and complete investigation. The CT DPH SPHL looks forward to participation in this program to provide data for the improvement of the detection and surveillance of resistance among foodborne bacteria.