Phenotype-driven approach to understanding the function of craniofacial regulators using IMPC-generated mouse strains

使用 IMPC 产生的小鼠品系的表型驱动方法来了解颅面调节器的功能

• 批准号: 9899973
• 负责人: Jeffrey Ohmann Bush
• 金额: $ 74.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9899973
• 关键词: 3-Dimensional; Ablation; Adult; Affect; Algorithms; Alleles; Animal Model; Applications Grants; Area; Binding; Biological Process; Biomedical Research; Birth; Branchial arch structure; Candidate Disease Gene; Catalogs; Cell Adhesion; Cell physiology; Cells; ChIP-seq; Classification; Cleft Palate; Code; Congenital Abnormality; Craniofacial Abnormalities; Data; Data Set; Databases; Defect; Development; E1A-associated p300 protein; Ectoderm; Effectiveness; Embryo; Embryonic Development; Enhancers; Exhibits; Experimental Models; Face; FaceBase; Fetus; Genes; Genetic; Genome; Goals; Health; Homeostasis; House mice; Human; Informatics; International; Knock-out; Knockout Mice; Knowledge; Laboratory mice; Life; Light; Manuals; Maxilla; Mesenchyme; Modeling; Molecular; Morphogenesis; Morphology; Mouse Strains; Mus; Mutant Strains Mice; Mutate; Mutation; Neoplastic Cell Transformation; Organism; Pattern; Perinatal mortality demographics; Phenotype; Physiologic Ossification; Pregnancy; Premaxillary palate; Process; Proteins; Reporting; Research; Role; Secondary Palate; Shapes; Signaling Molecule; Structural Congenital Anomalies; Syndrome; Time; Tissues; Transgenic Organisms; United States National Institutes of Health; Validation; base; conditional mutant; craniofacial; craniofacial complex; craniofacial development; cranium; design; experience; genome-wide; malformation; mammalian genome; microCT; mouse genome; mutant; phenome; phenotypic data; response; suture fusion; transcription factor; transcriptome; transcriptome sequencing

ABSTRACT Many of the datasets resulting from genome-wide approaches lack functional validation in living organisms. While the laboratory mouse is often used as an experimental model, a large number of mouse genes have unknown functions. The International Mouse Phenotyping Consortium (IMPC) is building the first catalogue of mammalian genome function by generating knockout (KO) mouse strains for every protein-coding gene in the genome. Taking advantage of this opportunity, the two co-PIs have designed this proposal in response to NIH PAR-17-005 for phenotyping IMPC embryonic and perinatal lethal KO mouse lines. Our focus is on mutations that affect the craniofacial complex, based on our expertise in modeling craniofacial malformations in the mouse. While craniofacial defects represent one third of all human birth defects, our knowledge of the underlying cellular and molecular mechanisms remains poor. To select mutant mouse lines for in-depth phenotyping of craniofacial abnormalities, we generated algorithms to intersect the current list of IMPC lethal /subviable KO strains exhibiting craniofacial defects with: 1) all genes present in transcriptomes of mouse embryonic craniofacial domains that were either generated in our labs or available in the FaceBase database; and 2) all significant ChIP-Seq peaks for binding of the enhancer-associated protein p300 in mouse embryonic whole faces present in the FaceBase database. By searching the Mouse Genome Informatics (MGI) database for phenotypic data and by prioritizing genes that have unknown or poorly defined roles in craniofacial develop- ment, we restricted the number of chosen genes to N=30. These comprise regulators of various cellular functions that cause embryonic lethality and craniofacial defects when disrupted in the mouse. Preliminary phenotyping of the KO mouse line for Zfhx4, a gene that exhibited highest maxillary enrichment in our RNA- Seq dataset, revealed that all Zfhx4 KO embryos present cleft palate and maxillary hypoplasia, providing proof of concept for the effectiveness of the proposed strategy. Accordingly, we will characterize 30 mutant lines (15 per lab over 5 years) via the following specific aims: AIM 1: Examination and classification of 30 IMPC mutant mouse lines for craniofacial phenotypes. Employing a two-stage phenotyping pipeline, we will categorize the craniofacial defects for each line and narrow the time of onset. AIM 2. Deep characterization of craniofacial phenotypes. Based on three phenotyping platforms, we will uncover the function(s) of the 30 chosen genes in craniofacial development through in-depth analyses. Platform A will characterize early craniofacial anomalies including defects in branchial arch patterning, as well as primary palate morphogenesis and fusion (E8.5-11.5); Platform B will characterize later abnormalities of secondary palate fusion (E11.5-15.5); and Platform C will dissect perturbations of craniofacial shape/morphology and skull ossification. Shedding light on the cellular and molecular processes controlled by genes that are essential for embryonic development and cause human birth defects when disrupted will be vital to the health of the fetus before and long after birth.

抽象的 由全基因组方法产生的许多数据集缺乏在活生物体中的功能验证。 虽然实验室小鼠经常被用作实验模型，但大量的小鼠基因已经被 未知的功能。国际小鼠表型联盟 (IMPC) 正在构建第一个目录 通过针对哺乳动物基因组中的每个蛋白质编码基因生成敲除（KO）小鼠品系来发挥功能 基因组。两位联合PI利用这个机会设计了这个提案，以回应 NIH PAR-17-005 用于对 IMPC 胚胎和围产期致死 KO 小鼠系进行表型分析。我们的重点是 基于我们在颅面畸形建模方面的专业知识，我们发现了影响颅面复合体的突变 鼠标。虽然颅面缺陷占所有人类出生缺陷的三分之一，但我们对颅面缺陷的了解 潜在的细胞和分子机制仍然很差。选择突变小鼠品系进行深入研究 颅面异常的表型分析，我们生成了交叉当前 IMPC 致死列表的算法 /subviable KO 菌株表现出颅面缺陷：1) 小鼠转录组中存在所有基因 胚胎颅面域，要么在我们的实验室中生成，要么在 FaceBase 数据库中可用； 2) 小鼠胚胎中增强子相关蛋白 p300 结合的所有显着 ChIP-Seq 峰 FaceBase 数据库中存在完整的面孔。通过搜索小鼠基因组信息学（MGI）数据库 对于表型数据并优先考虑在颅面发育中具有未知或不明确作用的基因 另外，我们将所选基因的数量限制为 N=30。这些包括各种细胞的调节剂 当小鼠体内的功能受到破坏时，会导致胚胎致死和颅面缺陷。初步的 对 Zfhx4 的 KO 小鼠系进行表型分析，该基因在我们的 RNA 中表现出最高的上颌骨富集度 Seq数据集显示所有Zfhx4 KO胚胎均存在腭裂和上颌发育不全，提供了证据 拟议战略有效性的概念。因此，我们将表征 30 个突变系（15 每个实验室超过 5 年）通过以下具体目标： AIM 1：30 IMPC 的检查和分类 颅面表型突变小鼠系。采用两阶段表型分析流程，我们将 对每条线的颅面缺陷进行分类并缩小发病时间。目标 2. 深度表征 颅面表型。基于三个表型平台，我们将揭示 30 个表型的功能 通过深入分析选择颅面发育中的基因。平台A将提前表征 颅面异常，包括鳃弓图案缺陷以及初级腭形态发生 和融合（E8.5-11.5）；平台 B 将表征继发性腭融合的后期异常 (E11.5-15.5)； 平台 C 将剖析颅面形状/形态和颅骨骨化的扰动。脱落 阐明对胚胎发育至关重要的基因控制的细胞和分子过程 当受到干扰时，会导致人类出生缺陷，这对于胎儿出生前和出生后很长时间的健康至关重要。