Molecular Biology/Molecular Genetics (Core C)

分子生物学/分子遗传学（核心 C）

• 批准号: 9901512
• 负责人: Wendy K Chung
• 金额: $ 22.94万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9901512
• 关键词: Adipocytes; Animal Model; Applications Grants; Area; Back; Basic Science; Bioinformatics; Biological; Biological Assay; Biopsy; Brain; CRISPR/Cas technology; Cell Differentiation process; Cell Line; Cell Lineage; Cell model; Cells; Clinical Research; Cloning; Collaborations; Communities; Consultations; Core Facility; DNA; DNA sequencing; Data; Data Analyses; Derivation procedure; Emerging Technologies; Equipment; Faculty; Fostering; Gap Junctions; Gene Expression; Generations; Genetic Techniques; Genomics; Genotype; Goals; Grant; Human Resources; Hypothalamic structure; Laboratories; Leptin; Libraries; Liver; Manuscripts; Mediation; Medical; Modernization; Molecular; Molecular Biology; Molecular Genetics; Mus; Mutation; Neurosciences; New York; Obesity; Pathogenesis; Patients; Peer Review; Performance; Phenotype; Phylogenetic Analysis; Physiological; Play; Preparation; Price; Productivity; Proteomics; Protocols documentation; Publications; Reagent; Recording of previous events; Referral and Consultation; Research; Research Activity; Research Design; Research Personnel; Research Project Grants; Research Support; Resources; Ribosomal RNA; Role; Sample Size; Services; Site; Skin; Structure of beta Cell of islet; Students; Techniques; Technology; Training; Transgenic Mice; Viral; analytical tool; base; brain research; cell type; comorbidity; cost; design; designer receptors exclusively activated by designer drugs; exome sequencing; experimental study; feeding; genetic manipulation; genome editing; induced pluripotent stem cell; instrumentation; interest; investigator training; member; new technology; next generation sequencing; novel; nutrition; optogenetics; programs; research and development; skills; stem cell technology; stem cells; success; tool; transcriptome sequencing

MBMG Core PROJECT SUMMARY/ABSTRACT The Molecular Biology/Molecular Genetics (MBMG) Core has served the NYORC obesity research community for the past 4 cycles of this grant. The MBMG Core provides critical research support for obesity-related clinical and basic research activities in the area of molecular genetics, bioinformatics, and model organism characterization. The services are widely used and have increased productivity of NORC investigators, while curtailing costs and facilitating access to advanced genomics technologies. The primary objective of this Core is to assist investigators to apply the tools and technologies of molecular genetics and genomics to elucidate the molecular-genetic bases for the pathogenesis and medical/physiological co-morbidities of obesity. The Specific Aims of the MBMG Core are: 1) To facilitate the application of molecular genetics to problems of obesity by providing expert consultation on both study design and applicable molecular biological techniques; 2) To provide standard laboratory services to young investigators and those without formal labs for studies related to obesity; 3) To develop and make available new cutting edge research tools and reagents. To these ends, the MBMG Core offers, among others, consultation and services, including gene expression, DNA extraction, genotyping, DNA and RNA sequencing, bacterial phylogenetic characterization by 16s rRNA, mouse transgenics, stem cell derivation and differentiation, and genome editing with CRISPR Cas9, analytic tools related to these techniques. Core services are of 3 basic types: (i) On-site Direct Services. (ii) Consultative-Collaborative with Institutional Cores. (iii) Consultative-Referral. To gain access to cutting-edge technologies, avoid duplication and to maximally leverage our resources, we collaborate extensively with other local Core facilities and laboratories. Core personnel provide assistance ranging from study design to execution of studies and data interpretation. We particularly emphasize service to young investigators and holders of P&F grants. The Core has been active in developing new research tools and reagents, and has played an essential role in establishing iPS cell technology for our community. Our R&D activities are focused on developing new tools and reagents for brain research and genetic manipulations in cells and model animals. The MBMG Core has been a nexus for intellectual exchange and collaboration, and an important training venue for students, fellows and young faculty. During the past cycle, the Core responded to >3,000 service requests from 34 NORC users supported by 49 grants, in addition to 11 non-ORC members. These services helped investigators generate 35 new grants and 147 publications.

MBMG核心 项目总结/摘要 分子生物学/分子遗传学（MBMG）核心服务于NYORC肥胖研究社区 在过去的四个周期中，这一补助金。MBMG核心为肥胖相关的临床研究提供了关键的研究支持。 分子遗传学、生物信息学和模式生物领域的基础研究活动 特征化这些服务被广泛使用，提高了NORC调查人员的工作效率， 降低成本并促进获得先进的基因组学技术。 本核心的主要目标是帮助研究人员应用分子生物学的工具和技术， 遗传学和基因组学，以阐明发病机制的分子遗传基础， 肥胖的医学/生理共病。MBMG核心的具体目标是：1）促进 分子遗传学在肥胖问题上的应用，为两种研究设计提供专家咨询 及适用的分子生物学技术; 2）为青少年提供标准的实验室服务， 研究人员和那些没有正式实验室的人进行与肥胖有关的研究; 3）开发并提供 新的尖端研究工具和试剂。为此，MBMG核心提供，除其他外， 咨询和服务，包括基因表达、DNA提取、基因分型、DNA和RNA测序， 通过16 s rRNA、小鼠转基因、干细胞衍生和 分化和使用CRISPR Cas9的基因组编辑，与这些技术相关的分析工具。核心 服务有三种基本类型：（一）现场直接服务。(ii)与机构核心进行协商合作。 (iii)咨询转介。为了获得尖端技术，避免重复， 利用我们的资源，我们与其他当地核心设施和实验室进行广泛合作。核心 人员提供从研究设计到研究执行和数据解释的协助。我们 特别强调为年轻的研究人员和P&F赠款持有人提供服务。核心一直积极参与 开发新的研究工具和试剂，并在建立iPS细胞中发挥了重要作用 技术为我们的社区。我们的研发活动集中在开发新的工具和试剂， 细胞和模型动物的研究和遗传操作。MBMG核心一直是 知识交流与合作，是学生、研究员和青年的重要培训场所 教师。在上一个周期，核心服务中心对来自34个NORC用户的3，000多项服务请求作出了回应 除11名非ORC成员外，还有49名赠款。这些服务帮助调查人员产生了35个新的 赠款物147篇。