Molecular Biology/Molecular Genetics (Core C)

分子生物学/分子遗传学（核心 C）

• 批准号: 9901512
• 负责人: Wendy K Chung
• 金额: $ 22.94万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9901512
• 关键词: Adipocytes; Animal Model; Applications Grants; Area; Back; Basic Science; Bioinformatics; Biological; Biological Assay; Biopsy; Brain; CRISPR/Cas technology; Cell Differentiation process; Cell Line; Cell Lineage; Cell model; Cells; Clinical Research; Cloning; Collaborations; Communities; Consultations; Core Facility; DNA; DNA sequencing; Data; Data Analyses; Derivation procedure; Emerging Technologies; Equipment; Faculty; Fostering; Gap Junctions; Gene Expression; Generations; Genetic Techniques; Genomics; Genotype; Goals; Grant; Human Resources; Hypothalamic structure; Laboratories; Leptin; Libraries; Liver; Manuscripts; Mediation; Medical; Modernization; Molecular; Molecular Biology; Molecular Genetics; Mus; Mutation; Neurosciences; New York; Obesity; Pathogenesis; Patients; Peer Review; Performance; Phenotype; Phylogenetic Analysis; Physiological; Play; Preparation; Price; Productivity; Proteomics; Protocols documentation; Publications; Reagent; Recording of previous events; Referral and Consultation; Research; Research Activity; Research Design; Research Personnel; Research Project Grants; Research Support; Resources; Ribosomal RNA; Role; Sample Size; Services; Site; Skin; Structure of beta Cell of islet; Students; Techniques; Technology; Training; Transgenic Mice; Viral; analytical tool; base; brain research; cell type; comorbidity; cost; design; designer receptors exclusively activated by designer drugs; exome sequencing; experimental study; feeding; genetic manipulation; genome editing; induced pluripotent stem cell; instrumentation; interest; investigator training; member; new technology; next generation sequencing; novel; nutrition; optogenetics; programs; research and development; skills; stem cell technology; stem cells; success; tool; transcriptome sequencing

MBMG Core PROJECT SUMMARY/ABSTRACT The Molecular Biology/Molecular Genetics (MBMG) Core has served the NYORC obesity research community for the past 4 cycles of this grant. The MBMG Core provides critical research support for obesity-related clinical and basic research activities in the area of molecular genetics, bioinformatics, and model organism characterization. The services are widely used and have increased productivity of NORC investigators, while curtailing costs and facilitating access to advanced genomics technologies. The primary objective of this Core is to assist investigators to apply the tools and technologies of molecular genetics and genomics to elucidate the molecular-genetic bases for the pathogenesis and medical/physiological co-morbidities of obesity. The Specific Aims of the MBMG Core are: 1) To facilitate the application of molecular genetics to problems of obesity by providing expert consultation on both study design and applicable molecular biological techniques; 2) To provide standard laboratory services to young investigators and those without formal labs for studies related to obesity; 3) To develop and make available new cutting edge research tools and reagents. To these ends, the MBMG Core offers, among others, consultation and services, including gene expression, DNA extraction, genotyping, DNA and RNA sequencing, bacterial phylogenetic characterization by 16s rRNA, mouse transgenics, stem cell derivation and differentiation, and genome editing with CRISPR Cas9, analytic tools related to these techniques. Core services are of 3 basic types: (i) On-site Direct Services. (ii) Consultative-Collaborative with Institutional Cores. (iii) Consultative-Referral. To gain access to cutting-edge technologies, avoid duplication and to maximally leverage our resources, we collaborate extensively with other local Core facilities and laboratories. Core personnel provide assistance ranging from study design to execution of studies and data interpretation. We particularly emphasize service to young investigators and holders of P&F grants. The Core has been active in developing new research tools and reagents, and has played an essential role in establishing iPS cell technology for our community. Our R&D activities are focused on developing new tools and reagents for brain research and genetic manipulations in cells and model animals. The MBMG Core has been a nexus for intellectual exchange and collaboration, and an important training venue for students, fellows and young faculty. During the past cycle, the Core responded to >3,000 service requests from 34 NORC users supported by 49 grants, in addition to 11 non-ORC members. These services helped investigators generate 35 new grants and 147 publications.

MBMG核心 项目摘要/摘要 分子生物学/分子遗传学（MBMG）核心已为Nyorc肥胖研究界服务 在过去的4个循环中。 MBMG核心为与肥胖相关的临床提供关键的研究支持 以及分子遗传学，生物信息学和模型有机体领域的基础研究活动 表征。这些服务被广泛使用，并具有提高NORC调查人员的生产率，而 减少成本并促进获得高级基因组技术的访问。 该核心的主要目的是协助研究人员应用分子的工具和技术 遗传学和基因组学阐明了发病机理和 肥胖的医学/生理合并症。 MBMG核心的具体目的是：1）促进 通过提供两种研究设计的专家咨询，将分子遗传学应用于肥胖问题 和适用的分子生物技术； 2）为年轻人提供标准的实验室服务 研究人员和没有正式实验室的研究人员进行研究； 3）开发和提供 新的尖端研究工具和试剂。对于这些目的，MBMG核心提供了 咨询和服务，包括基因表达，DNA提取，基因分型，DNA和RNA测序， 通过16S rRNA，小鼠转基因，干细胞推导和 通过CRISPR CAS9的分化和基因组编辑与这些技术有关的分析工具。核 服务分为3种基本类型：（i）现场直接服务。 （ii）与机构核心的协商协商。 （iii）咨询 - 转征。要获得最先进的技术，请避免重复并最大程度地重复 利用我们的资源，我们与其他当地核心设施和实验室进行了广泛的合作。核 人员提供从研究设计到执行研究和数据解释的帮助。我们 特别强调为年轻的调查员和P＆F赠款的持有人提供服务。核心已经活跃 开发新的研究工具和试剂，并且在建立IPS细胞中发挥了重要作用 我们社区的技术。我们的研发活动的重点是开发大脑的新工具和试剂 细胞和模拟动物的研究和遗传操作。 MBMG核心一直是 知识交流与协作，以及针对学生，研究员和年轻的重要培训场所 学院。在过去的周期中，核心响应了34位NORC用户支持的3,000个服务请求 除了11个非侵犯成员外，还提供了49个赠款。这些服务帮助调查人员生成了35个新的服务 赠款和147个出版物。